- Article
Comparative Analysis and Ancestral Sequence Reconstruction of Bacterial Sortase Family Proteins Generates Functional Ancestral Mutants with Different Sequence Specificities
- Jordan D. Valgardson,
- Sarah A. Struyvenberg,
- Zachary R. Sailer,
- Isabel M. Piper,
- Justin E. Svendsen,
- D. Alex Johnson,
- Brandon A. Vogel,
- John M. Antos,
- Michael J. Harms and
- Jeanine F. Amacher
Gram-positive bacteria are some of the earliest known life forms, diverging from gram-negative bacteria 2 billion years ago. These organisms utilize sortase enzymes to attach proteins to their peptidoglycan cell wall, a structural feature that distin...

