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Effect of Marine Basidiomycetes Fulvifomes sp.-Derived Ergosterol Peroxide on Cytotoxicity and Apoptosis Induction in MCF-7 Cell Line

1
Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai 608 502, India
2
Molecular Biology Group, National Institute of Cancer Prevention and Research, Noida 201 301, India
3
Experimental Cancer Therapeutics and Chemical Biology Laboratory, UM-DAE Centre for Excellence in Basic Sciences, Kalina, Mumbai 400098, India
*
Author to whom correspondence should be addressed.
J. Fungi 2019, 5(1), 16; https://doi.org/10.3390/jof5010016
Received: 15 December 2018 / Revised: 8 February 2019 / Accepted: 11 February 2019 / Published: 13 February 2019
(This article belongs to the Special Issue Fungal Polyketides and Other Secondary Metabolites)
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Abstract

The aim of the present study is to extract the bioactive compounds which can induce the apoptosis in breast cancer cell line MCF-7 by marine basidiomycetes. Internal Transcribed Spacer (ITS) sequences based molecular taxonomic study confirmed that collected the marine basidiomycetes belongs to Fulvifomes sp. Further, the isolated compounds from the Fulvifomes sp. confirmed as ergosterol peroxide (EP) by spectroscopic studies. The compound inhibited 50% of the cell growth (IC50) at the concentration of 40 µg/mL and induced 90% cell death (IC 90) at the concentration of 80 µg/mL. The ergosterol peroxide generated Reactive Oxygen Species (ROS) and induced apoptotic cell death in MCF-7. Ethidium bromide/Acridine Orange (Et/Br) staining showed the increased number of early and late apoptosis in treated MCF-7 cells. The compounds treated cells indicated the significant loss of mitochondrial membrane potential (Δψm) with p < 0.05. The induction of apoptosis by marine basidiomycetes derived ergosterol peroxide was confirmed by chromatin condensation in MCF7 cells using Hoechst staining 33342. View Full-Text
Keywords: Marine Basidiomycetes; ergosterol peroxide; apoptosis induction; breast cancer Marine Basidiomycetes; ergosterol peroxide; apoptosis induction; breast cancer
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Govindharaj, M.; Arumugam, S.; Nirmala, G.; Bharadwaj, M.; Murugiyan, K. Effect of Marine Basidiomycetes Fulvifomes sp.-Derived Ergosterol Peroxide on Cytotoxicity and Apoptosis Induction in MCF-7 Cell Line. J. Fungi 2019, 5, 16.

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