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Methods and Protocols, Volume 5, Issue 2

2022 April - 15 articles

Cover Story: The reprogramming of somatic cells to generate induced pluripotent stem cells (iPSCs) provides important applications for fields such as regenerative medicine and disease modeling. However, the low efficiency and slow kinetics of the reprogramming process have greatly hampered improvements with this technology. To address this issue, we generated a practical workflow with multiple steps, including plasmids transfection, viral transduction, cells preparation, and reprogrammed cells identification, allowing the rapid and efficient induction of iPSCs from mouse embryonic fibroblasts (MEFs) using a chemically defined medium iCD1. This workflow can be easily implemented in a standard cell culture laboratory, thus helping researchers to explore the fields of cell fate research. View this paper
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Articles (15)

  • Article
  • Open Access
6 Citations
5,297 Views
16 Pages

Lipedema is a multifaceted chronic fat disorder characterized by the bilateral and disproportionate accumulation of fat predominantly in the lower body regions of females. Research strongly supports that estrogen factors likely contribute to the path...

  • Protocol
  • Open Access
4 Citations
4,327 Views
16 Pages

Implementing a Rural Natural Experiment: A Protocol for Evaluating the Impacts of Food Coops on Food Consumption, Resident’s Health and Community Vitality

  • Éric Robitaille,
  • Marie-Claude Paquette,
  • Gabrielle Durette,
  • Amélie Bergeron,
  • Marianne Dubé,
  • Mélanie Doyon,
  • Geneviève Mercille,
  • Marc Lemire and
  • Ernest Lo

Background: Local food environments are recognized by experts as a determinant of healthy eating. Food cooperatives (coop) can promote the accessibility to healthier foods and thus improve the health of the population, particularly in remote rural co...

  • Protocol
  • Open Access
1 Citations
3,525 Views
8 Pages

Medication administration is recognized as a risk-prone activity where errors and near misses have multiple opportunities to occur along the route from manufacturing, through transportation, storage, prescription, dispensing, point-of-care administra...

  • Article
  • Open Access
7 Citations
4,070 Views
8 Pages

Assessment of Sodium Diamine Fluoride (SDF) with Light Curing Technique: A Pilot Study of Antimicrobial Effects

  • Jens Wilson,
  • Sarah Swanbeck,
  • Gavin Banning,
  • Tatiana Alhwayek,
  • Victoria Sullivan,
  • Katherine M. Howard and
  • Karl Kingsley

Silver diamine fluoride (SDF) has been useful in clinical dentistry for the purpose of caries arrest and prevention. Although methods for the application of SDF are well-known among dental professionals, such as microbrush applications, few studies h...

  • Article
  • Open Access
10 Citations
8,427 Views
11 Pages

How to Clean and Safely Remove HF from Acid Digestion Solutions for Ultra-Trace Analysis: A Microwave-Assisted Vessel-Inside-Vessel Protocol

  • Marco Pinna,
  • Arianna Signorelli,
  • Gilberto Binda,
  • Carlo Dossi,
  • Laura Rampazzi,
  • Davide Spanu and
  • Sandro Recchia

The complete dissolution of silicate-containing materials, often necessary for elemental determination, is generally performed by microwave-assisted digestion involving the forced use of hydrofluoric acid (HF). Although highly efficient in dissolving...

  • Protocol
  • Open Access
15 Citations
10,638 Views
10 Pages

Plankton, at the bottom of the food web, play a central role in the entry of mercury into the aquatic biota. To investigate their role in mercury uptake, reliable analytical procedures for Hg analysis are highly sought. Wet digestion procedures for d...

  • Protocol
  • Open Access
5,053 Views
11 Pages

Fast and Efficient Mouse Pluripotency Reprogramming Using a Chemically-Defined Medium

  • Junju Huang,
  • Xuejie Yang,
  • Jie Wang,
  • Haoyu Wu,
  • Duanqing Pei and
  • Jiekai Chen

The reprogramming of somatic cells to obtain induced pluripotent stem cells (iPSCs) is an important biological and medical breakthrough, providing important applications for fields such as regenerative medicine and disease modeling. However, this pro...

  • Article
  • Open Access
3 Citations
5,210 Views
20 Pages

Interactive Analysis, Exploration, and Visualization of RNA-Seq Data with SeqCVIBE

  • Efthimios Bothos,
  • Pantelis Hatzis and
  • Panagiotis Moulos

The rise of modern gene expression profiling techniques, such as RNA-Seq, has generated a wealth of high-quality datasets spanning all fields of current biological research. The large data sets and the continually expanding applications for which the...

  • Protocol
  • Open Access
7 Citations
5,673 Views
23 Pages

Genomic Fishing and Data Processing for Molecular Evolution Research

  • Héctor Lorente-Martínez,
  • Ainhoa Agorreta and
  • Diego San Mauro

Molecular evolution analyses, such as detection of adaptive/purifying selection or ancestral protein reconstruction, typically require three inputs for a target gene (or gene family) in a particular group of organisms: sequence alignment, model of ev...

  • Protocol
  • Open Access
4 Citations
5,490 Views
11 Pages

Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples

  • Francesco Saverio Tarantini,
  • Siyu Wu,
  • Harry Jenkins,
  • Ana Tellechea Lopez,
  • Hannah Tomlin,
  • Ralph Hyde,
  • Katarzyna Lis-Slimak,
  • Jamie Louise Thompson,
  • Sara Pijuan-Galitó and
  • Andrew V. Benest
  • + 10 authors

Since mid-2020 there have been complexities and difficulties in the standardisation and administration of nasopharyngeal swabs. Coupled with the variable and/or poor accuracy of lateral flow devices, this has led to increased societal ‘testing...

  • Article
  • Open Access
4 Citations
3,565 Views
13 Pages

Validation of In Vivo Nodal Assessment of Solid Malignancies with USPIO-Enhanced MRI: A Workflow Protocol

  • Daphne A. J. J. Driessen,
  • Didi J. J. M. de Gouw,
  • Rutger C. H. Stijns,
  • Geke Litjens,
  • Bas Israël,
  • Bart W. J. Philips,
  • John J. Hermans,
  • Tim Dijkema,
  • Bastiaan R. Klarenbeek and
  • Tom W. J. Scheenen
  • + 6 authors

Background: In various cancer types, the first step towards extended metastatic disease is the presence of lymph node metastases. Imaging methods with sufficient diagnostic accuracy are required to personalize treatment. Lymph node metastases can be...

  • Protocol
  • Open Access
4 Citations
3,673 Views
14 Pages

In the context of increased rates of frailty and chronic disease among older people, there is a need to develop age-friendly, integrated primary care models that place the older person at the centre of their care. However, there is little evidence ab...

  • Protocol
  • Open Access
5 Citations
6,232 Views
11 Pages

Risk factors that predispose individuals towards major non-communicable diseases (NCDs) and periodontal disease (PD) often co-occur in the same individual. The common risk factor approach (CRFA) for controlling the risk factors associated with NCDs a...

  • Article
  • Open Access
8 Citations
4,465 Views
11 Pages

Retinoblastoma (RB) is a primary intraocular malignancy in childhood. Relapses may develop and cause secondary cancers during later development. This study was set up to identify optimal cell culture conditions for RB cell growth in vitro and to opti...

  • Protocol
  • Open Access
7 Citations
11,277 Views
6 Pages

Optimization of Peripheral Blood Mononuclear Cell Extraction from Small Volume of Blood Samples: Potential Implications for Children-Related Diseases

  • Deanira Patrone,
  • Nicola Alessio,
  • Nicola Antonucci,
  • Anna Lisa Brigida,
  • Gianfranco Peluso,
  • Umberto Galderisi and
  • Dario Siniscalco

24 February 2022

Managing medical procedures for children with problematic disorders is a challenging approach, especially in the case of blood withdrawal for autism spectrum disorder-affected children. Peripheral blood mononuclear cells (PBMC) represent an important...

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Methods Protoc. - ISSN 2409-9279