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Biosensors
Special Issues
Cutting-Edge Nanozyme Biosensing Strategies for Biomedical and Environmental Applications
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Cutting-Edge Nanozyme Biosensing Strategies for Biomedical and Environmental Applications

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor Materials".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 3545

Special Issue Editors

Prof. Dr. Moon Il Kim

E-Mail Website
Guest Editor
Department of BioNano Technology, College of BioNano Technology, Gachon University, Seongnam 13120, Republic of Korea
Interests: nanozyme engineering; biosensors and biochip; nanobiomedical engineering; antimicrobial technology; enzyme engineering
Special Issues, Collections and Topics in MDPI journals
Dr. Phuong Thy Nguyen

E-Mail Website
Guest Editor
Department of BioNano Technology, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam 13120, Gyeonggi, Republic of Korea
Interests: nanozymes; biosensors; paper-based microfluidic device

Special Issue Information

Dear Colleagues,

Nanozymes, defined as nanomaterials with intrinsic enzyme-like activity, have emerged as powerful substitutes for natural enzymes in the design of advanced biosensing platforms. Their remarkable appeal stems from their cost-effectiveness, exceptional stability under diverse conditions, and ability to fine-tune their catalytic performance through straightforward surface modifications. These attributes enable nanozyme engineering with exceptional precision for a wide range of targeted applications, from clinical diagnostics to environmental monitoring. In recent years, nanozyme-based approaches have been successfully implemented for the detection of diverse biomarkers, pathogens, and contaminants, showcasing their versatility, precision, and potential to overcome the limitations of traditional enzymes.

Therefore, this Special Issue, entitled “Cutting-Edge Nanozyme Biosensing Strategies for Biomedical and Environmental Applications”, highlights pioneering research on the detection mechanisms, rational design principles, and practical uses of nanozymes in biomedical and environmental applications. We especially welcome contributions that address the key challenges of nanozyme-based biosensors, such as their sensitivity, selectivity, and reproducibility, as well as studies exploring innovative methods of integration with digital technologies for point-of-care diagnostics. We invite researchers to submit original research articles and insightful reviews to advance fundamental nanozyme research and broaden their applications across various disciplines.

Prof. Dr. Moon Il Kim
Dr. Phuong Thy Nguyen
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • nanozyme
  • catalytic mechanism
  • activity regulation
  • biosensing
  • medical diagnosis
  • point-of-care diagnostics
  • environment monitoring

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Published Papers (4 papers)

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Research

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13 pages, 2327 KB  
Communication
Quantitative IC50 Analysis of Puromycin-Induced Cytotoxicity in NIH/3T3 Cells Using a Multi-Well Array Impedance Biosensor
by Seok-kyu Kim, SuGwon Nam and Moongyu Jang
Biosensors 2025, 15(9), 572; https://doi.org/10.3390/bios15090572 - 1 Sep 2025
Viewed by 795
Abstract
ECIS-based impedance biosensors have been extensively studied in various fields including cancer research, microbiology, and immunology. However, most studies have primarily focused on monitoring cellular behavior through impedance changes, with relatively less emphasis on interpreting the biological significance of impedance signals. In this [...] Read more.
ECIS-based impedance biosensors have been extensively studied in various fields including cancer research, microbiology, and immunology. However, most studies have primarily focused on monitoring cellular behavior through impedance changes, with relatively less emphasis on interpreting the biological significance of impedance signals. In this study, we employed a multi-well array impedance biosensor to conduct IC50 (half-maximal inhibitory concentration) analysis, a widely used metric for evaluating drug efficacy and toxicity in biological and pharmacological research. Specifically, we assessed the IC50 values of puromycin, an aminonucleoside antibiotic known to inhibit protein synthesis. NIH/3T3 fibroblasts were exposed to various concentrations of puromycin, and real-time impedance monitoring was performed. Cell viability was assessed, and the IC50 value of puromycin for NIH/3T3 cells was determined to be 3.96 µM using capacitance-based impedance analysis. Our findings demonstrate that the multi-well array impedance biosensor provides a rapid and quantitative method for drug toxicity evaluation, offering a valuable platform for drug screening and biocompatibility assessment. Full article
(This article belongs to the Special Issue Cutting-Edge Nanozyme Biosensing Strategies for Biomedical and Environmental Applications)
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Review

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29 pages, 3451 KB  
Review
Deep Learning-Enhanced Nanozyme-Based Biosensors for Next-Generation Medical Diagnostics
by Seungah Lee, Nayra A. M. Moussa and Seong Ho Kang
Biosensors 2025, 15(9), 571; https://doi.org/10.3390/bios15090571 - 1 Sep 2025
Viewed by 879
Abstract
The integration of deep learning (DL) and nanozyme-based biosensing has emerged as a transformative strategy for next-generation medical diagnostics. This review explores how DL architectures enhance nanozyme design, functional optimization, and predictive modeling by elucidating catalytic mechanisms such as dual-atom active sites and [...] Read more.
The integration of deep learning (DL) and nanozyme-based biosensing has emerged as a transformative strategy for next-generation medical diagnostics. This review explores how DL architectures enhance nanozyme design, functional optimization, and predictive modeling by elucidating catalytic mechanisms such as dual-atom active sites and substrate-surface interactions. Key applications include disease biomarker detection, medical imaging enhancement, and point-of-care diagnostics aligned with the ASSURED criteria. In clinical contexts, advances such as wearable biosensors and smart diagnostic platforms leverage DL for real-time signal processing, pattern recognition, and adaptive decision-making. Despite significant progress, challenges remain—particularly the need for standardized biomedical datasets, improved model robustness across diverse populations, and the clinical translation of artificial intelligence (AI)-enhanced nanozyme systems. Future directions include integration with the Internet of Medical Things, personalized medicine frameworks, and sustainable sensor development. The convergence of nanozymes and DL offers unprecedented opportunities to advance intelligent biosensing and reshape precision diagnostics in healthcare. Full article
(This article belongs to the Special Issue Cutting-Edge Nanozyme Biosensing Strategies for Biomedical and Environmental Applications)
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Graphical abstract

13 pages, 1289 KB  
Review
Peroxidase-Mimicking Nanozymes of Nitrogen Heteroatom-Containing Graphene Oxide for Biomedical Applications
by Phan Gia Le, Daesoo Kim, Jae-Pil Chung and Sungbo Cho
Biosensors 2025, 15(7), 435; https://doi.org/10.3390/bios15070435 - 7 Jul 2025
Viewed by 813
Abstract
Nanozymes constitute a rapidly advancing frontier in scientific research, attracting widespread international interest, particularly for their role in facilitating cascade reactions. Despite their initial discovery a few years ago, significant hurdles persist in optimizing their catalytic performance and substrate specificity—challenges that are especially [...] Read more.
Nanozymes constitute a rapidly advancing frontier in scientific research, attracting widespread international interest, particularly for their role in facilitating cascade reactions. Despite their initial discovery a few years ago, significant hurdles persist in optimizing their catalytic performance and substrate specificity—challenges that are especially critical in the context of biomedical diagnostics. Within this domain, nitrogen-containing graphene oxide-based nanozymes exhibiting peroxidase-mimicking activity have emerged as particularly promising candidates, owing to the exceptional electrical conductivity, mechanical flexibility, and structural resilience of reduced graphene oxide-based materials. Intensive efforts have been devoted to engineering graphene oxide structures to enhance their peroxidase-like functionality. Nonetheless, the practical implementation of such nanozymes remains under active investigation and demands further refinement. This review synthesizes the current developments in nitrogen heteroatom-containing graphene oxide nanozymes and their derivative nanozymes, emphasizing recent breakthroughs and biomedical applications. It concludes by exploring prospective directions and the broader potential of these materials in the biomedical landscape. Full article
(This article belongs to the Special Issue Cutting-Edge Nanozyme Biosensing Strategies for Biomedical and Environmental Applications)
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Other

Jump to: Research, Review

44 pages, 8360 KB  
Systematic Review
Natural Iron Oxide Nanoparticles Produced by Aquatic Magnetotactic Bacteria as Ideal Nanozymes for Nano-Guided Biosensing Platforms—A Systematic Review
by Natalia Lorela Paul, Catalin Ovidiu Popa and Rodica Elena Ionescu
Biosensors 2025, 15(9), 590; https://doi.org/10.3390/bios15090590 - 8 Sep 2025
Viewed by 693
Abstract
In response to the ongoing challenges associated with natural enzymes, their high production costs, low stability and limited functionality; nanozymes have rapidly emerged as versatile alternative. Such nanocatalysts, based on nanomaterials and nanostructures, offer remarkable tunability of physicochemical properties and excellent durability, and [...] Read more.
In response to the ongoing challenges associated with natural enzymes, their high production costs, low stability and limited functionality; nanozymes have rapidly emerged as versatile alternative. Such nanocatalysts, based on nanomaterials and nanostructures, offer remarkable tunability of physicochemical properties and excellent durability, and adapt themselves effectively to the requirements of modern biotechnological applications. This review article aims to provide a comprehensive overview of recent advances in the use of naturally occurring iron oxide nanoparticles, produced by magnetotactic bacteria, and to highlight their emerging role as key elements in the development of the new generation of nano-guided biosensors. It provides a comprehensive and systematic analysis of publications in the Web of Science database between 2022 and August 2025, conducted in accordance with PRISMA guidelines. The aim was to assess the current state of the art and identify knowledge gaps in the exploration and application of magnetotactic bacteria as natural and sustainable sources in the design of next-generation biosensors. The natural nanoparticles, formed through biological processes, represent a unique and sustainable alternative to synthetic nanoparticles, offering naturally mimetic enzymatic activity, high biocompatibility, and exceptional stability. This approach opens up revolutionary perspectives in the field of biosensors, proposing a new class of functional materials, iron nanoparticles of biological origin, capable of fundamentally changing the performance, sustainability and reliability of future nanoenzymatic sensing platforms. Full article
(This article belongs to the Special Issue Cutting-Edge Nanozyme Biosensing Strategies for Biomedical and Environmental Applications)
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