Anti-Hyperglycemic Effect of Magnesium-Enhanced Alkaline-Reduced Water on High Glucose-Induced Oxidative Stress in Renal Tubular Epithelial Cells
, Jayson M. Antonio 3, Ailyn Fadriquela 1,4, Thuy Thi Trinh 1,2, Md. Habibur Rahman 1,2, Kchorng Vira 1,2, Abdul-Nasir Sofian 1,2, Cheol-Su Kim 1 and Kyu-Jae Lee 1,2,*Round 1
Reviewer 1 Report
The manuscript ID processes-1704389 investigated the effect of magnesium-enhanced alkaline reduced water on anti-hyperglycemia in human kydney-2 cells.
The study is well designed, adequate methods were performed, the results are clearly presented and discussed according to the literature data.
Although, as the authors also concluded, those are results obtained in the in vitro experiments and more research is needed (specially in in vivo models), this study have promising and significant potential.
Author Response
Manuscript Title: Anti-hyperglycemic Effect of Magnesium-enhanced Alkaline Reduced Water on High Glucose-induced Oxidative Stress in Renal Tubular Epithelial Cells
Journal: Processes; Special Issue: Application and Development of Alkaline Ionized Water to Health Improvement.
Manuscript ID: Processes-1704389
Reviewer 1
The manuscript ID processes-1704389 investigated the effect of magnesium-enhanced alkaline reduced water on anti-hyperglycemia in human kydney-2 cells.
The study is well designed, adequate methods were performed, and the results are clearly presented and discussed according to the literature data.
Although, as the authors also concluded, those are results obtained in the in vitro experiments and more research is needed (especially in in vivo models), this study has promising and significant potential.
Author’s response: The authors really appreciate the reviewer for carefully checking our manuscript and giving us your valuable feedback. All the authors of the manuscript are thankful to reviewer for his/her valuable time and efforts.
Reviewer 2 Report
The work entitled "Anti-hyperglycemic Effect of Magnesium-enhanced Alkaline Reduced Water on High Glucose-induced Oxidative Stress in Renal Tubular Epithelial Cells" by Sharma et al. describes the use of magnesium to alkalinize the water, leading to the synthesis of MARW. The work is interesting and well written, the results are clearly explained and the methods are appropriate. I just have a minor concern about the figures 1 and 4. Their quality should be inproved because they are not so clear.
Author Response
Manuscript Title: Anti-hyperglycemic Effect of Magnesium-enhanced Alkaline Reduced Water on High Glucose-induced Oxidative Stress in Renal Tubular Epithelial Cells
Journal: Processes; Special Issue: Application and Development of Alkaline Ionized Water to Health Improvement.
Manuscript ID: Processes-1704389
Reviewer 2: Comments
The work entitled "Anti-hyperglycemic Effect of Magnesium-enhanced Alkaline Reduced Water on High Glucose-induced Oxidative Stress in Renal Tubular Epithelial Cells" by Sharma et al. describes the use of magnesium to alkalinize the water, leading to the synthesis of MARW. The work is interesting and well written, the results are clearly explained and the methods are appropriate. I just have a minor concern about the figures 1 and 4. Their quality should be improved because they are not so clear.
Author’s response: The authors really appreciate the reviewer for carefully checking our manuscript and giving us with your valuable feedback. According to reviewer suggestion authors have inserted Figure 1 and 4 with improved image quality in newly revised manuscript as shown below.
Author Response File: 
Author Response.docx
Reviewer 3 Report
The manuscript entitled “Anti-hyperglycemic Effect of Magnesium-enhanced Alkaline Reduced Water on High Glucose-induced Oxidative Stress in Renal Tubular Epithelial Cells” aimed to investigate the anti-hyperglycemic efficacy of magnesium-enhanced alkaline reduced water (MARW) in human kidney-2 epithelial (HK-2) cells. Oxidative stress, mitogen-activated protein kinase (MAPK) signalling, and fibrosis markers were evaluated in high glucose (HG)-induced HK-2 cells, followed by treatment with experimental water for 24 h. The obtained results showed that MARW rescued the vitality of HG-induced HK-2 cells, in contrast to that seen with other experimental waters. Besides, MARW maintained ROS, NO, CAT, GPx, hepatocyte growth factor, and glucose uptake in HG-induced HK-2 cells but not in tap water and mineral water. Moreover, MARW downregulated the expression of MAPK and various fibrosis-linked signalling proteins. Authors concluded that MARW protects HK-2 cells from the deleterious effects of HG by stabilizing antioxidant defences and signalling cascades related to metabolism, apoptosis and fibrosis.
Page 4 – what about the absorbance for the catalase measurement?
Page 5 – can you please reference the statement that caspase 3 is involved in programmed cell death or apoptosis
Minor remarks:
Page 2 – change to “Additionally, ROS act as secondary messengers that activates various signaling cascades…”
Page 5 – place 50 in subscript (IC50)
I would suggest to use larger font in figures x and y axes since they are hard to read. In general, the numbers and letters in all figures are very small so could be enlarged.
Author Response
Manuscript Title: Anti-hyperglycemic Effect of Magnesium-enhanced Alkaline Reduced Water on High Glucose-induced Oxidative Stress in Renal Tubular Epithelial Cells
Journal: Processes; Special Issue: Application and Development of Alkaline Ionized Water to Health Improvement.
Manuscript ID: Processes-1704389
Reviewer 3: Comments
The manuscript entitled “Anti-hyperglycemic Effect of Magnesium-enhanced Alkaline Reduced Water on High Glucose-induced Oxidative Stress in Renal Tubular Epithelial Cells” aimed to investigate the anti-hyperglycemic efficacy of magnesium-enhanced alkaline reduced water (MARW) in human kidney-2 epithelial (HK-2) cells. Oxidative stress, mitogen-activated protein kinase (MAPK) signalling, and fibrosis markers were evaluated in high glucose (HG)-induced HK-2 cells, followed by treatment with experimental water for 24 h. The obtained results showed that MARW rescued the vitality of HG-induced HK-2 cells, in contrast to that seen with other experimental waters. Besides, MARW maintained ROS, NO, CAT, GPx, hepatocyte growth factor, and glucose uptake in HG-induced HK-2 cells but not in tap water and mineral water. Moreover, MARW down regulated the expression of MAPK and various fibrosis-linked signalling proteins. Authors concluded that MARW protects HK-2 cells from the deleterious effects of HG by stabilizing antioxidant defenses and signalling cascades related to metabolism, apoptosis and fibrosis.
Page 4 – what about the absorbance for the catalase measurement?
Author’s response: The authors really appreciate the reviewer for carefully checking our manuscript. Regarding reviewer question about the absorbance measurement for the catalase measurement, it was measured at 560 nm and we have included this information in our newly revised manuscript (Line no: 223).
Page 5 – can you please reference the statement that caspase 3 is involved in programmed cell death or apoptosis
Author’s response: The authors really appreciate the reviewer for carefully checking our manuscript. Following your suggestion, authors have inserted the related reference related to caspase 3 in newly revised manuscript (Line no: 304-305)
Cited Reference:
- Porter, A.G.; Jänicke, R.U. Emerging roles of caspase-3 in apoptosis. Cell. Death. Differ.1999; 6, 99-104.
Minor remarks:
Page 2 – change to “Additionally, ROS act as secondary messengers that activates various signaling cascades…”
Author’s response: The authors really appreciate the reviewer for carefully checking our manuscript. Following your suggestion, authors have made necessary in newly revised manuscript (Line no: 61-62).
Page 5 – place 50 in subscript (IC50)
Author’s response: The authors really appreciate the reviewer for carefully checking our manuscript. Following your suggestion, authors have change IC50 to IC50 in newly revised manuscript (Line no: 297).
I would suggest to use larger font in figures x and y axes since they are hard to read. In general, the numbers and letters in all figures are very small so could be enlarged.
Author’s response: The authors really appreciate the reviewer for carefully checking our manuscript. Following reviewer suggestion, authors have revised all the figures and used larger font size in x and y axis in our newly revised manuscript.
Author Response File: Author Response.docx
