Special Issue "Enzyme Engineering: Current Trends and Future Perspectives"

A special issue of Catalysts (ISSN 2073-4344). This special issue belongs to the section "Biocatalysis".

Deadline for manuscript submissions: 31 December 2021.

Special Issue Editors

Prof. Rénato Froidevaux
E-Mail Website
Guest Editor
University of Lille, Villeneuve-d'Ascq, France
Interests: enzyme catalysis; homogeneous and heterogeneous enzyme catalysis; immobilization; enzyme reactor; hybrid catalysis; biomass valorization; biorefineries
Special Issues and Collections in MDPI journals
Dr. Alexandra Cristina Blaga
E-Mail Website
Guest Editor
Universitatea Tehnica Gheorghe Asachi din Iasi, Romania
Interests: Biocatalysis; Reactive extraction; Pertraction; Microencapsulation

Special Issue Information

Dear Colleagues,

Biocatalysis, due to the recent progress in molecular biotechnology, has reached a particularly exciting point, leading to the appearance of a wide variety of novel enzymes with diverse applications and unique physicochemical properties, based on specialized methods for heterologous enzyme expression and engineering. In order to create new activities or improve existing ones (altered versions of naturally occurring proteins with novel or “tailor-made” properties), new enzyme structures may be designed and produced, based on protein engineering (able to modulate the specificity and stereoselectivity of enzymes).

As green catalysts, enzymes are also fully compatible with environmental and modern health-and-safety standards, being used not only in academia but also in industry (the pharmaceutical and agrochemical industries for optically pure molecules, and the food and chemical industries). Enzyme engineering, based on physical modifications of proteins or modifications at the genetic level, can be used for improving the catalytic efficiencies, specificity and reaction scopes of enzymes for advanced biocatalytic applications. Microbial screening, using high-throughput-screening and the metagenomic analysis of uncultured microorganisms, is a simple and frequently used method for finding new biocatalysts with required properties.

In this Special Issue, we mainly focus on work dealing with the high-throughput screening approach for the discovery and engineering of biocatalysts for optimizing their production in integrated processes and for their extraction/purification

Prof. Rénato Froidevaux
Dr. Alexandra Cristina Blaga
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Catalysts is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2000 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • enzyme applications
  • expression system
  • gene
  • molecular modification
  • biocatalytic applications
  • directed evolution
  • rational design
  • semirational design
  • medium engineering
  • Sources of novel enzymes
  • Bioinformatics approaches for enzyme screening
  • Robotizable technologies
  • High-throughput screening of enzymes
  • Microfluidic devices for enzyme assays
  • Miniscale bioreactor technology
  • High-throughput enzyme purification
  • Molecular and biochemical characterization of enzymes

Published Papers

This special issue is now open for submission.
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