Nano Biosensors and Their Applications for In Vivo/Vitro Diagnosis—3rd Edition

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor Materials".

Deadline for manuscript submissions: 15 June 2026 | Viewed by 636

Special Issue Editor


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Guest Editor
Marshall Laboratory of Biomedical Engineering, Research Center for Biosensor and Nanotheranostic, School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen 518060, China
Interests: chemical and biological sensors; optical sensors; metal nanomaterials; catalysis; plasmonics; microfluidics; disease diagnosis; environmental monitoring
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Special Issue Information

Dear Colleagues,

Following the successful publication of the first and second editions of the Special Issue entitled “Nano-Biosensors and Their Applications for In Vivo/Vitro Diagnosis”, we are pleased to launch the third volume of this ongoing series. The previous editions have received broad attention and have significantly advanced the field of nano-biosensing. Building on this foundation, the third edition aims to further explore nanotechnology-enabled diagnostics, with renewed focus on innovation, translational potential, and interdisciplinary integration.

Nano-biosensors represent a distinctive class of analytical tools that integrate the molecular recognition capabilities of biomolecules (such as antibodies, aptamers, nucleic acids, and enzymes) with the unique properties of nanostructures (such as optical, catalytic, magnetic, and plasmonic features). This powerful synergy offers superior sensitivity, specificity, and versatility for the detection of biological targets in both in vitro and in vivo settings.

In recent years, we have witnessed exciting developments in the field, including the emergence of digital and wearable biosensors, artificial intelligence-assisted signal processing, and multiplexed detection platforms tailored for clinical applications. These advances have expanded the scope of nano-biosensing beyond fundamental research toward practical implementation in disease diagnosis, health monitoring, and personalized medicine.

This Special Issue welcomes original research and review articles focused on the design of novel nanostructures, the development of high-performance biosensors, innovative signal amplification mechanisms, and the detection of emerging biomarkers. Of particular interest are studies involving point-of-care diagnostics, implantable and wearable devices, and intelligent biosensing systems powered by artificial intelligence.

We look forward to your contributions that will further shape this rapidly evolving and multidisciplinary research area.

Dr. Zhuangqiang Gao
Guest Editor

Manuscript Submission Information

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Keywords

  • nanostructures
  • nanomaterials
  • nanodevices
  • biosensors
  • biomarkers
  • artificial intelligence
  • in vivo/vitro diagnosis

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Published Papers (1 paper)

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Review

19 pages, 1908 KiB  
Review
Enhancing ELISA Sensitivity: From Surface Engineering to Synthetic Biology
by Hye-Bin Jeon, Dong-Yeon Song, Yu Jin Park and Dong-Myung Kim
Biosensors 2025, 15(7), 434; https://doi.org/10.3390/bios15070434 - 6 Jul 2025
Viewed by 569
Abstract
Accurate and sensitive detection of protein biomarkers is critical for advancing in vitro diagnostics (IVD), yet conventional enzyme-linked immunosorbent assays (ELISA) often fall short in terms of sensitivity compared to nucleic acid-based tests. Bridging this sensitivity gap is essential for improving diagnostic accuracy, [...] Read more.
Accurate and sensitive detection of protein biomarkers is critical for advancing in vitro diagnostics (IVD), yet conventional enzyme-linked immunosorbent assays (ELISA) often fall short in terms of sensitivity compared to nucleic acid-based tests. Bridging this sensitivity gap is essential for improving diagnostic accuracy, particularly in diseases where protein levels better reflect disease progression than nucleic acid biomarkers. In this review, we present strategies developed to enhance the sensitivity of ELISA, structured according to the sequential steps of the assay workflow. Beginning with surface modifications, we then discuss the methodologies to improve mixing and washing efficiency, followed by a summary of recent advances in signal generation and amplification techniques. In particular, we highlight the emerging role of cell-free synthetic biology in augmenting ELISA sensitivity. Recent developments such as expression immunoassays, CRISPR-linked immunoassays (CLISA), and T7 RNA polymerase–linked immunosensing assays (TLISA) demonstrate how programmable nucleic acid and protein synthesis systems can be integrated into ELISA workflows to surpass the present sensitivity, affordability, and accessibility. By combining synthetic biology-driven amplification and signal generation mechanisms with traditional immunoassay formats, ELISA is poised to evolve into a highly modular and adaptable diagnostic platform, representing a significant step toward the next generation of highly sensitive and programmable immunoassays. Full article
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