Hemeroby is an integrated indicator used to measure the impact and degree of all human interventions on ecological components or ecosystems. The constant exploitation of resources is a strong interference of human beings to the natural environment. With the depletion of non-renewable resources, some cities with resource exploitation as their main industry—“resource-based cities”—are facing great development pressure. In order to quantify the impact of human disturbance on the natural environment and provide some scientific support for policy makers of the resource-based city, we used remote sensing images and landscape pattern metrics, introduced the synthetic hemeroby index model and analyzed the relationship between human disturbance and landscape pattern during 1990–2017. The results showed that: (1) The hemeroby in Daqing continued to rise during 1990–2017, and the main factor was the continuous expansion of the construction land and the reclamation of farmland. (2) In the areas with different hemeroby, there were significant differences in landscape pattern. In the areas with high-level hemeroby, the heterogeneity of landscape pattern was low, the aggregation among patches was high, and the shape of patches was regular, whereas the landscape pattern in the areas with medium-level hemeroby was just opposite. Although the heterogeneity of landscape pattern and the aggregation among patches were high in the areas with low-level hemeroby, the complexity of landscape was low and the shape of patches was regular. (3) In the temporal dimension, the increase of hemeroby contributed to the complexity of patch shape, the decrease of the aggregation among patches, and the fragmentation of landscape pattern. In the spatial dimension, the response in landscape pattern to human disturbance was relatively insensitive in the areas with low-level hemeroby, and this response was basically same in the high-level hemeroby and the whole study areas.
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