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Open AccessArticle

Tracking Bacterial Spoilage in Cosmetics by a New Bioanalytical Approach: API-SPME-GC-MS to Monitor MVOCs

1
CRETUS Institute, Department of Analytical Chemistry, Nutrition and Food Science, Universidade de Santiago de Compostela, E-15782 Santiago de Compostela, Spain
2
BIOMIG, Polusa Hospital, E-27001 Lugo, Spain
*
Author to whom correspondence should be addressed.
Cosmetics 2020, 7(2), 38; https://doi.org/10.3390/cosmetics7020038
Received: 4 May 2020 / Revised: 19 May 2020 / Accepted: 20 May 2020 / Published: 21 May 2020
(This article belongs to the Special Issue Feature Papers in Cosmetics in 2020)
The main goal of this work was the use of the powerful solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) technique to unequivocally identify microbial volatile organic compounds (MVOCs) derived from the enzymatic activity produced during metabolic processes using analytical profile index (API) biochemical tests. Three bacteria were selected for this study: Escherichia coli, Proteus mirabilis, and Pseudomonas aeruginosa. They were inoculated and incubated to both API components and real cosmetics, as well as to a mixture of them. Specific MVOCs were successfully identified as biomarkers for each one of the studied microorganisms: Indole and 2-nitrophenol as Escherichia coli markers, 2-undecanone and phenylethyl alcohol as Proteus mirabilis-specific markers, and 1-undecene and 2′-aminoacetophenone as Pseudomonas aeruginosa ones. In addition, a high number of MVOCs were identified as general markers of bacterial presence. The results revealed that the MVOCs’ formation is highly subtract dependent. Therefore, the ultimate and most challenging objective is to establish a relationship between the identified MVOCs and the original compound present in the substrate. This work establishes the design and development of this original approach, and its practical application to the control of microbial contamination in real cosmetic samples. View Full-Text
Keywords: microbial volatile organic compounds; bacterial contamination; consumers’ health; analytical profile index; solid-phase microextraction; gas chromatography-mass spectrometry microbial volatile organic compounds; bacterial contamination; consumers’ health; analytical profile index; solid-phase microextraction; gas chromatography-mass spectrometry
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MDPI and ACS Style

Celeiro, M.; Varela, E.; Rodriguez, R.; Penedo, M.; Lores, M. Tracking Bacterial Spoilage in Cosmetics by a New Bioanalytical Approach: API-SPME-GC-MS to Monitor MVOCs. Cosmetics 2020, 7, 38.

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