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Brief Report

Shotgun Metagenomic Sequencing Identifies Dysbiosis in Triplet Sibling with Gastrointestinal Symptoms and ASD

1
Progenabiome™ Ventura Clinical Trials, 1835 Knoll Dr, Ventura, CA 93003, USA
2
Department of Internal Medicine, Wake Forest School of Medicine, Medical Center Blvd, Winston Salem, NC 27157, USA
3
Wake Forest Institute for Regenerative Medicine, 391 Technology Way, Winston Salem, NC 27101, USA
*
Author to whom correspondence should be addressed.
Children 2020, 7(12), 255; https://doi.org/10.3390/children7120255
Received: 22 October 2020 / Revised: 18 November 2020 / Accepted: 23 November 2020 / Published: 25 November 2020
The gut microbiome profile of a child with autism spectrum disorder (ASD) and co-occurring gastrointestinal (GI) symptoms was compared to that of her healthy triplet siblings to determine if she exhibited intestinal dysbiosis. Shotgun metagenomic sequencing was performed in individual fecal samples, and relative microbial abundance and diversity was determined. Microbial diversity was lower in sibling #3, coupled with a higher Bacteroidetes/Firmicutes ratio, a lower relative abundance of Actinobacteria, and an increased relative abundance of Proteobacteria. Our findings are suggestive of gut dysbiosis in a child with ASD and co-occurring GI symptoms, compared to her two healthy triplet siblings. View Full-Text
Keywords: autism spectrum disorder; gastrointestinal; gut microbiome; metagenomic sequencing autism spectrum disorder; gastrointestinal; gut microbiome; metagenomic sequencing
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MDPI and ACS Style

Hazan, S.; Spradling-Reeves, K.D.; Papoutsis, A.; Walker, S.J. Shotgun Metagenomic Sequencing Identifies Dysbiosis in Triplet Sibling with Gastrointestinal Symptoms and ASD. Children 2020, 7, 255. https://doi.org/10.3390/children7120255

AMA Style

Hazan S, Spradling-Reeves KD, Papoutsis A, Walker SJ. Shotgun Metagenomic Sequencing Identifies Dysbiosis in Triplet Sibling with Gastrointestinal Symptoms and ASD. Children. 2020; 7(12):255. https://doi.org/10.3390/children7120255

Chicago/Turabian Style

Hazan, Sabine, Kimberly D. Spradling-Reeves, Andreas Papoutsis, and Stephen J. Walker. 2020. "Shotgun Metagenomic Sequencing Identifies Dysbiosis in Triplet Sibling with Gastrointestinal Symptoms and ASD" Children 7, no. 12: 255. https://doi.org/10.3390/children7120255

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