The Impact of Germination on Sorghum Nutraceutical Properties
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. Plant Material and Germination Process
2.3. Plant Material Extraction
2.4. Total Phenolic Content Determination
2.5. Determination of Antioxidant Activity
2.6. Peptide Extraction
2.7. Angiotensin-Converting Enzyme (ACE) Inhibitory Activity
2.8. Statistical Analysis
3. Results
3.1. Total Phenolic Content and In Vitro Antioxidant Activities
3.2. Cellular Antioxidant Activity in Red Blood Cells (CAA-RBC)
3.3. ACE Inhibitory Activity of Sorghum Peptides
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
AAPH | 2,2-azobis(2-amidinopropane) dihydrochloride |
ACE | angiotensin-converting enzyme |
CAA-RBC | cellular antioxidant activity in red blood cells |
DCFH-DA | 2′,7′-dichlorofluorescein diacetate |
DPPH | 2,2-diphenyl-1-picrylhydrazyl |
FRAP | ferric reducing antioxidant power |
GS | germinated sorghum |
NGS | non-germinated sorghum |
TPC | total phenolic content |
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Total Phenolic Content (mg GAE/g DW) | DPPH EC50 (mg/mL) | FRAP (µM Fe2+) | |
---|---|---|---|
Germinated | 6.38 ± 0.37 | 0.51 ± 0.01 | 2666 ± 23 |
Non-germinated | 6.62 ± 0.28 | 0.29 ± 0.05 *** | 3636.2 ± 124.68 **** |
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Arouna, N.; Gabriele, M.; Pucci, L. The Impact of Germination on Sorghum Nutraceutical Properties. Foods 2020, 9, 1218. https://doi.org/10.3390/foods9091218
Arouna N, Gabriele M, Pucci L. The Impact of Germination on Sorghum Nutraceutical Properties. Foods. 2020; 9(9):1218. https://doi.org/10.3390/foods9091218
Chicago/Turabian StyleArouna, Nafiou, Morena Gabriele, and Laura Pucci. 2020. "The Impact of Germination on Sorghum Nutraceutical Properties" Foods 9, no. 9: 1218. https://doi.org/10.3390/foods9091218