Nucleic Acid Amplification-Based Biosensors: Design, Fabrication and Applications

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Nano- and Micro-Technologies in Biosensors".

Deadline for manuscript submissions: closed (20 November 2023) | Viewed by 1762

Special Issue Editor


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Guest Editor
School of Engineering Sciences in Chemistry, Biotechnology, and Health, KTH Royal Institute of Technology, 10044 Stockholm, Sweden
Interests: biosensor; DNA; molecular biology; diagnostics

Special Issue Information

Dear Colleagues,

Nucleic acid amplification methods are the gold standard of molecular diagnostics, as they are more sensitive, specific, and rapid than conventional immunoassays or culturing methods. However, most nucleic acid amplification methods require centralized laboratories and trained personnel and, to a large extent, fail to be a point-of-care. Biosensors based on nucleic acids combine the specificity and sensitivity of the nucleic acid recognition event and amplification, respectively, in a user-friendly, low-cost, compact, and integrated device in order to achieve near-patient or on-site testing. This Special Issue, "Nucleic Acid Amplification-Based Biosensors: Design, Fabrication and Applications", focuses on the recent advances in biosensors that combine a nucleic acid (DNA or RNA) amplification process (thermal or isothermal methods) together with different detection readouts (e.g., colorimetric and electrochemical) and detection strategies (e.g., microarrays, microfluidic paper analytical devices, lateral flow tests, and micro-total analysis systems). We invite submissions of research that help to advance the field of nucleic acid amplification-based biosensors, from the design and fabrication to application in different fields (such as disease diagnosis, health surveillance, food safety, and environmental monitoring).

Dr. Anna Toldrà
Guest Editor

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Keywords

  • biosensor
  • nucleic acid
  • thermal DNA amplification
  • isothermal DNA amplification
  • in situ testing
  • integration
  • detection
  • sample-to-answer

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Published Papers (1 paper)

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Research

10 pages, 1346 KiB  
Article
Toward Continuous Molecular Testing Using Gold-Coated Threads as Multi-Target Electrochemical Biosensors
by Martin Hanze, Shirin Khaliliazar, Pedro Réu, Anna Toldrà and Mahiar M. Hamedi
Biosensors 2023, 13(9), 844; https://doi.org/10.3390/bios13090844 - 25 Aug 2023
Cited by 2 | Viewed by 1367
Abstract
Analytical systems based on isothermal nucleic acid amplification tests (NAATs) paired with electroanalytical detection enable cost-effective, sensitive, and specific digital pathogen detection for various in situ applications such as point-of-care medical diagnostics, food safety monitoring, and environmental surveillance. Self-assembled monolayers (SAMs) on gold [...] Read more.
Analytical systems based on isothermal nucleic acid amplification tests (NAATs) paired with electroanalytical detection enable cost-effective, sensitive, and specific digital pathogen detection for various in situ applications such as point-of-care medical diagnostics, food safety monitoring, and environmental surveillance. Self-assembled monolayers (SAMs) on gold surfaces are reliable platforms for electroanalytical DNA biosensors. However, the lack of automation and scalability often limits traditional chip-based systems. To address these challenges, we propose a continuous thread-based device that enables multiple electrochemical readings on a functionalized working electrode Au thread with a single connection point. We demonstrate the possibility of rolling the thread on a spool, which enables easy manipulation in a roll-to-roll architecture for high-throughput applications. As a proof of concept, we have demonstrated the detection of recombinase polymerase amplification (RPA) isothermally amplified DNA from the two toxic microalgae species Ostreopsis cf. ovata and Ostreopsis cf. siamensis by performing a sandwich hybridization assay (SHA) with electrochemical readout. Full article
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