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BioTech, Volume 10, Issue 2

2021 June - 3 articles

Cover Story: Fusarium oxysporum is a serious threat to agricultural production. Due to its considerable variation of morphological and physiological makeup that results from an anamorphic species complex, it tends to elude detection. The compensatory characteristics of antimicrobial peptides (AMPs) have made them popular diagnostic biomarkers. This study used anti-Fusarium oxysporum antimicrobial peptides (AMPs) that have been experimentally validated to construct a profile against Fusarium oxysporum using HMMER. The AMPs had high binding energy scores with PR-1-like protein, Fpr1, with BOMK-10 having the strongest tendency to detect the fungus. The fundamental purpose of this diagnostic approach is to make it easier to find a standard reference for early detection of the fungus. AMPs have shown a lot of promise in terms of evading the drawbacks of the present diagnostic system. View this paper

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Article

41 Citations

8,899 Views

11 Pages

The 3Rs Principle in Animal Experimentation: A Legal Review of the State of the Art in Europe and the Case in Italy

Enrico Maestri

BioTech2021, 10(2), 9;https://doi.org/10.3390/biotech10020009

20 May 2021

The aim of this paper is to describe the essential points of Italian and European legislation governing the use of animals in biomedical experimentation. A close look will be taken at the principles of the 3Rs, which represent the mainstay of the leg...

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Article

8 Citations

4,568 Views

13 Pages

Harmonization of Biobank Education for Biobank Technicians: Identification of Learning Objectives

Mara Lena Hartung,
Ronny Baber,
Esther Herpel,
Cornelia Specht,
Daniel Peer Brucker,
Anne Schoneberg,
Theresa Winter and
Sara Yasemin Nussbeck

BioTech2021, 10(2), 7;https://doi.org/10.3390/biotech10020007

14 April 2021

The quality of biospecimens stored in a biobank depends tremendously on the technical personnel responsible for processing, storage, and release of biospecimens. Adequate training of these biobank employees would allow harmonization of correct sample...

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Article

5 Citations

5,004 Views

17 Pages

PR-1-Like Protein as a Potential Target for the Identification of Fusarium oxysporum: An In Silico Approach

Olalekan Olanrewaju Bakare,
Arun Gokul and
Marshall Keyster

BioTech2021, 10(2), 8;https://doi.org/10.3390/biotech10020008

14 April 2021

Fusarium oxysporum remains one of the leading causes of economic losses and poor crop yields; its detection is strained due to its presentation in various morphological and physiological forms. This research work sought to identify novel biomarkers f...

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