Next Article in Journal
UHPLC-Q-TOF/MS-Based Metabolomics Approach Reveals the Antifungal Potential of Pinocembroside against Citrus Green Mold Phytopathogen
Previous Article in Journal
Copy Number Variation of Transposable Elements in Thinopyrum intermedium and Its Diploid Relative Species
Open AccessFeature PaperArticle

Expression and Ion Transport Activity of Rice OsHKT1;1 Variants

Institute of Plant Science and Resources, Okayama University, 2-20-1, Chuo, Kurashiki 710-0046, Japan
Division of Applied Biology, Faculty of the Textile Science and Technology, Shinshu University, 3-15-1, Tokida, Ueda 386-8567, Japan
Authors to whom correspondence should be addressed.
Plants 2020, 9(1), 16;
Received: 21 November 2019 / Revised: 11 December 2019 / Accepted: 19 December 2019 / Published: 21 December 2019
(This article belongs to the Special Issue Plant Membrane Transporters)
OsHKT1;1 in rice, belongs to the high-affinity K+ Transporter family, has been found to be involved in salt tolerance. OsHKT1;1 in japonica rice (Nipponbare) produces mRNA variants, but their functions remain elusive. In salt tolerant rice, Pokkali, eight OsHKT1;1 variants (V1-V8) were identified in addition to the full-length OsHKT1;1 (FL) cDNA. Absolute quantification by qPCR revealed that accumulation of OsHKT1;1-FL mRNA is minor in contrast to that of OsHKT1;1-V1, -V2, -V4, and -V7 mRNAs, all of which are predominant in shoots, while only V1 and V7 mRNAs are predominant in roots. Two electrode voltage clamp (TEVC) experiments using Xenopus laevis oocytes revealed that oocytes-expressing OsHKT1;1-FL from Pokkali exhibited inward-rectified currents in the presence of 96 mM Na+ as reported previously. Further TEVC analyses indicated that six of eight OsHKT1;1 variants elicited currents in a Na+ or a K+ bath solution. OsHKT1;1-V6 exhibited a similar inward rectification to the FL protein. Contrastingly, however, the rests mediated bidirectional currents in both Na+ and K+ bath solutions. These data suggest possibilities that novel mechanisms regulating the transport activity of OsHKT1;1 might exist, and that OsHKT1;1 variants might also carry out distinct physiological roles either independently or in combination with OsHKT1;1-FL. View Full-Text
Keywords: HKT; rice; salt tolerance; Na+ transport; K+ transport; mRNA variants HKT; rice; salt tolerance; Na+ transport; K+ transport; mRNA variants
Show Figures

Figure 1

MDPI and ACS Style

Imran, S.; Horie, T.; Katsuhara, M. Expression and Ion Transport Activity of Rice OsHKT1;1 Variants. Plants 2020, 9, 16.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop