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Biosensors
Special Issues
Nanoparticle-Based Biosensors for Detection
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Nanoparticle-Based Biosensors for Detection

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensors and Healthcare".

Deadline for manuscript submissions: 30 May 2025 | Viewed by 10262

Special Issue Editor

Dr. Hyeon-Yeol Cho

E-Mail Website
Guest Editor
Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul 02707, Republic of Korea
Interests: stem cell biology; nanobiomaterials
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In recent years, nanoparticle-based biosensors have emerged as a groundbreaking technology with significant potential to detect a wide range of biological molecules, from proteins to DNA sequences. The unique optical, electronic, and magnetic properties of nanoparticles, such as gold nanoparticles, quantum dots, and magnetic nanoparticles, improve sensitivity and specificity in detection assays. These biosensors typically work by combining nanoparticles with biological recognition elements, such as antibodies or nucleic acid sequences. Interaction with the target molecule produces a detectable signal through color change, fluorescence, or magnetic resonance.

Several advantages of nanoparticle-based biosensors include the ability to detect even at very low concentrations, a fast response time, miniaturization, and the potential for integration into portable devices. This has profound implications for point-of-care diagnostics, environmental monitoring, and biomedical research; however, there are challenges that need to be addressed, including safety, standardization, and potential biological effects. Nonetheless, continued advancements in this technology highlight its potential, and a growing number of research articles and reviews are exploring its numerous applications and innovations in greater depth.

This Special Issue aims to publish cutting-edge original articles and comprehensive reviews covering novel nanoparticle-based biosensors. Contributions may include various aspects, such as new designs, manufacturing, chemistry, analysis, application perspectives, or similar topics.

Dr. Hyeon-Yeol Cho
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • functional nanoparticle
  • gold nanoparticle
  • magnetic nanoparticle
  • quantum dots
  • silica nanoparticle
  • biosensor
  • chemical sensors
  • immunosensors
  • electrochemical biosensors

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Published Papers (5 papers)

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Research

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17 pages, 2651 KiB  
Article
Magnetically Localized Detection of Amplified DNA Using Biotinylated and Fluorescent Primers and Magnetic Nanoparticles
by Etienne Orsini, Franz Bruckert, Marianne Weidenhaupt, Orphée Cugat, Paul Kauffmann and Sarah Delshadi
Biosensors 2025, 15(3), 195; https://doi.org/10.3390/bios15030195 - 18 Mar 2025
Viewed by 431
Abstract
Quantitative nucleic acid detection is widely used in molecular diagnostics for infectious diseases. Here, we demonstrate that the previously developed MLFIA (magnetically localized fluorescent immunoassay) has the potential to detect Polymerase Chain Reaction (PCR) and loop-mediated isothermal amplification (LAMP) products using biotinylated and [...] Read more.
Quantitative nucleic acid detection is widely used in molecular diagnostics for infectious diseases. Here, we demonstrate that the previously developed MLFIA (magnetically localized fluorescent immunoassay) has the potential to detect Polymerase Chain Reaction (PCR) and loop-mediated isothermal amplification (LAMP) products using biotinylated and fluorescent primers and streptavidin-coated magnetic nanoparticles. The functionalized nanoparticles separate amplified DNA from non-incorporated primers in situ, allowing the quantification of DNA products. We compare magnetically localized fluorescence detection to commercial technologies based on the DNA intercalation of fluorescent dyes. Our system allows the detection of PCR and LAMP products but is approximately 10 times less sensitive than standard commercial assays. Future optimizations, such as enhancing the signal-to-noise ratio and improving nanoparticle functionalization, could significantly increase sensitivity and bring it closer to current diagnostic standards. This work highlights the potential of magnetically localized fluorescence detection to detect DNA. Full article
(This article belongs to the Special Issue Nanoparticle-Based Biosensors for Detection)
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20 pages, 7845 KiB  
Article
Exploring Distinct Second-Order Data Approaches for Thiamine Quantification via Carbon Dot/Silver Nanoparticle FRET Reversion
by Rafael C. Castro, Ricardo N. M. J. Páscoa, M. Lúcia M. F. S. Saraiva, João L. M. Santos and David S. M. Ribeiro
Biosensors 2024, 14(12), 604; https://doi.org/10.3390/bios14120604 - 10 Dec 2024
Viewed by 832
Abstract
Accurate and selective monitoring of thiamine levels in multivitamin supplements is essential for preventing deficiencies and ensuring product quality. To achieve this, a Förster resonance energy transfer (FRET) system using carbon dots (CDs) as energy donors and citrate-stabilized silver nanoparticles (AgNPs) as energy [...] Read more.
Accurate and selective monitoring of thiamine levels in multivitamin supplements is essential for preventing deficiencies and ensuring product quality. To achieve this, a Förster resonance energy transfer (FRET) system using carbon dots (CDs) as energy donors and citrate-stabilized silver nanoparticles (AgNPs) as energy acceptors was developed. The aqueous synthesis of AgNPs using microwave irradiation was optimized to obtain efficient plasmonic nanoparticles for FRET applications, targeting maximal absorbance intensity, stability, and wavelength alignment. Using a central composite orthogonal design (CCOD), the optimal conditions were identified as a 12.5 min microwave reaction time, a Ag molar ratio of 0.72, and a pH of 8.28. The FRET sensing scheme was applied for thiamine determination, where the vitamin’s presence impaired the FRET process, restoring CDs’ photoluminescence (PL) emission in a concentration-dependent manner. To mitigate interference from other vitamins, PL kinetic data and excitation–emission matrix (EEM) data were analyzed using unfolded partial least-squares (U-PLS) with the subsequent application of the residual bilinearization technique (RBL), achieving high sensitivity and specificity for thiamine detection. This method demonstrated its accuracy and robustness by attaining a determination coefficient (R2) of 0.952 and a relative error of prediction (REP%) of 11%. This novel method offers highly sensitive and interference-free thiamine detection, with significant potential for a wide range of analytical applications. Full article
(This article belongs to the Special Issue Nanoparticle-Based Biosensors for Detection)
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23 pages, 3203 KiB  
Article
Ultrasensitive Lateral Flow Immunoassay of Fluoroquinolone Antibiotic Gatifloxacin Using Au@Ag Nanoparticles as a Signal-Enhancing Label
by Olga D. Hendrickson, Nadezhda A. Byzova, Vasily G. Panferov, Elena A. Zvereva, Shen Xing, Anatoly V. Zherdev, Juewen Liu, Hongtao Lei and Boris B. Dzantiev
Biosensors 2024, 14(12), 598; https://doi.org/10.3390/bios14120598 - 6 Dec 2024
Cited by 1 | Viewed by 1174
Abstract
Gatifloxacin (GAT), an antibiotic belonging to the fluoroquinolone (FQ) class, is a toxicant that may contaminate food products. In this study, a method of ultrasensitive immunochromatographic detection of GAT was developed for the first time. An indirect format of the lateral flow immunoassay [...] Read more.
Gatifloxacin (GAT), an antibiotic belonging to the fluoroquinolone (FQ) class, is a toxicant that may contaminate food products. In this study, a method of ultrasensitive immunochromatographic detection of GAT was developed for the first time. An indirect format of the lateral flow immunoassay (LFIA) was performed. GAT-specific monoclonal antibodies and labeled anti-species antibodies were used in the LFIA. Bimetallic core@shell Au@Ag nanoparticles (Au@Ag NPs) were synthesized as a new label. Peroxidase-mimic properties of Au@Ag NPs allowed for the catalytic enhancement of the signal on test strips, increasing the assay sensitivity. A mechanism of Au@Ag NPs-mediated catalysis was deduced. Signal amplification was achieved through the oxidative etching of Au@Ag NPs by hydrogen peroxide. This resulted in the formation of gold nanoparticles and Ag+ ions, which catalyzed the oxidation of the peroxidase substrate. Such “chemical enhancement” allowed for reaching the instrumental limit of detection (LOD, calculated by Three Sigma approach) and cutoff of 0.8 and 20 pg/mL, respectively. The enhanced assay procedure can be completed in 21 min. The enhanced LFIA was tested for GAT detection in raw meat samples, and the recoveries from meat were 78.1–114.8%. This method can be recommended as a promising instrument for the sensitive detection of various toxicants. Full article
(This article belongs to the Special Issue Nanoparticle-Based Biosensors for Detection)
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11 pages, 8847 KiB  
Article
Solvent-Free and Cost-Efficient Fabrication of a High-Performance Nanocomposite Sensor for Recording of Electrophysiological Signals
by Shuyun Zhuo, Anan Zhang, Alexandre Tessier, Chris Williams and Shideh Kabiri Ameri
Biosensors 2024, 14(4), 188; https://doi.org/10.3390/bios14040188 - 11 Apr 2024
Cited by 5 | Viewed by 4340
Abstract
Carbon nanotube (CNT)-based nanocomposites have found applications in making sensors for various types of physiological sensing. However, the sensors’ fabrication process is usually complex, multistep, and requires longtime mixing and hazardous solvents that can be harmful to the environment. Here, we report a [...] Read more.
Carbon nanotube (CNT)-based nanocomposites have found applications in making sensors for various types of physiological sensing. However, the sensors’ fabrication process is usually complex, multistep, and requires longtime mixing and hazardous solvents that can be harmful to the environment. Here, we report a flexible dry silver (Ag)/CNT/polydimethylsiloxane (PDMS) nanocomposite-based sensor made by a solvent-free, low-temperature, time-effective, and simple approach for electrophysiological recording. By mechanical compression and thermal treatment of Ag/CNT, a connected conductive network of the fillers was formed, after which the PDMS was added as a polymer matrix. The CNTs make a continuous network for electrons transport, endowing the nanocomposite with high electrical conductivity, mechanical strength, and durability. This process is solvent-free and does not require a high temperature or complex mixing procedure. The sensor shows high flexibility and good conductivity. High-quality electroencephalography (EEG) and electrooculography (EOG) were performed using fabricated dry sensors. Our results show that the Ag/CNT/PDMS sensor has comparable skin–sensor interface impedance with commercial Ag/AgCl-coated dry electrodes, better performance for noninvasive electrophysiological signal recording, and a higher signal-to-noise ratio (SNR) even after 8 months of storage. The SNR of electrophysiological signal recording was measured to be 26.83 dB for our developed sensors versus 25.23 dB for commercial Ag/AgCl-coated dry electrodes. Our process of compress-heating the functional fillers provides a universal approach to fabricate various types of nanocomposites with different nanofillers and desired electrical and mechanical properties. Full article
(This article belongs to the Special Issue Nanoparticle-Based Biosensors for Detection)
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Review

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21 pages, 2706 KiB  
Review
Biohybrid Nanoparticle-Based In Situ Monitoring of In Vivo Drug Delivery
by Sohee Ju and Hyeon-Yeol Cho
Biosensors 2023, 13(12), 1017; https://doi.org/10.3390/bios13121017 - 6 Dec 2023
Cited by 4 | Viewed by 2861
Abstract
Nanomaterials have gained huge attention worldwide owing to their unique physicochemical characteristics which enable their applications in the field of biomedicine and drug delivery systems. Although nanodrug delivery systems (NDDSs) have better target specificity and bioavailability than traditional drug delivery systems, their behavior [...] Read more.
Nanomaterials have gained huge attention worldwide owing to their unique physicochemical characteristics which enable their applications in the field of biomedicine and drug delivery systems. Although nanodrug delivery systems (NDDSs) have better target specificity and bioavailability than traditional drug delivery systems, their behavior and clearance mechanisms in living subjects remain unclear. In this regard, the importance of bioimaging methods has come to the forefront for investigating the biodistribution of nanocarriers and discovering drug release mechanisms in vivo. In this review, we introduce several examples of biohybrid nanoparticles and their clinical applications, focusing on their advantages and limitations. The various bioimaging methods for monitoring the fate of nanodrugs in biological systems and the future perspectives of NDDSs have also been discussed. Full article
(This article belongs to the Special Issue Nanoparticle-Based Biosensors for Detection)
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