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BioTech, Volume 10, Issue 3

2021 September - 10 articles

Cover Story: Salt-tolerant Artemisia vulgaris plants were developed through in vitro mutagenesis with the application of ethyl methane sulphonate (EMS) as the chemical mutagen. NaCl tolerance of the callus to maintain its growth under different concentrations ranges from 0 mM to 500 mM. Biochemical parameters such as chlorophyll, carotenoids, starch, amino acids, and phenol contents decreased under salt (NaCl) stress, whereas sugar and proline increased. Peroxidase (POD) and superoxide dismutase (SOD) activities peaked at 200 mM NaCl, whereas catalase (CAT) was high at 100 mM NaCl. Therefore, enhanced tolerance of the EMS-treated callus is attributed to the increased biochemical and antioxidant activity over the control and NaCl stress. View this paper.
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Articles (10)

  • Article
  • Open Access
10 Citations
6,713 Views
11 Pages

Increasing the High Throughput of a Luminescence-Based Serum Bactericidal Assay (L-SBA)

  • Maria Grazia Aruta,
  • Martina Carducci,
  • Francesca Micoli,
  • Francesca Necchi and
  • Omar Rossi

18 September 2021

Serum bactericidal assay (SBA) is the method to investigate in vitro complement-mediated bactericidal activity of sera raised upon vaccination. The assay is based on incubating the target bacteria and exogenous complement with sera at different dilut...

  • Article
  • Open Access
11 Citations
5,018 Views
12 Pages

21 August 2021

The present investigation aimed to obtain salt-tolerant Artemisia vulgaris L. to develop a constant form through in vitro mutagenesis with ethyl methane sulphonate (EMS) as the chemical mutagen. NaCl tolerance was evaluated by the ability of the call...

  • Review
  • Open Access
8 Citations
9,585 Views
15 Pages

9 August 2021

Estimating the fetal fraction of DNA in a pregnant mother’s blood is a risk-free, non-invasive way of predicting fetal aneuploidy. It is a rapidly developing field of study, offering researchers a plethora of different complementary methods. Such met...

  • Article
  • Open Access
7 Citations
9,093 Views
10 Pages

20 July 2021

This article aims to examine some of the ethical questions emerging from the use of already existing biotechnological tools and the issues which might occur by synthetic biology’s potential future possibilities. In the first part, the essence of synt...

  • Review
  • Open Access
56 Citations
29,091 Views
17 Pages

Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences

  • Kamand Tavakoli,
  • Alireza Pour-Aboughadareh,
  • Farzad Kianersi,
  • Peter Poczai,
  • Alireza Etminan and
  • Lia Shooshtari

Targeted nucleases are powerful genomic tools to precisely change the target genome of living cells, controlling functional genes with high exactness. The clustered regularly interspaced short palindromic repeats associated protein 9 (CRISPR-Cas9) ge...

  • Article
  • Open Access
21 Citations
6,780 Views
7 Pages

Background: The emergence of “multi-omics” and “multi-parametric” types of analysis based on a high number of biospecimens enforces the use of a great number of high-quality “Biological Materials and Associated Data” (BMaD). To meet the demands of bi...

  • Opinion
  • Open Access
7 Citations
7,494 Views
8 Pages

Treatment of Rheumatoid Arthritis with Gene Therapy Applications: Biosafety and Bioethical Considerations

  • Zinovia Tsitrouli,
  • Maria-Anna Akritidou,
  • Savvas Genitsaris and
  • Gijsbert van Willigen

23 June 2021

Rheumatoid Arthritis (RA) is an autoimmune and inflammatory disease that affects the synovium (lining that surrounds the joints), causing the immune system to attack its own healthy tissues. Treatment options, to the current day, have serious limitat...

  • Review
  • Open Access
26 Citations
12,429 Views
14 Pages

Biosafety of Genome Editing Applications in Plant Breeding: Considerations for a Focused Case-Specific Risk Assessment in the EU

  • Michael F. Eckerstorfer,
  • Marcin Grabowski,
  • Matteo Lener,
  • Margret Engelhard,
  • Samson Simon,
  • Marion Dolezel,
  • Andreas Heissenberger and
  • Christoph Lüthi

22 June 2021

An intensely debated question is whether or how a mandatory environmental risk assessment (ERA) should be conducted for plants obtained through novel genomic techniques, including genome editing (GE). Some countries have already exempted certain type...

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BioTech - ISSN 2673-6284