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Vet. Sci. 2018, 5(3), 78; https://doi.org/10.3390/vetsci5030078

In Vitro Anthelmintic Activity of Four Plant-Derived Compounds against Sheep Gastrointestinal Nematodes

1
Dipartimento di Scienze Veterinarie, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy
2
Dipartimento di Farmacia, University of Pisa, Via Bonanno Pisano 6, 56126 Pisa, Italy
*
Author to whom correspondence should be addressed.
Received: 10 July 2018 / Revised: 26 August 2018 / Accepted: 6 September 2018 / Published: 10 September 2018
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Abstract

By using the egg hatch test (EHT), the larval development test (LDT) and the larval mortality/paralysis test (LMT), the in vitro anthelmintic activity on sheep gastrointestinal strongyles (GIS) of four plant-derived pure compounds, mangiferin (at 0.25%, 0.125% and 0.0625%), rutin (at 1%, 0.75%, 0.5%), quercetin (at 1%), and β-sitosterol (at 1%, 0.75%, 0.5%), was investigated. For comparison, untreated and treated (0.1% thiabendazole, 0.1% TBZ) controls were used. Six repetitions were made throughout the experiment. Data were statistically elaborated using the χ2 test. The concentration able to inhibit the development of the 50% of L1s to L3s and causing the mortality of the 50% of L3s (EC50) was also calculated. L3s recovered from untreated Petri dishes were identified at the genus level. In EHT, all tested compounds at all concentrations significantly (p < 0.01) inhibited the hatch of the eggs when compared to the untreated controls, but none of them was as effective as 0.1% TBZ. In LDT, rutin (at 1%, 0.75% and 0.5%), mangiferin (at 0.25% and 0.125%), β-sitosterol (at 1%) and 0.1% TBZ completely prevented the larval development from L1 to L3 in respect to the untreated controls (p < 0.01). In LMT, all tested compounds significantly (p < 0.01) increased the death of L3s compared to the untreated controls, except for β-sitosterol at 0.5%. However, only rutin at all concentrations and 0.25% and 0.125% mangiferin were as effective as 0.1% TBZ. Haemonchus, Trichostrongylus, Chabertia and Teladorsagia/Ostertagia GIS genera, were identified. View Full-Text
Keywords: mangiferin; rutin; quercetin; β-sitosterol; anthelmintic activity; in vitro; gastrointestinal strongyles; sheep mangiferin; rutin; quercetin; β-sitosterol; anthelmintic activity; in vitro; gastrointestinal strongyles; sheep
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Giovanelli, F.; Mattellini, M.; Fichi, G.; Flamini, G.; Perrucci, S. In Vitro Anthelmintic Activity of Four Plant-Derived Compounds against Sheep Gastrointestinal Nematodes. Vet. Sci. 2018, 5, 78.

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