Enhanced Effects of Iron on Mycelial Growth, Metabolism and In Vitro Antioxidant Activity of Polysaccharides from Lentinula edodes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Strain and Culture Conditions
2.2. Assessment of Optimal Fe2+ Concentration and Induction Time
2.3. Biomass and Polysaccharides Determination
2.4. Dynamic Changes in Biomass and Polysaccharides Production
2.5. Effects of Fe 2+ on Polysaccharide Synthesis
2.5.1. Enzyme Activity Assays
2.5.2. Transcriptional Expression Analysis
2.6. In Vitro Antioxidant Activities of Polysaccharides
2.7. Statistical Analysis
3. Results
3.1. Assessment of Optimal Fe2+ Concentration and Induction Time
3.2. Dynamic Changes in the Mycelial Biomass and Polysaccharides Production
3.3. Effects of Fe2+ on Polysaccharide Synthesis
3.3.1. Enzyme Activity Assays
3.3.2. Transcriptional Expression Analysis
3.4. Effect of Fe2+ on the In Vitro Antioxidant Activity of Polysaccharides
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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PGI | PGM | UGP | |
---|---|---|---|
IPS | −0.262 | 0.867 ** | 0.869 ** |
EPS | −0.385 | 0.177 | 0.379 |
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Xiang, Q.; Zhang, H.; Chen, X.; Hou, S.; Gu, Y.; Yu, X.; Zhao, K.; Zhang, X.; Ma, M.; Chen, Q.; et al. Enhanced Effects of Iron on Mycelial Growth, Metabolism and In Vitro Antioxidant Activity of Polysaccharides from Lentinula edodes. Bioengineering 2022, 9, 581. https://doi.org/10.3390/bioengineering9100581
Xiang Q, Zhang H, Chen X, Hou S, Gu Y, Yu X, Zhao K, Zhang X, Ma M, Chen Q, et al. Enhanced Effects of Iron on Mycelial Growth, Metabolism and In Vitro Antioxidant Activity of Polysaccharides from Lentinula edodes. Bioengineering. 2022; 9(10):581. https://doi.org/10.3390/bioengineering9100581
Chicago/Turabian StyleXiang, Quanju, Huijuan Zhang, Xiaoqian Chen, Shiyao Hou, Yunfu Gu, Xiumei Yu, Ke Zhao, Xiaoping Zhang, Menggen Ma, Qiang Chen, and et al. 2022. "Enhanced Effects of Iron on Mycelial Growth, Metabolism and In Vitro Antioxidant Activity of Polysaccharides from Lentinula edodes" Bioengineering 9, no. 10: 581. https://doi.org/10.3390/bioengineering9100581