Molecular Regulation of Spermatozoa—Second Edition

A special issue of Biomedicines (ISSN 2227-9059). This special issue belongs to the section "Cell Biology and Pathology".

Deadline for manuscript submissions: 30 September 2025 | Viewed by 401

Special Issue Editor


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Guest Editor
Department of Biology, University of Saskatchewan, Saskatoon, SK S7N 5E2, Canada
Interests: reproduction; female fertility preservation; male fertility preservation; female infertility; male infertility; oocyte; sperm; embryo; ovarian tissue; spermatogonial stem cell; testicular tissue; reproductive cells and tissues culture; reproductive cells and tissues cryopreservation; apoptosis; oxidative stress; antioxidant therapy
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Dear Colleagues,

Infertility is a common problem affecting many couples worldwide. In approximately 50% of cases, the male factor is the primary or contributing cause. In most infertile cases, adequate numbers of spermatozoa are produced, and in fact, these numbers are sufficient to induce pregnancy; however, the biological functions such as capacitation, hyperactivation, acrosome reaction, and fertilizing ability of those spermatozoa have been compromised and are considered the most frequent cause of human infertility. A dynamic interplay between spermatozoa and a constantly changing environment is necessary to activate the underlying molecular events and signaling pathways that regulate sperm functions. Various signaling molecules, such as hormones, immune regulatory molecules, neurotransmitters, ions, proteins, growth factors, and free radicals, regulate sperm functions, mainly through post-translational modifications of proteins. Therefore, the deregulation of those interactions can be behind male factor infertility.

For this Special Issue of ”Biomedicines”, we invite you to contribute either an original research article or a review article on the topic “Molecular Regulation of Spermatozoa.” The scope includes in vitro and in vivo research on molecular mechanisms and signaling pathways that regulate spermatozoa function in physiological conditions and under special conditions, such as those causing male infertility or subfertility. Additionally, any paper related to the redox regulation role in sperm function is welcome.

Dr. Atefeh Najafi
Guest Editor

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Keywords

  • spermatogenesis
  • spermiogenesis
  • sperm
  • capacitation
  • acrosome reaction
  • hyperactivation
  • fertilization
  • male infertility
  • antioxidants
  • redox regulation

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Published Papers (1 paper)

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Research

15 pages, 914 KiB  
Article
A Comprehensive Comparison of PICSI and ICSI Techniques Through a Triple-Blinded Trial: Effects on Embryo Quality, Cumulative Pregnancy Rate, and Live Birth Rate
by Lucia Alegre, Laura Carrión-Sisternas, Lorena Bori, Irene Hervás, Jose Remohí, Nicolás Garrido and Marcos Meseguer
Biomedicines 2025, 13(5), 1104; https://doi.org/10.3390/biomedicines13051104 - 1 May 2025
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Abstract
Background: Sperm selection is critical in assisted reproduction, typically relying on swim-up and centrifugation density gradients. New methods, such as PICSI (physiological intracytoplasmic sperm selection), aim to enhance outcomes by selecting mature sperm based on hyaluronic acid (HA) binding and have generated [...] Read more.
Background: Sperm selection is critical in assisted reproduction, typically relying on swim-up and centrifugation density gradients. New methods, such as PICSI (physiological intracytoplasmic sperm selection), aim to enhance outcomes by selecting mature sperm based on hyaluronic acid (HA) binding and have generated interest due to their potential impact on the clinical outcomes of patients who undergo assisted reproductive treatments. Methods: A single-center, prospective, and triple-blinded study was conducted with 277 couples in the egg donation program. The oocytes of each recipient patient were randomly microinjected using the ICSI or PICSI technique and maintained in culture in time-lapse incubators until blastocyst formation. Biological and clinical outcomes were analyzed, including fertilization and blastocyst formation rates, embryo morphokinetics, pregnancy, miscarriage, and live birth rates, and artificial intelligence-assigned embryo quality scores. Results: Clinical outcomes were comparable between the two groups, but a higher pregnancy rate was observed in the PICSI group than in the ICSI group (74.04% vs. 70.87%). Although blastocyst formation rates were similar on both day 5 (D5) and day 6 of development, the proportion of good-quality embryos on D5 was higher in the PICSI group (68.27%) than in the ICSI group (63.47%) (p > 0.05). Finally, the cumulative pregnancy rate was significantly higher in the experimental group than in the control group (88% vs. 72%) after four embryo transfers (p < 0.05). Conclusions: Utilizing HA to perform sperm selection during ICSI procedures does not increase live birth rates. However, it may enhance the quality of the selected sperm. This could be beneficial for patients in egg donation programs, particularly for those who have experienced repeated pregnancy loss. Full article
(This article belongs to the Special Issue Molecular Regulation of Spermatozoa—Second Edition)
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