Next Article in Journal
Experimental Detection of Particle Structures Detachment from a Stretchable Single Fiber during Multiple Consecutive Stretching Cycles
Next Article in Special Issue
Development of an Enzyme-Based Thin-Layer Chromatographic Assay for the Detection of Cyclooxygenase-2 Inhibitors
Previous Article in Journal
Microplastics and Heavy Metals Removal from Fresh Water and Wastewater Systems Using a Membrane
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Separations
Volume 9
Issue 7
10.3390/separations9070167
separations-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Analysis of Major Polyphenolic Compounds of Cydonia oblonga Miller (Quince) Fruit Extract by UPLC-MS/MS and Its Effect on Adipogenesis in 3T3-L1 Cells

by
Nawaz Khan
†,
Nulibiya Maihemuti
†,
Muhadaisi Nuer
,
Kayisaier Abudurousuli
,
Jimilihan Simayi
,
Ziruo Talihati
,
Mengyuan Han
,
Sendaer Hailati
,
Wenting Zhou
* and
Ainiwaer Wumaier
*
Department of Pharmacology, School of Pharmacy, Xinjiang Medical University, No. 393, Xinyi Road, Urumqi 830011, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Separations 2022, 9(7), 167; https://doi.org/10.3390/separations9070167
Submission received: 2 June 2022 / Revised: 24 June 2022 / Accepted: 27 June 2022 / Published: 1 July 2022
(This article belongs to the Special Issue Liquid Chromatography: Development of Separation Techniques)
Browse Figures
Versions Notes

Abstract

Cydonia oblonga miller (quince) plant serves as a potential folk medicine for treating hypertension and cardiovascular diseases in China. However, to the best of our knowledge, no study has been conducted on the polyphenolic profile and anti-adipogenic effect of quince fruit grown in China. In the current study, we aimed to investigate the quince fruit extract’s major phenolic compounds, evaluate their antioxidant activity, and examine their effect on adipogenesis in 3T3-L1 cells. A rapid and sensitive analytical method was established for the simultaneous determination of major polyphenolic compounds by using ultra-pressure liquid chromatography coupled with a triple quadrupole mass spectrometer (UPLC-MS/MS). Among the 10 compounds, the cryptochlorgenic acid was noticed as the most abundant compound of both purified (242.44 ± 0.73 µg/mg dw) and unpurified extract (3.37 ± 0.01 µg/mg dw) followed by quercetin 3-rutinoside and chlorogenic acid. Alternatively, both extracts possessed a high quantity of phenolic acids (purified extract = 483.10 ± 5.16 µg/mg dw and unpurified extract = 7.89 ± 0.02 µg/mg dw). The purified extract exhibited a strong antioxidant capacity (DPPH: EC50 = 3.316 µg/mL, ABTS: EC50 = 36.38 µg/mL) as compared to the unpurified extract. Additionally, our results also showed that the extract at 100 µg/mL significantly suppressed the preadipocyte differentiation and decreased the lipid droplets up to 69% in mature adipocytes. The present study highlights an accurate and fast detection method for quince fruit extract polyphenolic compounds with its antioxidant and antiadipogenic effects. The study also provides the necessary information for the rational development and utilization of quince fruit extract as a source of phytochemicals.
Keywords: UPLC-MS/MS; quince fruit extract; polyphenolic compounds; antioxidant; 3T3-L1 UPLC-MS/MS; quince fruit extract; polyphenolic compounds; antioxidant; 3T3-L1

Share and Cite

MDPI and ACS Style

Khan, N.; Maihemuti, N.; Nuer, M.; Abudurousuli, K.; Simayi, J.; Talihati, Z.; Han, M.; Hailati, S.; Zhou, W.; Wumaier, A. Analysis of Major Polyphenolic Compounds of Cydonia oblonga Miller (Quince) Fruit Extract by UPLC-MS/MS and Its Effect on Adipogenesis in 3T3-L1 Cells. Separations 2022, 9, 167. https://doi.org/10.3390/separations9070167

AMA Style

Khan N, Maihemuti N, Nuer M, Abudurousuli K, Simayi J, Talihati Z, Han M, Hailati S, Zhou W, Wumaier A. Analysis of Major Polyphenolic Compounds of Cydonia oblonga Miller (Quince) Fruit Extract by UPLC-MS/MS and Its Effect on Adipogenesis in 3T3-L1 Cells. Separations. 2022; 9(7):167. https://doi.org/10.3390/separations9070167

Chicago/Turabian Style

Khan, Nawaz, Nulibiya Maihemuti, Muhadaisi Nuer, Kayisaier Abudurousuli, Jimilihan Simayi, Ziruo Talihati, Mengyuan Han, Sendaer Hailati, Wenting Zhou, and Ainiwaer Wumaier. 2022. "Analysis of Major Polyphenolic Compounds of Cydonia oblonga Miller (Quince) Fruit Extract by UPLC-MS/MS and Its Effect on Adipogenesis in 3T3-L1 Cells" Separations 9, no. 7: 167. https://doi.org/10.3390/separations9070167

APA Style

Khan, N., Maihemuti, N., Nuer, M., Abudurousuli, K., Simayi, J., Talihati, Z., Han, M., Hailati, S., Zhou, W., & Wumaier, A. (2022). Analysis of Major Polyphenolic Compounds of Cydonia oblonga Miller (Quince) Fruit Extract by UPLC-MS/MS and Its Effect on Adipogenesis in 3T3-L1 Cells. Separations, 9(7), 167. https://doi.org/10.3390/separations9070167

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop