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Open AccessArticle

UHPLC-Q-TOF/MS-Based Metabolomics Approach Reveals the Antifungal Potential of Pinocembroside against Citrus Green Mold Phytopathogen

by Chuying Chen 1, Nan Cai 1, Jinyin Chen 1,2,* and Chunpeng Wan 1,*
1
Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits & Vegetables, Collaborative Innovation Center of Postharvest Key Technology and Quality Safety of Fruits & Vegetables in Jiangxi Province, Jiangxi Agricultural University, Nanchang 330045, China
2
Pingxiang University, Pingxiang 337055, China
*
Authors to whom correspondence should be addressed.
Plants 2020, 9(1), 17; https://doi.org/10.3390/plants9010017
Received: 28 November 2019 / Revised: 16 December 2019 / Accepted: 20 December 2019 / Published: 22 December 2019
(This article belongs to the Special Issue Advances in Citrus Research)
Pinocembroside (PiCB) isolated from Ficus hirta Vahl. fruit was studied herein with the aim to find the potential mechanism for significant inhibition of growth of Penicillium digitatum, a causative pathogen of citrus green mold disease. PiCB substantially inhibited mycelial growth of P. digitatum, with the observed half maximal effective concentration (EC50), minimum inhibitory concentration (MIC), and minimum fungicidal concentration (MFC) of 120.3, 200, and 400 mg/L, respectively. Moreover, PiCB altered hyphal morphology and cellular morphology by breaking and shrinking of mycelia, decomposing cell walls, cytoplasmic inclusions. In addition to, a non-targeted metabolomics analysis by UHPLC-Q-TOF/MS was also performed, which revealed that PiCB treatment notably disrupted the metabolisms of amino acids, lipids, fatty acids, TCA, and ribonucleic acids, thereby contributing to membrane peroxidation. Current findings provide a new perception into the antifungal mechanism of PiCB treatment in inhibiting P. digitatum growth through membrane peroxidation. View Full-Text
Keywords: Penicillium digitatum; pinocembroside; green mold; antifungal mechanism; membrane peroxidation Penicillium digitatum; pinocembroside; green mold; antifungal mechanism; membrane peroxidation
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Chen, C.; Cai, N.; Chen, J.; Wan, C. UHPLC-Q-TOF/MS-Based Metabolomics Approach Reveals the Antifungal Potential of Pinocembroside against Citrus Green Mold Phytopathogen. Plants 2020, 9, 17.

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