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Small Noncoding RNAs in Reproduction and Infertility
 
 
Article

Testicular “Inherited Metabolic Memory” of Ancestral High-Fat Diet Is Associated with Sperm sncRNA Content

1
Departamento de Anatomia, e UMIB—Unidade Multidisciplinar de Investigação em Biomedicina, ICBAS—Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, 4050-313 Porto, Portugal
2
Laboratory for Integrative and Translational Research in Population Health (ITR), University of Porto, 4050-600 Porto, Portugal
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Integrative Physiology and Pharmacology Unit, Institute of Biomedicine, University of Turku, Kiinamyllynkatu 10, 20520 Turku, Finland
4
Polytechnic Institute of Guarda, School of Health, 6300-035 Guarda, Portugal
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Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6200-506 Covilhã, Portugal
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NOVA Medical School, New University Lisbon, 1250-189 Lisbon, Portugal
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Associação Protetora dos Diabéticos de Portugal (APDP), 1250-189 Lisbon, Portugal
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Centre for Obesity Research, Department of Medicine, University College London (UCL), London WC1E 6JF, UK
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National Institute for Health Research, Biomedical Research Centre, University College London Hospital (UCLH), London W1T 7DN, UK
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Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, 17003 Girona, Spain
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Unit of Cell Biology, Department of Biology, Faculty of Sciences, University of Girona, 17003 Girona, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Giuseppina Catanzaro
Biomedicines 2022, 10(4), 909; https://doi.org/10.3390/biomedicines10040909
Received: 9 March 2022 / Revised: 8 April 2022 / Accepted: 8 April 2022 / Published: 15 April 2022
(This article belongs to the Special Issue Non-coding RNAs in Health and Disease)
Excessive adiposity caused by high-fat diets (HFDs) is associated with testicular metabolic and functional abnormalities up to grand-offspring, but the mechanisms of this epigenetic inheritance are unclear. Here we describe an association of sperm small non-coding RNA (sncRNA) with testicular “inherited metabolic memory” of ancestral HFD, using a transgenerational rodent model. Male founders were fed a standard chow for 200 days (CTRL), HFD for 200 days (HFD), or standard chow for 60 days followed by HFD for 140 days (HFDt). The male offspring and grand-offspring were fed standard chow for 200 days. The sncRNA sequencing from epidydimal spermatozoa revealed signatures associated with testicular metabolic plasticity in HFD-exposed mice and in the unexposed progeny. Sperm tRNA-derived RNA (tsRNA) and repeat-derived small RNA (repRNA) content were specially affected by HFDt and in the offspring of HFD and HFDt mice. The grand-offspring of HFD and HFDt mice showed lower sperm counts than CTRL descendants, whereas the sperm miRNA content was affected. Although the causality between sperm sncRNAs content and transgenerational epigenetic inheritance of HFD-related traits remains elusive, our results suggest that sperm sncRNA content is influenced by ancestral exposure to HFD, contributing to the sperm epigenome up to the grand-offspring. View Full-Text
Keywords: high-fat diet; sperm parameters; sncRNA; paternal epigenetic inheritance; inherited metabolic memory high-fat diet; sperm parameters; sncRNA; paternal epigenetic inheritance; inherited metabolic memory
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MDPI and ACS Style

Crisóstomo, L.; Bourgery, M.; Rato, L.; Raposo, J.F.; Batterham, R.L.; Kotaja, N.; Alves, M.G. Testicular “Inherited Metabolic Memory” of Ancestral High-Fat Diet Is Associated with Sperm sncRNA Content. Biomedicines 2022, 10, 909. https://doi.org/10.3390/biomedicines10040909

AMA Style

Crisóstomo L, Bourgery M, Rato L, Raposo JF, Batterham RL, Kotaja N, Alves MG. Testicular “Inherited Metabolic Memory” of Ancestral High-Fat Diet Is Associated with Sperm sncRNA Content. Biomedicines. 2022; 10(4):909. https://doi.org/10.3390/biomedicines10040909

Chicago/Turabian Style

Crisóstomo, Luís, Matthieu Bourgery, Luís Rato, João F. Raposo, Rachel L. Batterham, Noora Kotaja, and Marco G. Alves. 2022. "Testicular “Inherited Metabolic Memory” of Ancestral High-Fat Diet Is Associated with Sperm sncRNA Content" Biomedicines 10, no. 4: 909. https://doi.org/10.3390/biomedicines10040909

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