Appl. Nano, Volume 6, Issue 3 (September 2025) – 8 articles

Esophageal squamous cell carcinoma (ESCC) accounts for the majority of esophageal cancers worldwide, with a poor prognosis and increasing resistance to conventional treatments. Faced with these limitations, nanoparticles (NPs) are attracting growing interest as innovative therapeutic agents capable of improving specificity and efficacy and reducing systemic toxicity. This study critically examines the pharmacological effects, mechanisms of action, and toxicity profiles of different metallic or organic nanoparticles tested on ESCC cell lines. Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) 2020 guidelines were followed by a meticulous literature search of Google Scholar, Web of Science, PubMed/Medline, and Scopus databases to achieve this goal. The results show that the anti-tumor properties vary according to the type of nanoparticle (copper(II) oxide (CuO), silver (Ag), gold (Au), nickel(II) oxide (NiO), nano-curcumin, etc.), the synthesis method (chemical vs. green), and the biological activity assessment method (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Bromodeoxyuridine (BrdU), Cell Counting Kit-8 (CCK8) assays, etc.). NPs derived from green synthesis, such as those based on Moringa oleifera, Photinia glabra, or pomegranate bark, exhibit moderate cytotoxic activity (50% inhibitory concentration (IC₅₀) between 92 and 500 µg/mL) but show good tolerance on normal cells. In contrast, chemically synthesized NPs, such as Cu(II) complexes with 1,3,5-benzenetricarboxylic acid (H₃btc) or 1,2,4-triazole (Htrz), show lower IC₅₀ (34–86 µM), indicating more marked cytotoxicity towards cancer cells, although data on their toxicity are sometimes lacking. In addition, multifunctional nanoparticles, such as gold-based nano-conjugates targeting Cluster of Differentiation 271 (CD271) or systems combined with doxorubicin, show remarkable activity with IC₅₀ below 3 µM and enhanced tumor selectivity, positioning them among the most promising candidates for future clinical application against ESCC. The most frequently observed mechanisms of action include induction of apoptosis (↑caspases, ↑p53, ↓Bcl-2), oxidative stress, and inhibition of proliferation. In conclusion, this work identifies several promising nanoparticles (silver nanoparticles derived from Photinia glabra (PG), gold-based nano-immunoconjugates targeting CD271, and silver–doxorubicin complexes) for future pharmaceutical exploitation against ESCC. However, major limitations remain, such as the lack of methodological standardization, insufficient in vivo and clinical studies, and poor industrial transposability. Future prospects include the development of multifunctional nanocomposites, the integration of biomarkers for personalized targeting, and long-term toxicological assessment. Full article

Localized therapeutic action and targeted drug release offer compelling advantages over traditional systemic drug administration. This is particularly important for nitric oxide (NO), whose biological effects vary greatly depending on concentration and cellular environment. Light-sensitive NO donors are promising for achieving precise, on-demand NO release. However, their efficiency and photostability are limited by competing photophysical processes and the generation of reactive oxygen species (ROS). In this study, we investigate hybrid systems composed of photosensitive nitric oxide (NO) donors and silver island films (SIFs). The influence of localized surface plasmon on non-radiative relaxation pathways and ROS generation is the main focus of the paper. Upon excitation at 500 nm, we observed several-fold increase in NO release, attributed to resonant interactions between the plasmonic field and the dye molecules. By tuning the thickness of a SiO₂ buffer layer, we identified key parameters affecting process efficiency: the spectral overlap between the plasmon resonance and the sensitizer’s absorption band, and the distance between the nanoparticle and the molecule. Additionally, singlet oxygen generation increase was observed. These findings demonstrate the potential of plasmonic enhancement to controllably boost photochemical activity in organic systems, paving the way for advanced applications in phototherapy and biomedical diagnostics. Full article

Hydrogen is emerging as a key energy vector for the transition toward renewable and sustainable energy sources. However, its safe and efficient storage remains a significant technical challenge in terms of cost, safety, and performance. In this study, we aimed to address the kinetic limitations of Mg by synthesizing catalyzed Mg@Ni systems using commercially available micrometric magnesium particles (~26 µm), which were decorated via electroless nickel plating under both aqueous and anhydrous conditions. Morphological and compositional characterization was carried out using SEM, EDS, and XRD. The resulting materials were evaluated through Temperature-Programmed Desorption (TPD), DSC, and isothermal hydrogen absorption/desorption kinetics. Reversibility over multiple absorption–desorption cycles was also investigated. The synthesized Mg@NiB system shows a reduction of 37 °C in the hydrogen release activation temperature at atmospheric pressure and a decrease of 167.3 °C under high vacuum conditions (4.5 × 10⁻⁷ MPa), in addition to a reversible hydrogen absorption/desorption capacity of 3.5 ± 0.09 wt.%. Additionally, the apparent activation energy for hydrogen desorption was lower (161.7 ± 21.7 kJ/mol) than that of hydrogenated commercial pure magnesium and was comparable to that of milling MgH₂ systems. This research is expected to contribute to the development of efficient and low-cost processing routes for large-scale Mg catalysis. Full article

Polymer matrix composites (PMCs) are crucial for their applications in aerospace, electronics, defense, and structural materials. PMCs reinforced with nanofillers offer substantial potential for enhanced thermal and mechanical performance. Although there have been significant developments in nanofiller-based high-performance composites involving graphene, carbon nanotubes, and metal oxides, the smallest of all the fillers, the graphene quantum dot (GQD), has not been explored thoroughly. The objective of this study is to investigate the effects of GQDs on the thermal properties of epoxy nanocomposites using all-atom molecular dynamics (MD) simulations. Specifically, the influence of GQDs on the glass transition temperature (T_g) and coefficient of linear thermal expansion (CTE) of the bisphenol F epoxy is evaluated. Further, the effects of surface functionalization and edge functionalization of GQDs are analyzed. Results demonstrate that the inclusion of functionalized GQDs leads to a 16% improvement in T_g, attributed to enhanced interfacial interactions and restricted molecular mobility in the epoxy network. MD simulations reveal that functional groups on GQDs form strong physical and chemical interactions with the polymer matrix, effectively altering its dynamics at the T_g. These results provide key molecular-level insights into the design of the next generation of thermally stable epoxy nanocomposites for high-performance applications in aerospace and defense. Full article

Amino acids are not just monomers of proteins, but they can also carry biological functions. L-cysteine (Cys), L-proline (Pro), L-asparagine (Asn), and L-glutamic acid (Glu) were used to evaluate how different amino acid chemistries alter the morphology and size of the silver nanoparticles (AgNPs) synthesized in the presence of two carbohydrate ligands, which were lactose methoxyaniline (LMA) and galactose 5-aminosalicylic acid (G5AS). UV–vis, infrared (IR), High-Resolution Transmission Electron Microscopy (HR-TEM) and X-ray diffraction (XRD) characterizations revealed that the effect of amino acids on the characteristics of the AgNPs showed dependence on the carbohydrate ligand chemistry. In the case of LMA, AgNPs shifted from aggregates to anisotropic nanoparticles, larger aggregates, and a mixture of anisotropic and 1D nanoparticles in the presence of Cys, Glu, Asn and Pro amino acids, respectively. In contrast to this, the introduction of Cys and Asn caused the formation of cluster-like AgNPs and larger rounded nanoparticles, while G5AS-synthesized AgNPs were multigonal 0D particles. Moreover, Glu and Pro contributed the resistance of silver oxide formation on the particles. Antibacterial characterization showed that LMA_Glu_AgNPs were the most effective ones, while LMA_Cys_AgNPs and G5AS_Cys_AgNPs, which were the smallest AgNPs, did not show any significant antibacterial activity. Full article

Super-resolution microscopy (SRM) has revolutionized our understanding of subcellular structures, including cell organelles and viruses. For human immunodeficiency virus (HIV), SRM has significantly advanced knowledge of viral structural biology and assembly dynamics. This review analyzes how SRM techniques (particularly PALM, STORM, STED, and SIM) have been applied over the past decade to study HIV structural components and assembly. By categorizing and comparing studies based on SRM methods, HIV components, and labeling strategies, we assess the strengths and limitations of each approach. Our analysis shows that PALM is most commonly used for live-cell imaging of HIV Gag, while STED is primarily used to study the viral envelope (Env). STORM and SIM have been applied to visualize various components, including Env, capsid, and matrix. Antibody labeling is prevalent in PALM and STORM studies, targeting Env and capsid, whereas fluorescent protein labeling is mainly associated with PALM and focused on Gag. A recent emphasis on Gag and Env points to deeper investigation into HIV assembly and viral membrane dynamics. Insights from SRM studies of HIV not only enhance virological understanding but also inform future research in therapeutic strategies and delivery systems, including extracellular vesicles. Full article

Carbon nanotubes (CNTs) are cylindrical nanostructures formed by rolling a graphene sheet—a hexagonal lattice of carbon atoms—into a tube. Based on the rolling direction, CNTs are categorized as armchair, zigzag, or chiral. The chiral vector, derived from the graphene lattice, defines the CNT’s structure, with chiral CNTs denoted by indices (n, m), where m > 0 and m ≠ n. The mechanical properties and structural stability of CNTs are highly sensitive to defects and impurities within their atomic framework. Among these, point defects such as single-atom vacancies are the most prevalent and can significantly degrade mechanical performance. These defects alter stress distribution, reduce stiffness, and impair strength, thereby limiting the functional reliability of CNTs in advanced applications such as nanocomposites, sensors, and electronic devices. This study examines the influence of vacancy defects on CNT mechanical behavior through a multiscale modeling framework. Molecular dynamics (MD) simulations are conducted using LAMMPS, with structural visualization via Visual Molecular Dynamics (VMD). Concurrently, a finite element (FE) model is developed in ANSYS, where the CNT is idealized as a space frame of elastic beam elements representing carbon–carbon bonds. The integration of atomistic and continuum approaches offers a comprehensive understanding of defect-induced mechanical degradation. The MD and FEM results are in strong agreement with findings in existing literature, validating the adopted methodology. These findings contribute valuable insights into the design and optimization of CNT-based materials for high-performance engineering applications. Full article

This study investigates the development and sensing profile of synthetic melanin nanoparticle-coated electrodes for the electrochemical detection of heavy metals, including lead (Pb), cadmium (Cd), cobalt (Co), zinc (Zn), nickel (Ni), and iron (Fe). Synthetic melanin films were prepared in situ by the deacetylation of diacetoxy indole (DAI) to dihydroxy indole (DHI), followed by the deposition of DHI monomers onto indium tin oxide (ITO) and glassy carbon electrodes (GCE) using cyclic voltammetry (CV), forming a thin layer of synthetic melanin film. The deposition process was characterized by electrochemical quartz crystal microbalance (EQCM) in combination with linear sweep voltammetry (LSV) and amperometry to determine the mass and thickness of the deposited film. Surface morphology and elemental composition were examined using scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). In contrast, Fourier-transform infrared (FTIR) and UV–Vis spectroscopy confirmed the melanin’s chemical structure and its polyphenolic functional groups. Differential pulse voltammetry (DPV) and amperometry were employed to evaluate the melanin films’ electrochemical activity and sensitivity for detecting heavy metal ions. Reproducibility and repeatability were rigorously assessed, showing consistent electrochemical performance across multiple electrodes and trials. A comparative analysis of ITO, GCE, and graphite electrodes was conducted to identify the most suitable substrate for melanin film preparation, focusing on stability, electrochemical response, and metal ion sensing efficiency. Finally, the applicability of melanin-coated electrodes was tested on in-house heavy metal water samples, exploring their potential for practical environmental monitoring of toxic heavy metals. The findings highlight synthetic melanin-coated electrodes as a promising platform for sensitive and reliable detection of iron with a sensitivity of 106 nA/ppm and a limit of quantification as low as 1 ppm. Full article