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Biomolecules 2018, 8(2), 37; https://doi.org/10.3390/biom8020037

Simple Analysis of Lipid Inhibition Activity on an Adipocyte Micro-Cell Pattern Chip

1
Biotechnology Research Division, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 29 Geumgu-gil, Jeongeup-si, Jeonbuk-do 56212, Korea
2
Department of Chemical Engineering, Chungnam National Univiersity, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea
3
Department of Chemical and Biomolecular Engineering, Chonnam National University, 50, Daehak-ro, Yeosu-si, Jeollanam-do 59626, Korea
*
Authors to whom correspondence should be addressed.
Received: 5 April 2018 / Revised: 25 May 2018 / Accepted: 31 May 2018 / Published: 4 June 2018
(This article belongs to the Special Issue Obesity and Hormones)
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Abstract

Polydimethyl-siloxane (PDMS) is often applied to fabricate cell chips. In this study, we fabricated an adipocyte microcell pattern chips using PDMS to analyze the inhibition activity of lipid droplets in mouse embryo fibroblast cells (3T3-L1) with anti-obesity agents. To form the PDMS based micropattern, we applied the micro-contact printing technique using PDMS micro-stamps that had been fabricated by conventional soft lithography. This PDMS micro-pattern enabled the selective growth of 3T3-L1 cells onto the specific region by preventing cell adhesion on the PDMS region. It then allowed growth of the 3T3-L1 cells in the chip for 10 days and confirmed that lipid droplets were formed in the 3T3-L1 cells. After treatment of orlistat and quercetin were treated in an adipocyte micro-cell pattern chip with 3T3-L1 cells for six days, we found that orlistat and quercetin exhibited fat inhibition capacities of 19.3% and 24.4% from 0.2 μM of lipid droplets in 3T3-L1 cells. In addition, we conducted a direct quantitative analysis of 3T3-L1 cell differentiation using Oil Red O staining. In conclusion, PDMS-based adipocyte micro-cell pattern chips may contribute to the development of novel bioactive compounds. View Full-Text
Keywords: micro-contacting; orlistat; quercetin; 3T3-L1 cell; anti-obesity agents micro-contacting; orlistat; quercetin; 3T3-L1 cell; anti-obesity agents
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Kim, G.Y.; Yeom, S.-J.; Jang, S.-C.; Lee, C.-S.; Roh, C.; Jeong, H.-H. Simple Analysis of Lipid Inhibition Activity on an Adipocyte Micro-Cell Pattern Chip. Biomolecules 2018, 8, 37.

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