Next Article in Journal
End-User Software for Efficient Sensor Placement in Jacketed Wine Tanks
Next Article in Special Issue
Micro- and Nanoscale Approaches in Antifungal Drug Discovery
Previous Article in Journal
Acetogen Communities in the Gut of Herbivores and Their Potential Role in Syngas Fermentation
Previous Article in Special Issue
Citric Acid Production by Yarrowia lipolytica Yeast on Different Renewable Raw Materials
Article Menu
Issue 2 (June) cover image

Export Article

Open AccessArticle
Fermentation 2018, 4(2), 41;

Fluorinated Phenylalanine Precursor Resistance in Yeast

Biomolecular Sciences Institute, Florida International University, Miami 33199, FL, USA
Author to whom correspondence should be addressed.
Received: 27 April 2018 / Revised: 2 June 2018 / Accepted: 4 June 2018 / Published: 9 June 2018
(This article belongs to the Special Issue Yeast Biotechnology 2.0)
Full-Text   |   PDF [4873 KB, uploaded 9 June 2018]   |  


Development of a counter-selection method for phenylalanine auxotrophy could be a useful tool in the repertoire of yeast genetics. Fluorinated and sulfurated precursors of phenylalanine were tested for toxicity in Saccharomyces cerevisiae. One such precursor, 4-fluorophenylpyruvate (FPP), was found to be toxic to several strains from the Saccharomyces and Candida genera. Toxicity was partially dependent on ARO8 and ARO9, and correlated with a strain’s ability to convert FPP into 4-fluorophenylalanine (FPA). Thus, strains with deletions in ARO8 and ARO9, having a mild phenylalanine auxotrophy, could be separated from a culture of wild-type strains using FPP. Tetrad analysis suggests FPP resistance in one strain is due to two genes. Strains resistant to FPA have previously been shown to exhibit increased phenylethanol production. However, FPP resistant isolates did not follow this trend. These results suggest that FPP could effectively be used for counter-selection but not for enhanced phenylethanol production. View Full-Text
Keywords: fluorinated compounds; counter selection; phenylalanine; phenylethanol; yeast fluorinated compounds; counter selection; phenylalanine; phenylethanol; yeast

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Murdoch, I.S.; Powers, S.L.; Welch, A.Z. Fluorinated Phenylalanine Precursor Resistance in Yeast. Fermentation 2018, 4, 41.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Statistics



[Return to top]
Fermentation EISSN 2311-5637 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top