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Applied Sciences
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Applied Microbial Biotechnology for Poultry Science, 2nd Edition
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Applied Microbial Biotechnology for Poultry Science, 2nd Edition

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Applied Microbiology".

Deadline for manuscript submissions: 20 November 2025 | Viewed by 1414

Special Issue Editors

Dr. Elena George Olson

E-Mail Website
Guest Editor
Meat Science and Animal Biologics Discovery Program, Department of Animal and Dairy Sciences, University of Wisconsin, Madison, WI 53706, USA
Interests: microbiome dynamics; meat microbiology; poultry science; foodborne pathogens; Campylobacter
Special Issues, Collections and Topics in MDPI journals
Dr. Dana Kristen Dittoe

E-Mail Website
Guest Editor
Department of Animal Science, University of Wyoming, Laramie, WY 82071, USA
Interests: meat microbiology; pre- and post-harvest food safety; poultry science; microbial ecology; shelf-life extension
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

We are inviting submissions for the Special Issue “Applied Microbial Biotechnology for Poultry Science, 2nd Edition”.

Microbial biotechnology has emerged as a critical tool to drive innovation in poultry production. This approach utilizes the beneficial properties of bacteria to enhance birds' health and performance. Microbial biotechnology is essential in creating a more sustainable and efficient poultry production system by promoting digestion, improving immune function, and preventing pathogen proliferation.

This Special Issue seeks to collect the most up-to-date information on advancements in the direct application of microbial biotechnology throughout the poultry industry while addressing the impact on health, disease, and performance. These topics include but are not limited to novel vaccine developments that provide a more effective method than conventional vaccines; probiotics and feed additives that improve gastrointestinal (GIT) function, prevent pathogen proliferation, and enhance performance; strategies that focus on fermented feed and enzyme production to increase nutrient digestibility and microbial diversity in poultry GIT; application of bacteriophages and antimicrobial peptides that can be used as alternatives to traditional antibiotics; lastly, characterization of potential biocontrol agents that can be utilized as post-harvest disinfectants and may reduce chemical interventions.

Ultimately, this Special Issue will address the use of microbial biotechnology to advance food safety efforts during the pre-and post-harvest stages of poultry, providing insight into how microbial biotechnology can shape the future of poultry production.

Created in BioRender. Olson, E. (2024) https://BioRender.com/c52d475.

Dr. Elena George Olson
Dr. Dana Kristen Dittoe
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • microbiome
  • microbial biotechnology
  • nutrition
  • food safety
  • broilers
  • turkeys
  • laying hens

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Related Special Issue

Published Papers (2 papers)

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Research

19 pages, 1903 KB  
Article
Ligilactobacillus salivarius UMNPBX2 Cell-Free Extract Has Antiviral Effects on H4N6 Low-Pathogenic Avian Influenza Virus Subtype in Madin–Darby Canine Kidney Cell Line and Embryonated Chicken Eggs
by Amritha Ajayan, Dhananjai Muringattu Prabhakaran, Venkatramana Divana Krishna, Maxim C.-J. Cheeran and Anup Kollanoor Johny
Appl. Sci. 2025, 15(18), 10075; https://doi.org/10.3390/app151810075 - 15 Sep 2025
Viewed by 397
Abstract
Avian influenza (AI) is a highly infectious disease affecting birds. Some strains of AI virus (AIV) have zoonotic potential, posing a threat to humans. The H4N6 subtype is a low-pathogenic virus and causes mild infection in poultry. However, it has raised increasing concern [...] Read more.
Avian influenza (AI) is a highly infectious disease affecting birds. Some strains of AI virus (AIV) have zoonotic potential, posing a threat to humans. The H4N6 subtype is a low-pathogenic virus and causes mild infection in poultry. However, it has raised increasing concern due to its capability to infect pigs and its high potential for reassortment when co-infected with other strains. This study investigated the antiviral properties of turkey-derived Ligilactobacillus salivarius UMNPBX2 (L. salivarius UMNPBX2) cell-free extract (CFE) using both cell culture and in ovo methods. We assessed the growth kinetics of the H4N6 virus and the cytotoxicity of L. salivarius UMNPBX2 CFE in Madin–Darby Canine Kidney (MDCK) cells. The results revealed that the CFE from the 109 CFU/mL L. salivarius UMNPBX2 overnight culture had strong antiviral activities (p < 0.05). The CFE obtained from 107 to 105 CFU/mL of overnight culture also significantly reduced viral replication (p < 0.05), demonstrating dose-dependent inhibition of viral replication. Additionally, CFEs did not increase pro-inflammatory cytokine gene expression of IL-1β and IL-6 but rather tended to decrease it (IL-6). The embryo survivability experiments revealed a significant dose-dependent increase in survival rate (p < 0.05). The findings of this study highlight the antiviral properties of L. salivarius UMNPBX2 CFE, which contain potential postbiotics against the H4N6 virus, warranting in vivo studies. Full article
(This article belongs to the Special Issue Applied Microbial Biotechnology for Poultry Science, 2nd Edition)
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17 pages, 871 KB  
Article
Evaluating the Role of Canada Goose Populations in Transmission Dynamics During Peak HPAI Incidence in Iowa, February 2022–December 2023
by Christopher Jimenez, Sergios-Orestis Kolokotronis, Janet E. Rosenbaum and Lori A. Hoepner
Appl. Sci. 2025, 15(12), 6900; https://doi.org/10.3390/app15126900 - 19 Jun 2025
Viewed by 565
Abstract
Since its emergence in the United States in February 2022, Highly Pathogenic Avian Influenza (HPAI) H5N1 has caused significant losses for poultry operations, particularly in Iowa between February 2022 and December 2023. Branta canadensis (Canada goose), an abundant North American waterfowl species, is [...] Read more.
Since its emergence in the United States in February 2022, Highly Pathogenic Avian Influenza (HPAI) H5N1 has caused significant losses for poultry operations, particularly in Iowa between February 2022 and December 2023. Branta canadensis (Canada goose), an abundant North American waterfowl species, is considered a potential reservoir host for H5N1. This study examined the relationship between Canada goose abundance and H5N1 occurrence in Iowa counties. Although counties with H5N1 cases comprised 13% of the state’s Canada goose population—and 32% of those counties had high goose abundance—an inverse relationship was observed. Bivariate analysis indicated that counties with high goose abundance were significantly less likely to report HPAI cases (χ2 = 4.29, p = 0.04). Notably, intermediate goose abundance was associated with a 79% lower likelihood of HPAI occurrence (RR = 0.21, 95% CI [0.05, 0.90], p = 0.04). These findings highlight the limitations posed by the lack of accessible, high-resolution poultry farm location data, which hinders a definitive understanding of Canada geese’s role in H5N1 transmission. To address this gap, stakeholders should consider adopting next-generation surveillance tools like the Biothreats Emergence Analysis and Communication Network (BEACON) AI platform, or AI-integrated chemical sensors that generate real-time, actionable data for biosecurity decision-making. Given the uncertainty surrounding Canada goose role transmission dynamics, the species remains a relevant One Health concern. Full article
(This article belongs to the Special Issue Applied Microbial Biotechnology for Poultry Science, 2nd Edition)
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