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Animals
Special Issues
Sperm Quality Assessment in Domestic Animals
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Sperm Quality Assessment in Domestic Animals

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Animal Reproduction".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 1980

Special Issue Editors

Dr. Krzysztof Górski

E-Mail Website
Guest Editor
Institute of Animal Science and Fisheries, University of Siedlce, Bolesława Prusa 14, 08-110 Siedlce, Poland
Interests: sperm morphometry; sexual activity; sperm morphology; libido; spermiogram; ejaculate quality
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Stanisław Kondracki

E-Mail Website
Guest Editor
Institute of Animal Science and Fisheries, University of Siedlce, Bolesława Prusa 14, 08-110 Siedlce, Poland
Interests: sperm dimension; traits of ejaculate; artificial insemination; sexual activity; sperm morphology; spermiogram; sperm membrane integrity
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

High semen quality is a basic condition for male fertility. Progress in the field of utility traits of domestic animals does not support maintaining high male fertility. Therefore, research is needed on effective methods of semen assessment and factors determining sperm quality. The purpose of this Special Issue on “Sperm Quality Assessment in Domestic Animals” is to present the latest scientific achievements in the use of advanced techniques for assessments of sperm. We also invite original research articles that concern methods of semen preservation and storage. We look forward to receiving your contributions.

Original research articles and reviews are welcome.

Dr. Krzysztof Górski
Prof. Dr. Stanisław Kondracki
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Animals is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • sperm quality
  • sperm morphology
  • semen preservation
  • semen storage
  • sperm dimension

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Published Papers (2 papers)

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Research

16 pages, 1136 KB  
Article
Fourier Transform Infrared Spectroscopy to Measure Cholesterol in Goat Spermatozoa
by N. Cortés-Fernández-de-Arcipreste, A. J. Cardenas-Padilla, A. Alcantar-Rodriguez, A. Vázquez-Durán, A. Méndez-Albores and A. Medrano
Animals 2025, 15(21), 3107; https://doi.org/10.3390/ani15213107 (registering DOI) - 26 Oct 2025
Abstract
Sperm cryopreservation produces a series of physicochemical phenomena that negatively impact the function and structure of spermatozoa, including the mobilization of cholesterol from the plasma membrane. The use of attenuated total reflection–Fourier-transform infrared spectroscopy (ATR-FTIR) may be useful to measure the cholesterol efflux [...] Read more.
Sperm cryopreservation produces a series of physicochemical phenomena that negatively impact the function and structure of spermatozoa, including the mobilization of cholesterol from the plasma membrane. The use of attenuated total reflection–Fourier-transform infrared spectroscopy (ATR-FTIR) may be useful to measure the cholesterol efflux in goat spermatozoa. Therefore, the objective of this study was to standardize the use of ATR-FTIR to measure the efflux of cholesterol in goat spermatozoa. Standardization of the technique was carried out in three stages: (i) determination of the appropriate sperm concentration to detect cholesterol in the FTIR spectrum; (ii) determination of the minimum percentage of viable spermatozoa required to observe at least five spectral bands in common with pure cholesterol; (iii) assessment of cholesterol removal in frozen–thawed spermatozoa. Possible differences in the areas of the spectral bands were compared by one-way ANOVA. Nineteen spectra were obtained: pure cholesterol, sperm transport medium, five different sperm concentrations, and ten live/dead sperm proportions (heat and cold-killed). The lowest sperm concentration at which spectral bands were clearly identified was 13 × 106 sperm/mL. Regarding viability, the cut-off value was 50%: higher values produced spectral bands clearly detectable, whereas in smaller values, the band’s areas decreased sharply, making it difficult to quantify them. Five areas of the cholesterol bands decreased in thawed samples compared to fresh spermatozoa; an increase in the proportion of frozen–thawed sperm showing Merocyanine brilliant pattern, indicative of high fluidity, as well as an increase in the proportion of CTC AR pattern, indicative of acrosome reaction, support those results. In conclusion, ATR-FTIR is a useful technique for identifying the movement of cholesterol in goat buck spermatozoa. Full article
(This article belongs to the Special Issue Sperm Quality Assessment in Domestic Animals)
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13 pages, 3871 KB  
Article
Elevated Lipid Concentrations in Seminal Plasma Can Reduce Sperm Motility in Simmental Bulls
by Zhuo Yang, Fang Luo, Chenglei Song, Zhiyuan Ma, Yucheng Tian, Yu Fu, Hao Zheng and Jinzhong Tao
Animals 2025, 15(2), 276; https://doi.org/10.3390/ani15020276 - 20 Jan 2025
Cited by 1 | Viewed by 1434
Abstract
Sperm motility is a key factor influencing male fertility and is associated with metabolic and lipid profiles across species. The aim of this study was to investigate the relationship between sperm motility and the seminal plasma lipid profile in Simmental bulls, and to [...] Read more.
Sperm motility is a key factor influencing male fertility and is associated with metabolic and lipid profiles across species. The aim of this study was to investigate the relationship between sperm motility and the seminal plasma lipid profile in Simmental bulls, and to identify key lipids potentially influencing sperm motility. Semen samples were collected from 26 healthy Simmental bulls with an average age of 4.9 years. Sperm quality was evaluated using computer-assisted sperm analysis (CASA). Based on motility, the samples were divided into two groups: high sperm motility (HSM > 65%) and low sperm motility (LSM < 65%). Compared to the LSM group, the HSM group exhibited significantly higher sperm viability, motility, straight-line velocity, beat-cross frequency, and sperm acrosome integrity, while the sperm malformation rate was lower (p < 0.05). Lipid profiles were determined using LC-MS/MS, and 40 differential lipids were identified by multivariate statistical analysis. Among them, 39 lipids were upregulated in the LSM group compared to the HSM group. They were primarily triglycerides and carnitines, mainly involved in four metabolic pathways related to glycerophospholipid and linoleic acid metabolism. Notably, PC (16:0/20:4; 14:0/18:3), LPC (22:4/0:0; 22:6/0:0), and PE (14:0/18:1; 18:1/20:3) were diagnosed with great accuracy (AUC > 0.7), which means they may serve as potential biomarkers for sperm motility. Full article
(This article belongs to the Special Issue Sperm Quality Assessment in Domestic Animals)
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