Advances in Equine Reproductive Immunology and Assisted Reproductive Techniques

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Equids".

Deadline for manuscript submissions: 10 July 2024 | Viewed by 718

Special Issue Editor

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Guest Editor
Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, 46115 Alfara del Patriarca, Spain
Interests: mare; embryo; ovulation; embryo–maternal communication; assisted reproductive techniques; embryo transfer; ovum pick up
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Special Issue Information

Dear Colleagues,

Embryo–maternal communication in mares plays an essential role in the overall equine fertility. Historically, research has focused on embryo loss after pregnancy diagnosis. Although this has a significant impact on the overall reproductive efficiency of a mare, more embryos are likely to be lost before this time. Understanding the relevance of endometrial and embryo factors involved in these losses is paramount to creating strategies to improve the efficiency of mare reproduction, both natural and that assisted by reproductive techniques.

Furthermore, more and more embryos are being produced in vitro, mainly by intra-cytoplasmic sperm injection (ICSI); therefore, more research is needed to study the effects of embryo survival and development after the transfer of in vitro cryopreserved embryos.

This Special Issue aims to increase the knowledge in the area of reproductive immunology, with special attention on the subfields of immunological aspects of embryo–maternal communication during early pregnancy in mares. A second objective is to study the embryo–maternal communication of in vivo-derived and in vitro-produced embryos during embryo transfer. Original research articles and reviews are welcome. Research areas may include (but are not limited to) the following: (1) embryo loss due to endometritis, (2) maternal recognition of pregnancy, (3) endometrial receptivity, and (4) assisted reproductive techniques.

I look forward to receiving your contributions.

Dr. Juan Cuervo-Arango
Guest Editor

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  • mare
  • embryo
  • maternal recognition of pregnancy

Published Papers (1 paper)

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11 pages, 1032 KiB  
Pregnancy and Luteal Responses to Embryo Reinsertion following Embryo Flushing in Donor Mares
by Rebeca Martínez-Boví, Jana T. H. Gaber, Laura Sala-Ayala, María Plaza-Dávila and Juan Cuervo-Arango
Animals 2024, 14(11), 1605; - 29 May 2024
Viewed by 482
The effect of embryo reinsertion immediately after embryo flushing was studied. In Experiment 1, eight mares were used during 32 cycles (8 cycles in each group). For the first two groups, inseminated mares were flushed 8 days after ovulation and prostaglandin F2α was [...] Read more.
The effect of embryo reinsertion immediately after embryo flushing was studied. In Experiment 1, eight mares were used during 32 cycles (8 cycles in each group). For the first two groups, inseminated mares were flushed 8 days after ovulation and prostaglandin F2α was not administered: in group EF-ET (embryo flushing and embryo transfer) the embryo was reinserted in the same donor mare, while in the EF group, no further procedure was performed. In the third group (ET), non-inseminated mares (recipients) received a Day 8 embryo. Progesterone concentration was measured before EF/ET and 72 h after in the three groups. In Experiment 2, twelve mares were used during 17 cycles in two groups, EF-ET (n = 11) and ET (n = 6), as in Experiment 1, except that every mare was flushed 24 h after embryo transfer to retrieve the embryo. Fewer pregnancies resulted after transfer in EF-ET cycles (0/8, 0%) than in the ET group (6/8, 75%). Progesterone concentration decreased significantly (p = 0.05) 72 h after EF-ET but not in EF or ET cycles (p > 0.1). Three mares from the EF-ET showed full luteolysis and signs of endometritis. In Experiment 2, more (5/6; p = 0.08) grade 1 embryos were recovered in the ET compared to the EF-ET group (3/7); 4 embryos were graded 3–4 (were broken or had signs of degeneration) in the EF-ET group but none in the ET group. In both groups, capsule fragments were obtained as indicative of the presence of a recently destroyed embryo in the EF-ET (n = 3) and ET (n = 1) groups. Positive bacterial cultures were obtained in 2/11 and 1/6 embryo flushes from the EF-ET and ET groups, respectively. Full article
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