Physicochemical Characteristics, Fatty Acid Profile, and In Vitro Antioxidant Activity Evaluation of Sacha Inchi Seed Oil from Indonesia

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

On line 20: cancel the common after vitamin A, D, E;

Line 26 write g and not gr, cm3 write the Number 3 smaller

Line 27 write g and not grams

Line 76 cancel One of  a Square bracket [[6-8]

Line 85 don't use Round parentheses but use a common when you write: " skin care products, especially as antiaging"

Line 124 Rewrite all the sentence

Line 403 vitamin A, D and E,  correct dan in and

Line 449 before the Square bracket, delete a space

Line 576 delete figure 1, the mechanism of DPPH Is well known 

Some qualitative analyses were performed with ancient methods now superseded by more modern equipment. The paper doesn't the cosmetic application with no efficacy test related. So this isn't the journal appropriate for this work. The paper is rejected 

Author Response

First of all, we deeply appreciate your helpful comments. Our replies to the reviewer’s inquiries and revised points are as follows. Please confirm the relevant parts in the revised manuscript.

Replies to Reviewer #1

Comments 1

1. On line 20: cancel the common after vitamin A, D, E.

Reply to comment 1

Thank you for your valuable comment. We have fixed the sentence in line 20 by cancelling the typical sentence after vitamins A, D, and E. This revision was added to the manuscript in Line 20 and marked with red-colored text.

Comments 2

2. Line 26 write g and not gr, cm3 write the Number 3 smaller.

Reply to comment 2

Thank you for your valuable comment. We have fixed the error of writing gr to g and the number 3 in cm3 in uppercase. These revisions were added to the manuscript in Line 26-27 and marked with red-colored text.

Comments 3

3. Line 27 write g and not grams

Reply to comment 3

Thank you for your valuable comments. We have fixed the error of writing grams in the refractive index unit, which should be unitless. This revision was added to the manuscript in Line 27 and marked with red-colored text.

Comments 4

4. Line 76 cancel One of a Square bracket [[6-8]

Reply to comment 4

Thank you for your valuable comments. We have fixed the error of writing square brackets, which should only be one square bracket. This revision was added to the manuscript in Line 75, previously in Line 76 and marked with red-colored text.

Comments 5

5. Line 85 don't use round parentheses but use a common when you write: "skin care products, especially as antiaging"

Reply to comment 5

Thank you for your valuable comments. We have modified the error of writing round brackets in the sentence "skin care products, (especially as antiaging)" to "skin care products, especially as antiaging". This revision was added to the manuscript in Line 84, previously in Line 85 and marked with red-colored text.

Comments 6

6. Line 124 Rewrite all the sentence

Reply to comment 6

Thank you for your valuable comments. We have modified the sentence in Line 154, previously in Line 124. This revision was added to the manuscript in Line 154 and marked with red-colored text.

Comments 7

7. Line 403 vitamin A, D and E, correct dan in and

Reply to comment 7

Thank you for your valuable comments. We have fixed the error of writing "dan" in line 433, previously in Line 403. It should be "Vitamin A, D, and E". This revision was added to the manuscript in Line 410 and marked with red-colored text.

Comments 8

8. Line 449 before the Square bracket, delete a space

Reply to comment 8

Thank you for your valuable comments. We have fixed the error of writing the space before the square brackets in Line 475, previously Line 449. This revision was added to the manuscript in Line 475 and marked with red-colored text.

Comments 9

9. Line 576 delete figure 1, the mechanism of DPPH Is well known 

Reply to comment 9

Thank you for your valuable comments. We have reviewed Figure 1, and the DPPH mechanism is already available in Lines 617–619 and 621–622. However, this figure in Line 628, previously Line 576, was added as a brief overview of the mechanism of flavonoids against DPPH free radicals.

Comments 10

10. Some qualitative analyses were performed with ancient methods now superseded by more modern equipment. The paper doesn't the cosmetic application with no efficacy test related. So this isn't the journal appropriate for this work. The paper is rejected.

Reply to comment 10

Thank you for your valuable comment. We welcome suggestions and introductions from reviewers regarding this research. Since the use of sacha inchi as anti-aging is novel, this research is intended to be the preliminary study for further investigation. Therefore, this study only evaluates the effectiveness of sacha inchi using DPPH scavenging assay.

Reviewer 2 Report

Comments and Suggestions for Authors

1.     In the Introduction section, the authors claimed that “Sacha inchi seed oil is a plant that can act as a practical antioxidant component.” What is the innovation of the present manuscript?

2.     In the Introduction, please talk about the prevention of ageing with the cosmetic treatments.

3.     In the Discussion section, the authors should discuss the advantages and disadvantages of Sacha inchi seed oil with other cosmetic treatments.

4.     The possible action mechanisms of Sacha inchi seed oil should be discussed further.

Comments on the Quality of English Language

None

Author Response

Reviewer #2

First of all, we deeply appreciate your helpful comments. Our replies to the reviewer’s inquiries and revised points are as follows. Please confirm the relevant parts in the revised manuscript.

Replies to Reviewer #2

Comments 1

1. In the Introduction section, the authors claimed that “Sacha inchi seed oil is a plant that can act as a practical antioxidant component.” What is the innovation of the present manuscript?

Reply to comment 1

Thank you for your valuable comments. This text innovation is the result of research on sacha inchi seed oil originating from Indonesia. Sacha inchi is a plant originating from Peru which has many benefits in the pharmaceutical sector, especially in the cosmetic sector, so this plant is starting to be widely cultivated, one of which is in Indonesia. In order to guarantee that the sacha inchi seed oil produced in Indonesia adheres to the requirements set by its country of origin, Peru, this research carried out tests on physicochemical parameters, fatty acid content, vitamins (A, D, and E), and antioxidant activity. In research articles that have been published regarding sacha inchi oil, no testing for vitamin D has been found, so this research article is the first to review this matter. Apart from that, this research also tested for microbial and heavy metal contamination, which were not found in previous research articles. Likewise, research articles on the topic of sacha inchi oil originating from Indonesia are still minimal, and no research articles have yet been published. Therefore, this study aims to fill the gap in the existing literature by investigating the vitamin D content, microbial contamination, and heavy metals in sacha inchi oil. Additionally, by focusing on the Indonesian context, this research will contribute valuable insights into the potential benefits and safety of sacha inchi oil from this region.

Comments 2

2. In the Introduction, please talk about the prevention of ageing with the cosmetic treatments.

Reply to comment 2

Thank you for your valuable comment. We have added some information about preventing ageing with cosmetic treatments in the introduction. This revision was added to the text in Line 87-109 and is marked with green-colored text.

Comments 3

3. In the Discussion section, the authors should discuss the advantages and disadvantages ofSacha inchi seed oil with other cosmetic treatments.

Reply to comment 3

Thank you for your valuable comments. We have added some information regarding the advantages and disadvantages of sacha inchi seed oil with other cosmetic treatments in the discussion section. This revision was added to the text in Line 500-510 and is marked with green-colored text.

Comments 4

4. The possible action mechanisms of sacha inchi seed oilshould be discussed further.

Reply to comment 4

Thank you for your valuable comments. We have added some information regarding the working mechanism of sacha inchi seed oil in the discussion section. This revision was added to the text on Line 511-525 and is marked with green-colored text.

Reviewer 3 Report

Comments and Suggestions for Authors

More experiments should be conducted to ensure the usefulness of oil in the cosmetics.

Author Response

Reviewer #3

First of all, we deeply appreciate your helpful comments. Our replies to the reviewer’s inquiries and revised points are as follows. Please confirm the relevant parts in the revised manuscript.

Replies to Reviewer #3

Comments 1

1. More experiments should be conducted to ensure the usefulness of oil in the cosmetics 

Reply to comment 1

Thank you for your valuable comment. We welcome suggestions and introductions from reviewers regarding this research. Since the use of sacha inchi as anti-aging is novel, this research is intended to be the preliminary study for further investigation. Therefore, this study only evaluates the effectiveness of sacha inchi using DPPH scavenging assay. Furthermore, the study centers on the process of standardization and early characterization to comprehend the constituents, physicochemical attributes, safety concerns related to contaminants, and preliminary antioxidant effects. With its high omegas and vitamin content, we can measure and direct where its pharmacological and cosmetology uses are. The study primarily emphasizes the identification and safety assessment of sacha inchi seed oil, with the aim of providing valuable information for its application in cosmetic manufacture and formulation.

Round 2

Reviewer 1 Report

Comments and Suggestions for Authors

I appreciate the authors’ comments and accept the paper with only one revision:

I only suggest in the Conclusion to add this phrase: “This is a study primarily on the identification and safety assessment of sacha inchi seed oil, with the aim of providing valuable information for its application in cosmetic manufacture and formulation”, to justify the cosmetic use indicated in the paper.

Author Response

Reviewer #1

First of all, we deeply appreciate your helpful comments. Our replies to the reviewer’s inquiries and revised points are as follows. Please confirm the relevant parts in the revised manuscript.

Replies to Reviewer #1

Comments 1

I only suggest in the Conclusion to add this phrase: “This is a study primarily on the identification and safety assessment of sacha inchi seed oil, with the aim of providing valuable information for its application in cosmetic manufacture and formulation”, to justify the cosmetic use indicated in the paper.

Reply to comment 1

Thank you for your valuable suggestions. We welcome suggestions from reviewers regarding this research. We have modified the conclusion section to include the reviewer's suggested phrase. This revision was added to the manuscript in Line 568-570 and marked with red-colored text.

 

Reviewer 2 Report

Comments and Suggestions for Authors

None

Comments on the Quality of English Language

None

Author Response

We appreciate your acceptance of our journal. Thank you for your acceptance.