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Biosensors
Special Issues
Biosensing Systems for Allergen and Allergy Testing
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Biosensing Systems for Allergen and Allergy Testing

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensors and Healthcare".

Deadline for manuscript submissions: closed (31 December 2023) | Viewed by 8566

Special Issue Editors

Prof. Dr. Sergi Morais

E-Mail Website
Guest Editor
Departamento de Química, Universitat Politècnica de València, 46022 Valencia, Spain
Interests: allergy; analytical chemistry; biosensors; multiplex immunoassay; PCR
Dr. Amadeo Sena-Torralba

E-Mail
Guest Editor
Departamento de Química, Universitat Politècnica de València, 46022 Valencia, Spain
Interests: point-of-care biosensing; lateral flow immunoassay; nanotechnology; smartphone-based quantification

Special Issue Information

Dear Colleagues,

Over the past years, the numbers of publications related to the detection of food, environmental and drug allergens and allergies in humans and pets have increased rapidly, which implies that analytical tools are playing an important role in this area of knowledge.

With the rapid development of molecular diagnostics, biosensing techniques integrated with new materials and devices have shown great potential for in vitro allergy testing. Biosensing systems and biosensors are analytical devices that use a biological recognition element (e.g., enzyme, antibody, nucleic acids, and aptamer) for the specific detection of target analytes in biological samples and a system to transduce the biorecognition into a measurable signal.

The Special Issue on Biosensing systems for allergen and allergy testing of Biosensors, an Open Access journal from MDPI, will provide insights into novel analytical approaches on relevant principles, materials, and methods to solve shortcomings of current technologies, to bring and stimulate new ideas to one of the most exciting and clinically beneficial areas of research. In short, this Special Issue will provide a platform and opportunity to promote cooperation, information, dissemination and exchange between researchers and industry based on novel biosensing technologies for allergen and allergy testing.

Prof. Dr. Sergi Morais
Dr. Amadeo Sena-Torralba
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • IgE
  • allergen
  • biosensing
  • in vitro testing
  • component-resolved diagnostics

Published Papers (2 papers)

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Research

15 pages, 2868 KiB  
Article
Patterned Biolayers of Protein Antigens for Label-Free Biosensing in Cow Milk Allergy
by Augusto Juste-Dolz, Estrella Fernández, Rosa Puchades, Miquel Avella-Oliver and Ángel Maquieira
Biosensors 2023, 13(2), 214; https://doi.org/10.3390/bios13020214 - 01 Feb 2023
Cited by 2 | Viewed by 1505
Abstract
This paper focuses on creating one-dimensional diffractive grooved structures of antigen proteins on glass substrates for the label-free detection of antibodies to dairy allergens. In particular, the fabrication of protein structures is carried out by combining microcontact printing with physisorption, imines coupling, and [...] Read more.
This paper focuses on creating one-dimensional diffractive grooved structures of antigen proteins on glass substrates for the label-free detection of antibodies to dairy allergens. In particular, the fabrication of protein structures is carried out by combining microcontact printing with physisorption, imines coupling, and thiol-ene click chemistry. The work first sets up these patterning methods and discusses and compares the main aspects involved in them (structure, biolayer thickness, functionality, stability). Homogeneous periodic submicron structures of proteins are created and characterized by diffractive measurements, AFM, FESEM, and fluorescence scanning. Then, this patterning method is applied to proteins involved in cow milk allergy, and the resulting structures are implemented as optical transducers to sense specific immunoglobulins G. In particular, gratings of bovine serum albumin, casein, and β-lactoglobulin are created and assessed, reaching limits of detection in the range of 30–45 ng·mL−1 of unlabeled antibodies by diffractive biosensing. Full article
(This article belongs to the Special Issue Biosensing Systems for Allergen and Allergy Testing)
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14 pages, 2198 KiB  
Article
Lateral Flow Microimmunoassay (LFµIA) for the Reliable Quantification of Allergen Traces in Food Consumables
by Amadeo Sena-Torralba, Javier Gabaldón-Atienza, Aitor Cubells-Gómez, Patricia Casino, Ángel Maquieira and Sergi Morais
Biosensors 2022, 12(11), 980; https://doi.org/10.3390/bios12110980 - 07 Nov 2022
Cited by 4 | Viewed by 6129
Abstract
Quality assurance and food safety are of great concern within the food industry because of unknown quantities of allergens often present in food. Therefore, there is an ongoing need to develop rapid, sensitive, and easy to use methods that serve as an alternative [...] Read more.
Quality assurance and food safety are of great concern within the food industry because of unknown quantities of allergens often present in food. Therefore, there is an ongoing need to develop rapid, sensitive, and easy to use methods that serve as an alternative to mass spectrometry and enzyme-linked immunosorbent assay (ELISA) for monitoring food safety. Lateral flow immunoassay is one of the most used point-of-need devices for clinical, environmental, and food safety applications. Compared to traditional methods, it appears to be a simple and fast alternative for detecting food allergens. However, its reliability is frequently questioned due to the lack of quantitative information. In this study, a lateral flow microimmunoassay (LFµIA) is presented that integrates up to 36 spots in microarray format in a single strip, providing semi-quantitative information about the level of allergens, positive and negative controls, internal calibration, and hook effect. The LFµIA has been evaluated for the on-site simultaneous and reliable quantification of almond and peanut allergens as a proof of concept, demonstrating high sensitivity (185 and 229 µg/kg, respectively), selectivity (77%), and accuracy (RSD 5–25%) when analyzing commercial allergen-suspicious food consumables. Full article
(This article belongs to the Special Issue Biosensing Systems for Allergen and Allergy Testing)
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