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Biocatalyst Potential of Cellulose-Degrading Microorganisms Isolated from Orange Juice Processing Waste

Laboratory of Wastewater Management and Treatment Technologies, Department of Environmental Engineering, Democritus University of Thrace, Vas. Sofias 12, 67132 Xanthi, Greece
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Beverages 2019, 5(1), 21; https://doi.org/10.3390/beverages5010021
Received: 29 December 2018 / Revised: 3 February 2019 / Accepted: 11 February 2019 / Published: 2 March 2019
(This article belongs to the Special Issue Novel Beverages and Novel Technologies for Their Production)
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Abstract

Cellulases can be applied as macerating and peeling enzymes in the orange juice processing industry. In this work, indigenous cellulose-degrading microorganisms were isolated from orange juice processing waste through successive enrichment procedures using carboxymethyl cellulose (CMC) as the sole carbon source. A total of 24 microbial isolates were screened for their ability to grow in CMC liquid medium, resulting in the selection of seven isolates. The latter were further assessed by determining their endo-1,4-β-d-glucanase, exo-1,4-β-d-glucanase, and β-1,4-d-glucosidase activities, of which their respective activities were as high as 3.89, 10.67, and 10.69 U/mg protein. All cellulose-degraders selected belonged to the genus Paenibacillus, although to distinct operational taxonomic units related to P. xylanexedens, P. tundrae, and P. pabuli (operational taxonomic unit—OTU#1) and to P. wynnii, P. odorifer, and P. donghaensis (OTU#2) spectrum. Regarding the cellulase activities of the orange juice processing waste, endo-1,4-β-d-glucanase activity (4.00 ± 0.11 U/g) was exerted only extracellularly, whereas exo-1,4-β-d-glucanase (2.60 ± 0.19 U/g) and β-1,4-d-glucosidase (5.69 ± 0.23 U/g) activities were exhibited both extracellularly and intracellularly. In conclusion, orange juice processing waste can be considered as a valuable source for the isolation of cellulose-degrading microbiota with potential uses in beverage industry, solid state fermentation and energy production. View Full-Text
Keywords: cellulolytic bacteria; cellulase; glucanase; glucosidase; beverage industry; solid state fermentation; Paenibacillus cellulolytic bacteria; cellulase; glucanase; glucosidase; beverage industry; solid state fermentation; Paenibacillus
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Zerva, I.; Remmas, N.; Ntougias, S. Biocatalyst Potential of Cellulose-Degrading Microorganisms Isolated from Orange Juice Processing Waste. Beverages 2019, 5, 21.

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