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Open AccessCase Report
Lactiplantibacillus sp. LH01 as an Adjuvant to Reduce Antibiotic Use in Recurrent Urinary Tract Infections in a Paediatric Patient with Hydronephrosis
by
Naomi Aguirre Hernández
Naomi Aguirre Hernández
Miss Naomi Aguirre Hernández is a licensed Pharmaceutical Chemist Biologist with a Bachelor’s and [...]
Miss Naomi Aguirre Hernández is a licensed Pharmaceutical Chemist Biologist with a Bachelor’s degree from UTEG University and a technical qualification as a Food Chemistry Technician from the Escuela Politécnica de Guadalajara. She is currently undertaking a Doctorate in Sciences and Biotechnological Processes at the University of Guadalajara, CUCEI campus. Throughout her academic and professional trajectory, she has contributed to research projects focusing on the nutritional and microbiological assessment of human milk from mothers recovered from COVID-19, acquiring substantial expertise in bromatological and microbiological analyses, molecular biology, and clinical diagnostics. Her professional experience includes positions as a Clinical Analyst at Ampharma, Quality Control Assistant, and responsibilities in medication management and patient care at the Hospital de Padua. Ms Aguirre Hernández also has practical experience in food production inspection and quality control, complemented by training in occupational safety, first aid, and the HACCP system. Her multidisciplinary profile reflects a strong commitment to scientific research, rigorous laboratory practices, and ongoing professional development within the fields of clinical and biotechnological sciences.
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Daniel Pérez-Rulfo Ibarra
Daniel Pérez-Rulfo Ibarra
Dr. Daniel Pérez Rulfo Ibarra is a Pediatric Neurologist and Professor with extensive academic and [...]
Dr. Daniel Pérez Rulfo Ibarra is a Pediatric Neurologist and Professor with extensive academic and clinical experience. He holds a Medical Degree from the University of Guadalajara, a Specialty in Pediatrics from the National Autonomous University of Mexico (UNAM), and a Subspecialty in Pediatric Neurology from the National Institute of Pediatrics. He also holds a Master’s Degree in Family Sciences from Anahuac University and a Doctorate in Administration from Instituto Hispano. He is currently pursuing a Doctorate in Educational Competencies.
Dr. Pérez Rulfo Ibarra currently serves as a Professor B at the University of Guadalajara and as the Head Professor of the Pediatrics Specialty Program at the Civil Hospital of Guadalajara “Fray Antonio Alcalde.”
He is a full academic member and active fellow of the Mexican Academy of Pediatrics (since 2017), as well as an active member of the Mexican Academy of Neurology and the Mexican Society of Pediatric Neurology. He serves as a Board Member of the Mexican Council of Neurology and has been part of the Research Ethics Committee at the Civil Hospital of Guadalajara since August 2019.
In 2022, he was recognized by the Mexican National Council of Science and Technology (CONAHCYT) as a National Researcher, Level 1 (SNI 1). His work promotes the integration of scientific knowledge with national priorities, focusing on humanistic, socially committed research that benefits the wellbeing of the population.
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Blanca Rosa Aguilar Uscanga
Blanca Rosa Aguilar Uscanga
Doctor of Science in Biotechnology, graduate of the Institut National des Sciences Appliquées in of [...]
Doctor of Science in Biotechnology, graduate of the Institut National des Sciences Appliquées in Toulouse, France. Master of Food Science and Biochemical Engineer specializing in Food. Currently a full-time Research Professor at the University Center for Exact Sciences and Engineering at Universidad de Guadalajara in México. Member of the National System of Researchers (SNI) with a level 3 designation. Honorary Associate Professor at INRS-Institute Armand Frappier in Canada. Evaluator for CONACYT, PRODEP, and reviewer for scientific articles in various international journals. Currently serving as the President of the International Food Sciences Congress of the Common Ground International Network for Nutritional Studies. The research areas she focuses on include Food Biotechnology, development of functional foods such as human milk, probiotics production, microbial metabolites, and food safety.
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Elisa García Morales
Elisa García Morales
Pediatrician and neonatologist, with a master's degree in human nutrition focused on maternal and of [...]
Pediatrician and neonatologist, with a master's degree in human nutrition focused on maternal and child health, professor of subject "B" at the School of Medicine, and coordinator of the Neonatology specialty at the Fray Antonio Alcalde Civil Hospital of Guadalajara, Department of Human Reproduction Clinics, Child Growth and Development, University Center for Health Sciences, University of Guadalajara, Guadalajara, Jalisco, Mexico. Research interests include healthy newborns and sick newborns.
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Ixtlilxochitl Flores Fong
Ixtlilxochitl Flores Fong
Mtra. Ixtlilxochitl Flores Fong holds a Bachelor’s Degree in Nutrition from the University Center [...]
Mtra. Ixtlilxochitl Flores Fong holds a Bachelor’s Degree in Nutrition from the University Center for Health Sciences (CUCS) at the University of Guadalajara, a Master’s Degree in Clinical Nutrition from the Universidad del Valle de México, and is currently pursuing a Ph.D. in Microbiology and Molecular Biotechnology at the University Center for Exact Sciences and Engineering of the University of Guadalajara. She is a Certified Nutritionist by the Mexican College of Nutritionists and currently serves as Treasurer of the Jalisco Chapter Board of Directors of the same institution. She is a Full-Time Associate Professor “A” at CUCS, where she also serves as the Coordinator of the Bachelor’s Degree in Nutrition. Additionally, she coordinates the Technical Support Group for the Curriculum Restructuring of the same program and is a member of various technical committees focused on educational quality and breastfeeding. She is the author of book chapters on nutrition and food sovereignty. In 2025, she received the “Outstanding Nutritionist” Recognition from the Jalisco Ministry of Health.
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and
Jesús Alonso Amezcua López
Jesús Alonso Amezcua López
Dr Jesús Alonso Amezcua López holds a PhD in Biotechnological Processes and a degree in Food and a [...]
Dr Jesús Alonso Amezcua López holds a PhD in Biotechnological Processes and a degree in Food Engineering and Biotechnology from the Universidad de Guadalajara. He is currently a faculty member and researcher at Centro Universitario UTEG, where he leads the research line in Food Biotechnology and New Product Development, and serves as Head of the Academy of Methodological Disciplines.
His research focuses on nutrition, biotechnology, and molecular biology, with an emphasis on nutrigenetics and nutrigenomics. He has conducted significant studies on powdered human milk, its fortification with probiotics, and its impact on the nutrition of premature and critically ill neonates. He also investigates the effects of maternal consumption of toxic substances during lactation and their influence on neonatal microbiota and development.
Dr Amezcua has published in indexed journals, presented at national and international conferences, and received recognitions including an honourable mention and the 2023 Latin American Innovative Idea Award.
He collaborates with the Hospital Civil “Fray Antonio Alcalde” , the Human Milk Research Laboratory at CUCEI/UDG, and maintains a partnership with Casa Hogar Cabañas. Additionally, he promotes public health policies aimed at improving neonatal nutrition in vulnerable populations.
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Human Milk Research Laboratory, Department of Pharmacology, University Center of Exact Sciences and Engineering, Universidad de Guadalajara, Guadalajara 44430, Jalisco, Mexico
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Child Growth and Development, Paediatrics Speciality, Department of Human Reproduction Clinics, University Health Sciences Centre, Universidad de Guadalajara, Guadalajara 44340, Jalisco, Mexico
3
Neonatology Unit, Division of Paediatrics, Department of Human Reproduction Clinics, Growth and Child Development, O.P.D. Hospital Civil de Guadalajara Fray Antonio Alcalde/Neonatology Specialisation, University Centre for Health Sciences, Universidad def Guadalajara, Guadalajara 44200, Jalisco, Mexico
4
Department of Food and Nutrition, University Health Sciences Centre, Universidad de Guadalajara, Guadalajara 44340, Jalisco, Mexico
5
BSc in Nutrition, Academy of Biochemistry, Genetics and Food Technology, Centro Universitario UTEG, Health Sciences Campus, Guadalajara 44460, Jalisco, Mexico
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Authors to whom correspondence should be addressed.
Appl. Sci. 2025, 15(16), 8805; https://doi.org/10.3390/app15168805 (registering DOI)
Submission received: 5 July 2025
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Revised: 6 August 2025
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Accepted: 7 August 2025
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Published: 9 August 2025
Featured Application
Lactiplantibacillus sp. LH01 may be used as an adjuvant to reduce antibiotic use in paediatric patients with recurrent urinary tract infections, thereby helping to prevent antimicrobial resistance.
Abstract
Background: Urinary tract infections (UTIs) represent a significant public health concern, particularly in children with structural abnormalities such as vesicoureteric reflux. Prolonged antibiotic exposure in these patients often contributes to the emergence of multidrug-resistant organisms and restricts therapeutic options. Probiotics have emerged as a potential adjuvant strategy to reduce infection recurrence. Case Presentation: A female infant born at term (38.6 weeks), with a prenatal diagnosis of bilateral hydronephrosis, experienced recurrent UTIs from the neonatal period despite both prophylactic and therapeutic antibiotic regimens. Serial urine cultures revealed infections caused by extended-spectrum beta-lactamase (ESBL)-producing E. coli and K. pneumoniae. Methods: The isolated strains were evaluated in vitro against Lactiplantibacillus sp. LH01, a probiotic strain derived from human milk. Following confirmation of its antimicrobial activity, an individualised intervention was initiated: daily oral administration of 1 mL of the probiotic (109 CFU/mL) for one month, under medical supervision and without concurrent antibiotic therapy. Results: The probiotic demonstrated 89% inhibition efficiency against multidrug-resistant strains, accompanied by a notable reduction in UTIs frequency. Follow-up cultures showed reduced pathogen growth and a loss of the ESBL phenotype, facilitating clinical management and allowing previously contraindicated surgical interventions. Conclusions: Lactiplantibacillus sp. LH01 proved a safe and effective adjuvant in managing recurrent, resistant UTIs in a paediatric patient, highlighting the promise of probiotic therapies.
Share and Cite
MDPI and ACS Style
Aguirre Hernández, N.; Pérez-Rulfo Ibarra, D.; Aguilar Uscanga, B.R.; García Morales, E.; Flores Fong, I.; Amezcua López, J.A.
Lactiplantibacillus sp. LH01 as an Adjuvant to Reduce Antibiotic Use in Recurrent Urinary Tract Infections in a Paediatric Patient with Hydronephrosis. Appl. Sci. 2025, 15, 8805.
https://doi.org/10.3390/app15168805
AMA Style
Aguirre Hernández N, Pérez-Rulfo Ibarra D, Aguilar Uscanga BR, García Morales E, Flores Fong I, Amezcua López JA.
Lactiplantibacillus sp. LH01 as an Adjuvant to Reduce Antibiotic Use in Recurrent Urinary Tract Infections in a Paediatric Patient with Hydronephrosis. Applied Sciences. 2025; 15(16):8805.
https://doi.org/10.3390/app15168805
Chicago/Turabian Style
Aguirre Hernández, Naomi, Daniel Pérez-Rulfo Ibarra, Blanca Rosa Aguilar Uscanga, Elisa García Morales, Ixtlilxochitl Flores Fong, and Jesús Alonso Amezcua López.
2025. "Lactiplantibacillus sp. LH01 as an Adjuvant to Reduce Antibiotic Use in Recurrent Urinary Tract Infections in a Paediatric Patient with Hydronephrosis" Applied Sciences 15, no. 16: 8805.
https://doi.org/10.3390/app15168805
APA Style
Aguirre Hernández, N., Pérez-Rulfo Ibarra, D., Aguilar Uscanga, B. R., García Morales, E., Flores Fong, I., & Amezcua López, J. A.
(2025). Lactiplantibacillus sp. LH01 as an Adjuvant to Reduce Antibiotic Use in Recurrent Urinary Tract Infections in a Paediatric Patient with Hydronephrosis. Applied Sciences, 15(16), 8805.
https://doi.org/10.3390/app15168805
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