Modern Reproductive Biotechnology Assists Farm Animal Conservation and Genetic Rescue
A special issue of Agriculture (ISSN 2077-0472). This special issue belongs to the section "Farm Animal Production".
Deadline for manuscript submissions: closed (15 May 2024) | Viewed by 13847
Special Issue Editors
Interests: reproductive biology and biotechnology; modern assisted reproductive technologies (ARTs); cryopreservation and culture engineering of gametes and embryos; in vitro fertilization (IVF) by either standard gamete co-incubation or intracytoplasmic sperm injection (ICSI); advanced approaches to cytobiochemically and molecularly evaluate the quality parameters of spermatozoa, oocytes and embryos; transgenic research; deciphering the transcriptomic and proteomic profiles in somatic cell lines and gametes; the ART-based programs of ex situ biodiversity conservation to protect the genetic resources of farm animals
Interests: reproductive biology and biotechnology in a wide variety of mammalian species (including livestock species); assisted reproductive technologies (ARTs)—experimental and applied embryology; intra- and interspecies cloning by somatic cell nuclear transfer (SCNT); transgenesis; parthenogenetic activation of oocytes; in vitro embryo production (IVP)—in vitro oocyte maturation (IVM), in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI); epigenetic mechanisms in somatic cell/stem cell and embryo culture engineering; unravelling the transcriptomic and proteomic profiles in somatic cell/stem cell lines and gametes; the ART-mediated programs of ex situ biodiversity conservation to protect the genetic resources of farm animals
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Special Issue Information
Dear Colleagues,
Within the framework of biodiversity protection programs, banking of the germplasm-carrier biological materials (male gametes and in vivo- or in vitro-produced embryos) by their ex situ preservation can be used as an ancillary strategy for not only maintaining and protecting the farm animal populations but also for the re-establishment and re-introduction into anthropogenic agricultural ecosystems of livestock breeds that disappear or significantly decrease in number. For all the above-mentioned reasons, the establishment and subsequent preservation of genetically stable or permanent primary cell cultures and somatic cell lines originating from different tissue bioptates provides an alternative or complementary research highlight in relation to gamete and embryo freezing or vitrification procedures.
The modern assisted reproductive technologies (ARTs) in farm animals are mainly based on in vitro embryo production (IVP) systems that include three inevitable steps, as has been consecutively indicated: the in vitro maturation (IVM) of oocytes, their in vitro fertilization (IVF) or reconstruction by somatic cell nuclear transfer (SCNT), and the in vitro culture (IVC) of fertilized or cloned embryos. Similar to other ARTs such as classic artificial insemination (AI), IVF frequently requires the use of cryopreserved or lyophilized spermatozoa, and can either be accomplished by standard gamete co-incubation, or can be assisted microsurgically by intracytoplasmic sperm injection (ICSI) into meiotically matured oocytes.
Innovative ART-mediated tools appear to be indispensable to genetically rescue and perpetuate the long-term ex situ conservation of biodiversity in indigenous breeds of various livestock species, including near-threatened, vulnerable, endangered, critically imperiled, vanishing and even extinct farm animal breeds.
Cumulatively, this Special Issue opens the possibility of publishing research articles, comprehensive reviews and short communications aimed at research highlights encompassing the efficient approaches that enable the protection of genetic resources derived from endangered farm animal species from extinction and to successfully retain the ex situ and/or in situ conservation of biodiversity in livestock species.
Dr. Monika Trzcińska
Prof. Dr. Marcin Samiec
Guest Editors
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Keywords
- assisted reproductive technologies (ARTs)
- ex situ and/or in situ protection of livestock species
- biodiversity conservation
- endangered or extinct livestock breeds
- genetic resources
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