Special Issue "Current Contribution to the Research Based on Animal Tissue and Cellular Models"

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Animal Physiology".

Deadline for manuscript submissions: 31 May 2021.

Special Issue Editors

Dr. Katarzyna Stadnicka
Guest Editor
UTP University of Science and Technology in Bydgoszcz, Poland, Faculty of Animal Breeding and Biology
Interests: In vitro models, establishment of avian cell cultures (oviduct, primordial germ cells); and advances in cell analysis, secretome, and proteomics; In ovo technology to shape the gut health and resistance (early, prenatal delivery of bioactive compounds); testing natural alternatives to microbials in poultry production
Dr. Luiz Cordeiro
Guest Editor
Cell Interactions and Fertility team, PRC, INRAE, Nouzilly, France
Interests: oviduct secretome; sperm-oocyte interactions and oviduct-embryo cross-talk; development of in vitro models to study oviduct physiology

Special Issue Information

Data from animal (livestock) trials are intricate and influenced by environmental factors, as well as by individual animal biology. Often, the straightforward interpretation of the results is hardly possible. Thus, simplified biological systems have become an inherent component of animal experimental research.

Cell/organoid-based research in livestock science only seems to be thriving for the past decade. The knowledge of emerging and newly established cell model concepts may be greatly expected and applicable in the field.

This Special Issue will collect and publish original research, short communications, and review articles that will disseminate in vitro studies of livestock species (including but not limited to poultry and pigs). We believe that the proposed compilation of publications will improve our understanding of certain physiological, genetic, and metabolic processes, but will also highlight the most recent cellular techniques that might be adapted in the laboratories.

The manuscripts addressing, but not limited to the following topics are welcome:

  1. Investigating molecular mechanisms of biological interactions in the organism using in vitro models (e.g., microbiome–host interactions, environmental impact, secretory functions in tissues)
  2. Role of tissue microenvironments in development (e.g., germplasm maturation, epigenetic impact, and programming)
  3. Studies based on organ and cell models, including new cell cultures and cell lines, mini organoids, organs on-chip, and microfluidic culture systems and cellular techniques and molecular analyses adapted to livestock research: visualization techniques, omic analyses

Dr. Katarzyna Stadnicka
Dr. Luiz Cordeiro
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Animals is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • Cell culture
  • mini-organs
  • 3D culture
  • organ-on-chip
  • microfluidic culture system
  • cellular techniques
  • cell omics

Published Papers (1 paper)

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Open AccessArticle
In Vitro Culture of Chicken Circulating and Gonadal Primordial Germ Cells on a Somatic Feeder Layer of Avian Origin
Animals 2020, 10(10), 1769; https://doi.org/10.3390/ani10101769 - 30 Sep 2020
The present study had two aims: (1) To develop a culture system that imitates a normal physiological environment of primordial germ cells (PGCs). There are two types of PGCs in chicken: Circulating blood (cPGCs) and gonadal (gPGCs). The culture condition must support the [...] Read more.
The present study had two aims: (1) To develop a culture system that imitates a normal physiological environment of primordial germ cells (PGCs). There are two types of PGCs in chicken: Circulating blood (cPGCs) and gonadal (gPGCs). The culture condition must support the proliferation of both cPGCs and gPGCs, without affecting their migratory properties and must be deprived of xenobiotic factors, and (2) to propose an easy-to-train, nonlabeling optical technique for the routine identification of live PGCs. To address the first aim, early chicken embryo’s feeder cells were examined instead of using feeder cells from mammalian species. The KAv-1 medium at pH 8.0 with the addition of bFGF (basic fibroblast growth factor) was used instead of a conventional culture medium (pH approximately 7.2). Both cPGCs and gPGCs proliferated in vitro and retained their migratory ability after 2 weeks of culture. The cultivated cPGCs and gPGCs colonized the right and/or left gonads of the recipient male and female embryos. To address the second aim, we demonstrated a simple and rapid method to identify live PGCs as bright cells under darkfield illumination. The PGCs rich in lipid droplets in their cytoplasm highly contrasted with the co-cultured feeder layer and other cell populations in the culture. Full article
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