Expression and Ion Transport Activity of Rice OsHKT1;1 Variants
Abstract
:1. Introduction
2. Results
2.1. Isolation and Characterization of OsHKT1;1 cDNAs
2.2. Expression Analyses of OsHKT1;1 in Shoots and Roots
2.3. Ion Transport Activity of OsHKT1;1-FL and Its Variants
3. Discussion
4. Materials and Methods
4.1. Plant Material and Growth Condition
4.2. Extraction of DNA and RNA, and cDNA Synthesis
4.3. Secondly Structure Analysis
4.4. Expression Analysis
4.5. Expression in Xenopus laevis (X. leavis) Oocytes
4.6. Electrophysiology
4.7. Statistics
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
- Munns, R.; James, R.A.; Xu, B.; Athman, A.; Conn, S.J.; Jordans, C.; Byrt, C.S.; Hare, R.A.; Tyerman, S.D.; Tester, M.; et al. Wheat grain yield on saline soils is improved by an ancestral Na+ transporter gene. Nat. Biotechnol. 2012, 30, 360–364. [Google Scholar] [CrossRef] [PubMed]
- Munns, R.; Tester, M. Mechanisms of salinity tolerance. Annu. Rev. Plant Biol. 2008, 59, 651–681. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Ismail, A.M.; Horie, T. Genomics, physiology, and molecular breeding approaches for improving salt tolerance. Annu. Rev. Plant Biol. 2017, 68, 405–434. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- United States Department of Agriculture. Bibliography on Salt Tolerance. Fibres, Grains and Special Crops; Brown, G.E., Jr., Ed.; Salinity Laboratory United States Department of Agriculture Research Service: Riverside, CA, USA. Available online: http://www.Ars.Usda.Gov/services/docs.Htm?Docid=8908 (accessed on 20 August 2016).
- Horie, T.; Hauser, F.; Schroeder, J.I. HKT transporter-mediated salinity resistance mechanisms in Arabidopsis and monocot crop plants. Trends Plant Sci. 2009, 14, 660–668. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Rubio, F.; Nieves-Cordones, M.; Horie, T.; Shabala, S. Doing ‘business as usual’ comes with a cost: Evaluating energy cost of maintaining plant intracellular K+ homeostasis under saline conditions. New Phytol. 2019. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Rubio, F.; Gassmann, W.; Schroeder, J.I. Sodium-driven potassium uptake by the plant potassium transporter HKT1 and mutations conferring salt tolerance. Science 1995, 270, 1660–1663. [Google Scholar] [CrossRef]
- Platten, J.D.; Cotsaftis, O.; Berthomieu, P.; Bohnert, H.; Davenport, R.J.; Fairbairn, D.J.; Horie, T.; Leigh, R.A.; Lin, H.X.; Luan, S.; et al. Nomenclature for HKT transporters, key determinants of plant salinity tolerance. Trends Plant Sci. 2006, 11, 372–374. [Google Scholar] [CrossRef]
- Hauser, F.; Horie, T. A conserved primary salt tolerance mechanism mediated by HKT transporters: A mechanism for sodium exclusion and maintenance of high K+/Na+ ratio in leaves during salinity stress. Plant Cell Environ. 2010, 33, 552–565. [Google Scholar] [CrossRef]
- Gassmann, W.; Rubio, F.; Schroeder, J.I. Alkali cation selectivity of the wheat root high-affinity potassium transporter HKT1. Plant J. 1996, 10, 869–882. [Google Scholar] [CrossRef]
- Uozumi, N.; Kim, E.J.; Rubio, F.; Yamaguchi, T.; Muto, S.; Tsuboi, A.; Bakker, E.P.; Nakamura, T.; Schroeder, J.I. The Arabidopsis HKT1 gene homolog mediates inward Na+ currents in Xenopus laevis oocytes and Na+ uptake in Saccharomyces cerevisiae. Plant Physiol. 2000, 122, 1249–1259. [Google Scholar] [CrossRef] [Green Version]
- Xue, S.; Yao, X.; Luo, W.; Jha, D.; Tester, M.; Horie, T.; Schroeder, J.I. AtHKT1;1 Mediates Nernstian Sodium Channel Transport Properties in Arabidopsis Root Stellar Cells. PLoS ONE 2011, 6, e24725. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Garciadeblás, B.; Senn, M.E.; Bañuelos, M.A.; Rodríguez-Navarro, A. Sodium transport and HKT transporters: The rice model. Plant J. 2003, 34, 788–801. [Google Scholar] [CrossRef] [PubMed]
- Ren, Z.H.; Gao, J.P.; Li, L.G.; Cai, X.L.; Huang, W.; Chao, D.Y.; Zhu, M.Z.; Wang, Z.Y.; Luan, S.; Lin, H.X. A rice quantitative trait locus for salt tolerance encodes a sodium transporter. Nat. Genet. 2005, 37, 1141–1146. [Google Scholar] [CrossRef] [PubMed]
- Jabnoune, M.; Espeout, S.; Mieulet, D.; Fizames, C.; Verdeil, J.L.; Conéjéro, G.; Rodríguez-Navarro, A.; Sentenac, H.; Guiderdoni, E.; Abdelly, C.; et al. Diversity in expression patterns and functional properties in the rice HKT transporter family. Plant Physiol. 2009, 150, 1955–1971. [Google Scholar] [CrossRef] [Green Version]
- Rosas-Santiago, P.; Lagunas-Gómez, D.; Barkla, B.J.; Vera-Estrella, R.; Lalonde, S.; Jones, A.; Frommer, W.B.; Zimmermannova, O.; Sychrová, H.; Pantoja, O. Identification of rice cornichon as a possible cargo receptor for the Golgi-localized sodium transporter OsHKT1;3. J. Exp. Bot. 2015, 66, 2733–2748. [Google Scholar] [CrossRef]
- Suzuki, K.; Costa, A.; Nakayama, H.; Katsuhara, M.; Shinmyo, A.; Horie, T. OsHKT2;2/1‑mediated Na+ influx over K+ uptake in roots potentially increases toxic Na+ accumulation in a salt-tolerant landrace of rice Nona Bokra upon salinity stress. J. Plant Res. 2016, 129, 67–77. [Google Scholar] [CrossRef]
- Sunarpi, H.T.; Horie, T.; Motoda, J.; Kubo, M.; Yang, H.; Yoda, K.; Horie, R.; Chan, W.Y.; Leung, H.Y.; Hattori, K.; et al. Enhanced salt tolerance mediated by AtHKT1 transporter-induced Na unloading from xylem vessels to xylem parenchyma cells. Plant J. 2005, 44, 928–938. [Google Scholar] [CrossRef]
- Møller, I.S.; Gilliham, M.; Jha, D.; Mayo, G.M.; Roy, S.J.; Coates, J.C.; Haseloff, J.; Tester, M. Shoot Na+ exclusion and increased salinity tolerance engineered by cell type-specific alteration of Na+ transport in Arabidopsis. Plant Cell 2009, 21, 2163–2178. [Google Scholar] [CrossRef] [Green Version]
- Kobayashi, N.I.; Yamaji, N.; Yamamoto, H.; Okubo, K.; Ueno, H.; Costa, A.; Tanoi, K.; Matsumura, H.; Fujii-Kashino, M.; Horiuchi, T.; et al. OsHKT1;5 mediates Na+ exclusion in the vasculature to protect leaf blades and reproductive tissues from salt toxicity in rice. Plant J. 2017, 91, 657–670. [Google Scholar] [CrossRef] [Green Version]
- Wang, R.; Jing, W.; Xiao, L.; Jin, Y.; Shen, L.; Zhang, W. The Rice High-Affinity Potassium Transporter1;1 Is Involved in Salt Tolerance and Regulated by an MYB-Type Transcription Factor. Plant Physiol. 2015, 168, 1076–1090. [Google Scholar] [CrossRef] [Green Version]
- Takagi, H.; Tamiru, M.; Abe, A.; Yoshida, K.; Uemura, A.; Yaegashi, H.; Obara, T.; Oikawa, K.; Utsushi, H.; Kanzaki, E.; et al. MutMap accelerates breeding of a salt-tolerant rice cultivar. Nat. Biotechnol. 2015, 33, 445–449. [Google Scholar] [CrossRef] [PubMed]
- Campbell, M.T.; Bandillo, N.; Al Shiblawi, F.R.A.; Sharma, S.; Liu, K.; Du, Q.; Schmitz, A.J.; Zhang, C.; Very, A.A.; Lorenz, A.J.; et al. Allelic variants of OsHKT1;1 underlie the divergence between indica and japonica subspecies of rice (Oryza sativa) for root sodium content. PLoS Genet. 2017, 13, e1006823. [Google Scholar] [CrossRef] [PubMed]
- Qiu, L.; Wu, D.; Ali, S.; Cai, S.; Dai, F.; Jin, X.; Wu, F.; Zhang, G. Evaluation of salinity tolerance and analysis of allelic function of HvHKT1 and HvHKT2 in Tibetan wild barley. Theor. Appl. Genet. 2011, 122, 695–703. [Google Scholar] [CrossRef] [PubMed]
- Han, Y.; Yin, S.; Huang, L.; Wu, X.; Zeng, J.; Liu, X.; Qiu, L.; Munns, R.; Chen, Z.-H.; Zhang, G. A Sodium Transporter HvHKT1;1 Confers Salt Tolerance in Barley via Regulating Tissue and Cell Ion Homeostasis. Plant Cell Physiol. 2018, 59, 1976–1989. [Google Scholar] [CrossRef]
- Xu, M.; Chen, C.; Cai, H.; Wu, L. Overexpression of PeHKT1;1 Improves Salt Tolerance in Populus. Genes 2018, 9, 475. [Google Scholar] [CrossRef] [Green Version]
- Deinlein, U.; Stephan, A.B.; Horie, T.; Luo, W.; Xu, G.; Schroeder, J.I. Plant salt-tolerance mechanisms. Trends Plant Sci. 2014, 19, 371–379. [Google Scholar] [CrossRef] [Green Version]
- Berthomieu, P.; Conéjéro, G.; Nublat, A.; Brackenbury, W.J.; Lambert, C.; Savio, C.; Uozumi, N.; Oiki, S.; Yamada, K.; Cellier, F.; et al. Functional analysis of AtHKT1 in Arabidopsis shows that Na+ recirculation by the phloem is crucial for salt tolerance. EMBO J. 2003, 22, 2004–2014. [Google Scholar] [CrossRef] [Green Version]
- Munns, R.; Rebetzke, G.J.; Husain, S.; James, R.A.; Hare, R.A. Genetic control of sodium exclusion in durum wheat. Aust. J. Agric. Res. 2003, 54, 627–635. [Google Scholar] [CrossRef]
- James, R.A.; Davenport, R.J.; Munns, R. Physiological characterization of two genes for Na+ exclusion in durum wheat, Nax1 and Nax2. Plant Physiol. 2006, 142, 1537–1547. [Google Scholar] [CrossRef] [Green Version]
- Byrt, C.S.; Platten, J.D.; Spielmeyer, W.; James, R.A.; Lagudah, E.S.; Dennis, E.S.; Tester, M.; Munns, R. HKT1;5-like cation transporters linked to Na+ exclusion loci in wheat, Nax2 and Kna1. Plant Physiol. 2007, 143, 1918–1928. [Google Scholar] [CrossRef] [Green Version]
- Tounsi, S.; Amar, B.S.; Masmoudi, K.; Sentenac, H.; Brini, F.; Véry, A.A. Characterization of Two HKT1;4 Transporters from Triticum monococcum to Elucidate the Determinants of the Wheat Salt Tolerance Nax1 QTL. Plant Cell Physiol. 2016, 57, 2047–2057. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Katsuhara, M.; Rhee, J.; Sugimoto, G.; Chung, G.C. Early response in water relations influenced by NaCl reflects tolerance or sensitivity of barley plants to salinity stress via aquaporins. Soil Sci. Plant Nutr. 2010, 57, 50–60. [Google Scholar] [CrossRef]
- Mahdieh, M.; Mostajeran, A.; Horie, T.; Katsuhara, M. Drought stress alters water relations and expression of PIP-type aquaporin genes in Nicotiana tabacum plants. Plant Cell Physiol. 2008, 49, 801–813. [Google Scholar] [CrossRef] [PubMed]
- Katsuhara, M.; Akiyama, Y.; Koshio, K.; Shibasaka, M.; Kasamo, K. Functional analysis of water channels in barley roots. Plant Cell Physiol. 2002, 43, 885–893. [Google Scholar] [CrossRef] [PubMed]
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Share and Cite
Imran, S.; Horie, T.; Katsuhara, M. Expression and Ion Transport Activity of Rice OsHKT1;1 Variants. Plants 2020, 9, 16. https://doi.org/10.3390/plants9010016
Imran S, Horie T, Katsuhara M. Expression and Ion Transport Activity of Rice OsHKT1;1 Variants. Plants. 2020; 9(1):16. https://doi.org/10.3390/plants9010016
Chicago/Turabian StyleImran, Shahin, Tomoaki Horie, and Maki Katsuhara. 2020. "Expression and Ion Transport Activity of Rice OsHKT1;1 Variants" Plants 9, no. 1: 16. https://doi.org/10.3390/plants9010016