Protective Effects of Sesamin against UVB-Induced Skin Inflammation and Photodamage In Vitro and In Vivo
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Cell Culture and UV Irradiation
2.3. Detection of ROS Production
2.4. Western Blot Analysis
2.5. Measuring Total Collagen Synthesis in Fibroblasts
2.6. Immunofluorescence Staining
2.7. Effect of Sesamin Treatment on Photodamage in Hairless Mice
2.7.1. Animals
2.7.2. Experimental Design
2.7.3. Detection of Erythema (a* Value) and Transepidermal Water Loss of Mice Skin
2.7.4. Immunohistological Analysis
2.8. Statistical Analysis
3. Results
3.1. Sesamin Did Not Cause Cytotoxicity in Hs68 Cells
3.2. Sesamin Inhibited Intracellular ROS Formation in Hs68 Cells
3.3. Effects and Mechanisms of Sesamin on Skin Photodamage
3.3.1. Sesamin Inhibited UVB-Induced Overexpression of MMPs and Increased TIMP Expression
3.3.2. Sesamin Inhibited UVB-Induced Overexpression of c-Jun/p-c-Jun
3.3.3. Sesamin Inhibited the Upregulation of MAP Kinases Induced by UVB Irradiation
3.3.4. Sesamin Modulated the Expressions of Smad3 and Smad7
3.3.5. Sesamin Attenuated UVB-Inhibited Total Collagen Biosynthesis
3.4. Anti-Inflammatory Effect of Sesamin
3.4.1. Sesamin Ameliorated UVB-Induced Overexpression of i-NOS and COX-2
3.4.2. Sesamin Reduced COX-2 Expression by Inhibiting MAP Kinase Expression
3.4.3. Sesamin Inhibited UVB-Induced NF-κB Activation
3.5. Sesamin Protected Mouse Skin from UVB-Irradiation-Induced Damage
3.5.1. Sesamin Reduced UVB-Induced Skin Erythema and Damage
3.5.2. Sesamin Reduced UVB-Induced Wrinkle Formation
3.5.3. Sesamin Reduced UVB-Induced Epidermal Hyperplasia and Restored Collagen Content
3.5.4. Sesamin Inhibited Photodamage-Related Protein Levels in UVB-Irradiated Mouse Skin
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Group | Wrinkle Score (10th Week) |
---|---|
Normal mice | 0.7 ± 1.2 a |
UVB-irradiated mice | 4.5 ± 1.9 b |
UVB-irradiated mice + vehicle | 5.3 ± 1.2 b |
UVB-irradiated mice + sesamin (50 μM) | 1.3 ± 1.6 a,c |
UVB-irradiated mice + sesamin (200 μM) | 1.3 ± 1.0 a,c |
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Lin, T.-Y.; Wu, P.-Y.; Hou, C.-W.; Chien, T.-Y.; Chang, Q.-X.; Wen, K.-C.; Lin, C.-Y.; Chiang, H.-M. Protective Effects of Sesamin against UVB-Induced Skin Inflammation and Photodamage In Vitro and In Vivo. Biomolecules 2019, 9, 479. https://doi.org/10.3390/biom9090479
Lin T-Y, Wu P-Y, Hou C-W, Chien T-Y, Chang Q-X, Wen K-C, Lin C-Y, Chiang H-M. Protective Effects of Sesamin against UVB-Induced Skin Inflammation and Photodamage In Vitro and In Vivo. Biomolecules. 2019; 9(9):479. https://doi.org/10.3390/biom9090479
Chicago/Turabian StyleLin, Tzu-Yu, Po-Yuan Wu, Chien-Wei Hou, Ting-Yi Chien, Qiao-Xin Chang, Kuo-Ching Wen, Chien-Yih Lin, and Hsiu-Mei Chiang. 2019. "Protective Effects of Sesamin against UVB-Induced Skin Inflammation and Photodamage In Vitro and In Vivo" Biomolecules 9, no. 9: 479. https://doi.org/10.3390/biom9090479