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Biomedicines 2015, 3(1), 89-109;

HGF Modulates Actin Cytoskeleton Remodeling and Contraction in Testicular Myoid Cells

Department of Anatomy, Histology, Forensic Medicine and Orthopedics, Section of Histology and Embryology, Sapienza University of Rome, Via A. Scarpa 16, 00161 Rome, Italy
Department of Experimental Medicine, Histology and Embryology Laboratory, School of Medicine, Second University of Naples, Via Luciano Armanni 5, 80138 Naples, Italy
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Zimmer Yitzhak
Received: 29 September 2014 / Revised: 10 November 2014 / Accepted: 21 January 2015 / Published: 28 January 2015
(This article belongs to the Special Issue New aspects of the Hepatocyte Growth Factor/c-Met System)
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The presence of the HGF/Met system in the testicular myoid cells was first discovered by our group. However, the physiological role of this pathway remains poorly understood. We previously reported that HGF increases uPA secretion and TGF-β activation in cultured tubular fragments and that HGF is maximally expressed at Stages VII–VIII of the seminiferous epithelium cycle, when myoid cell contraction occurs. It is well known that the HGF/Met pathway is involved in cytoskeletal remodeling; moreover, the interaction of uPA with its receptor, uPAR, as well as the activation of TGF-β have been reported to be related to the actin cytoskeleton contractility of smooth muscle cells. Herein, we report that HGF induces actin cytoskeleton remodeling in vitro in isolated myoid cells and myoid cell contraction in cultured seminiferous tubules. To better understand these phenomena, we evaluated: (1) the regulation of the uPA machinery in isolated myoid cells after HGF administration; and (2) the effect of uPA or Met inhibition on HGF-treated tubular fragments. Because uPA activates latent TGF-β, the secretion of this factor was also evaluated. We found that both uPA and TGF-β activation increase after HGF administration. In testicular tubular fragments, HGF-induced TGF-β activation and myoid cell contraction are abrogated by uPA or Met inhibitor administration. View Full-Text
Keywords: myoid cells; HGF/c-Met; uPA; actin cytoskeleton; TGF-β myoid cells; HGF/c-Met; uPA; actin cytoskeleton; TGF-β

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Catizone, A.; Ricci, G.; Caruso, M.; Galdieri, M.; Scheri, K.C.; Di Paolo, V.; Canipari, R. HGF Modulates Actin Cytoskeleton Remodeling and Contraction in Testicular Myoid Cells. Biomedicines 2015, 3, 89-109.

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