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Open AccessArticle

Impact of Simulated Gastrointestinal Digestion on the Biological Activity of an Alcalase Hydrolysate of Orange Seed (Siavaraze, Citrus sinensis) by-Products

1
Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Avenue Agustín Escardino 7, Paterna, 46980 Valencia, Spain
2
Department of Food Science & Technology, Gorgan University of Agricultural Sciences & Natural Resources, Gorgan 4918943464, Iran
3
The Health of Plant and Livestock Products Research Center, Department of Pharmacology, Mazandaran University of Medical Sciences, Sari 4815733971, Iran
*
Authors to whom correspondence should be addressed.
Foods 2020, 9(9), 1217; https://doi.org/10.3390/foods9091217
Received: 2 August 2020 / Revised: 21 August 2020 / Accepted: 28 August 2020 / Published: 2 September 2020
(This article belongs to the Special Issue Proteins and Bioactive Peptides in High Protein Content Foods)
In this study, orange seed proteins were hydrolyzed by Alcalase enzyme at different enzyme concentrations 1–3% (v/w) and hydrolysis times (2–5 h), to obtain bioactive peptides showing antioxidant, Angiotensin-converting enzyme (ACE) -inhibitory, and hypoglycemic activities. The highest biological activities (p < 0.05) were achieved by using a hydrolysis time of 5 h and an enzyme concentration of 2%. Orange seed protein hydrolysate (OSPH) was prepared under these conditions, and peptides were isolated and purified by using size-exclusion chromatography and high-performance liquid chromatography, respectively. The fractions that showed the highest biological activities were analyzed by mass spectrometry in tandem, and a total of 63 peptide sequences were found. Moreover, the effect of simulated gastrointestinal digestion on the bioactivity of the fractions was studied, and the novel peptide sequences generated were also identified. Overall, despite there being some differences in the profile of peptide sequences obtained, the main results showed non-significant differences in the analyzed bioactivities after simulated gastrointestinal digestion. View Full-Text
Keywords: orange seed; bioactive peptides; antioxidant; ACE-inhibitory; antidiabetic activity; gastrointestinal digestion orange seed; bioactive peptides; antioxidant; ACE-inhibitory; antidiabetic activity; gastrointestinal digestion
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MDPI and ACS Style

Mazloomi, S.N.; Mora, L.; Aristoy, M-C.; Mahoonak, A.S.; Ghorbani, M.; Houshmand, G.; Toldrá, F. Impact of Simulated Gastrointestinal Digestion on the Biological Activity of an Alcalase Hydrolysate of Orange Seed (Siavaraze, Citrus sinensis) by-Products. Foods 2020, 9, 1217. https://doi.org/10.3390/foods9091217

AMA Style

Mazloomi SN, Mora L, Aristoy M-C, Mahoonak AS, Ghorbani M, Houshmand G, Toldrá F. Impact of Simulated Gastrointestinal Digestion on the Biological Activity of an Alcalase Hydrolysate of Orange Seed (Siavaraze, Citrus sinensis) by-Products. Foods. 2020; 9(9):1217. https://doi.org/10.3390/foods9091217

Chicago/Turabian Style

Mazloomi, Seyadeh N.; Mora, Leticia; Aristoy, M-Concepción; Mahoonak, Alireza S.; Ghorbani, Mohammad; Houshmand, Gholamreza; Toldrá, Fidel. 2020. "Impact of Simulated Gastrointestinal Digestion on the Biological Activity of an Alcalase Hydrolysate of Orange Seed (Siavaraze, Citrus sinensis) by-Products" Foods 9, no. 9: 1217. https://doi.org/10.3390/foods9091217

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