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Advances in Functional Properties of Probiotics

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Applied Biosciences and Bioengineering".

Deadline for manuscript submissions: 20 January 2026 | Viewed by 448

Special Issue Editors


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Guest Editor
Department of Pharmacobiology, Universidad de Guadalajara, Guadalajara 44100, Mexico
Interests: functional foods; probiotics; prebiotics; human milk; lactic acid bacteria; yeasts; food safety; applied microbiology

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Guest Editor
Department of Pharmacobiology, Universidad de Guadalajara, Guadalajara 44100, Mexico
Interests: applied microbiology; enzymatic catalysis; bioreactors; probiotics; yeasts

E-Mail Website
Guest Editor
Department of Pharmacobiology, Universidad de Guadalajara, Guadalajara 44100, Mexico
Interests: food microbiology; functional foods
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Special Issue Information

Dear Colleagues,

Probiotics are live microorganisms that, when ingested, inhabit the intestine, helping to digest food, produce vitamins, and even destroy disease-causing cells. In the pharmaceutical and translational medicine field, new alternatives to medications are being sought for the prevention and therapeutic treatment of intestinal disorders, urogenital infections, and degenerative diseases. Probiotics, along with other bioactive molecules such as prebiotics and antioxidants (polyphenols, tocopherols, vitamins), are an excellent preventive alternative. Findings in recent years demonstrate that the physiological and clinical actions of probiotics with specific strains are becoming increasingly relevant in the development of functional foods associated with probiotics. Thanks to their function and biological activity, improving digestive health and strengthening the immune system, the probiotics have become priority ingredients in the production of functional foods for human and animal nutrition. Therefore, the general objective of this Special Issue is to contribute to innovative research in this area of ​​knowledge, promoting the exploration and discovery of new strains with probiotic potential, ensuring their biological function through biological and immunological studies at the cellular level "in vitro" and "in vivo" that guarantee the application of probiotics in the areas of medicine, pharmacy and food, thus contributing to human and animal health.

Dr. Blanca Rosa Aguilar Uscanga
Dr. Josué Raymundo Solis Pacheco
Dr. Angélica Villarruel-López
Guest Editors

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Keywords

  • probiotics
  • prebiotics
  • functional foods
  • lactic acid bacteria
  • yeasts
  • human milk
  • dairy products
  • biological activity
  • functional properties

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Published Papers (1 paper)

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16 pages, 2243 KiB  
Case Report
Lactiplantibacillus sp. LH01 as an Adjuvant to Reduce Antibiotic Use in Recurrent Urinary Tract Infections in a Paediatric Patient with Hydronephrosis
by Naomi Aguirre Hernández, Daniel Pérez-Rulfo Ibarra, Blanca Rosa Aguilar Uscanga, Elisa García Morales, Ixtlilxochitl Flores Fong and Jesús Alonso Amezcua López
Appl. Sci. 2025, 15(16), 8805; https://doi.org/10.3390/app15168805 - 9 Aug 2025
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Abstract
Background: Urinary tract infections (UTIs) represent a significant public health concern, particularly in children with structural abnormalities such as vesicoureteric reflux. Prolonged antibiotic exposure in these patients often contributes to the emergence of multidrug-resistant organisms and restricts therapeutic options. Probiotics have emerged as [...] Read more.
Background: Urinary tract infections (UTIs) represent a significant public health concern, particularly in children with structural abnormalities such as vesicoureteric reflux. Prolonged antibiotic exposure in these patients often contributes to the emergence of multidrug-resistant organisms and restricts therapeutic options. Probiotics have emerged as a potential adjuvant strategy to reduce infection recurrence. Case Presentation: A female infant born at term (38.6 weeks), with a prenatal diagnosis of bilateral hydronephrosis, experienced recurrent UTIs from the neonatal period despite both prophylactic and therapeutic antibiotic regimens. Serial urine cultures revealed infections caused by extended-spectrum beta-lactamase (ESBL)-producing E. coli and K. pneumoniae. Methods: The isolated strains were evaluated in vitro against Lactiplantibacillus sp. LH01, a probiotic strain derived from human milk. Following confirmation of its antimicrobial activity, an individualised intervention was initiated: daily oral administration of 1 mL of the probiotic (109 CFU/mL) for one month, under medical supervision and without concurrent antibiotic therapy. Results: The probiotic demonstrated 89% inhibition efficiency against multidrug-resistant strains, accompanied by a notable reduction in UTIs frequency. Follow-up cultures showed reduced pathogen growth and a loss of the ESBL phenotype, facilitating clinical management and allowing previously contraindicated surgical interventions. Conclusions: Lactiplantibacillus sp. LH01 proved a safe and effective adjuvant in managing recurrent, resistant UTIs in a paediatric patient, highlighting the promise of probiotic therapies. Full article
(This article belongs to the Special Issue Advances in Functional Properties of Probiotics)
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