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Antibiotics
Volume 9
Issue 8
10.3390/antibiotics9080474
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Article

Contact Effect of a Methylobacterium sp. Extract on Biofilm of a Mycobacterium chimaera Strain Isolated from a 3T Heater-Cooler System

by
Inés Pradal
1,
Jaime Esteban
1,*,
Arancha Mediero
2,
Marta García-Coca
3 and
John Jairo Aguilera-Correa
1
1
Clinical Microbiology Department, IIS-Fundación Jiménez Díaz, UAM, 28040 Madrid, Spain
2
Bone and Joint Unit, IIS-Fundación Jiménez Díaz, UAM, 28040 Madrid, Spain
3
Clinical Microbiology Department, Quironsalud-Madrid University Hospital, 28223 Pozuelo de Alarcón, Spain
*
Author to whom correspondence should be addressed.
Antibiotics 2020, 9(8), 474; https://doi.org/10.3390/antibiotics9080474
Submission received: 22 June 2020 / Revised: 22 July 2020 / Accepted: 29 July 2020 / Published: 3 August 2020
(This article belongs to the Special Issue Mycobacterial Infections and Therapy)
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Abstract

Mycobacterium chimaera is an opportunistic slowly growing non-tuberculous mycobacteriumof increasing importance due to the outbreak of cases associated with contaminated 3T heater-cooler device (HCD) extracorporeal membrane oxygenator (ECMO). The aim of this study was to evaluate the effect of pre-treating a surface with a Methylobacterium sp. CECT 7180 extract to inhibit the M. chimaera ECMO biofilm as well as of the treatment after different dehydration times. Surface adherence, biofilm formation and treatment effect were evaluated by estimating colony-forming units (CFU) per square centimeter and characterizing the amount of covered surface area, thickness, cell viability, and presence of intrinsic autofluorescence at different times using confocal laser scanning microscopy and image analysis. We found that exposing a surface to the Methylobacterium sp. CECT 7180 extract inhibited M. chimaera ECMO biofilm development. This effect could be result of the effect of Methylobacterium proteins, such as DNaK, trigger factor, and xanthine oxidase. In conclusion, exposing a surface to the Methylobacteriumsp. extract inhibits M. chimaera ECMO biofilm development. Furthermore, this extract could be used as a pre-treatment prior to disinfection protocols for equipment contaminated with mycobacteria after dehydration for at least 96 h.
Keywords: Mycobacterium chimaera; 3T HCD; biofilm; Methylobacterium; antibiofilm effect Mycobacterium chimaera; 3T HCD; biofilm; Methylobacterium; antibiofilm effect

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MDPI and ACS Style

Pradal, I.; Esteban, J.; Mediero, A.; García-Coca, M.; Aguilera-Correa, J.J. Contact Effect of a Methylobacterium sp. Extract on Biofilm of a Mycobacterium chimaera Strain Isolated from a 3T Heater-Cooler System. Antibiotics 2020, 9, 474. https://doi.org/10.3390/antibiotics9080474

AMA Style

Pradal I, Esteban J, Mediero A, García-Coca M, Aguilera-Correa JJ. Contact Effect of a Methylobacterium sp. Extract on Biofilm of a Mycobacterium chimaera Strain Isolated from a 3T Heater-Cooler System. Antibiotics. 2020; 9(8):474. https://doi.org/10.3390/antibiotics9080474

Chicago/Turabian Style

Pradal, Inés, Jaime Esteban, Arancha Mediero, Marta García-Coca, and John Jairo Aguilera-Correa. 2020. "Contact Effect of a Methylobacterium sp. Extract on Biofilm of a Mycobacterium chimaera Strain Isolated from a 3T Heater-Cooler System" Antibiotics 9, no. 8: 474. https://doi.org/10.3390/antibiotics9080474

APA Style

Pradal, I., Esteban, J., Mediero, A., García-Coca, M., & Aguilera-Correa, J. J. (2020). Contact Effect of a Methylobacterium sp. Extract on Biofilm of a Mycobacterium chimaera Strain Isolated from a 3T Heater-Cooler System. Antibiotics, 9(8), 474. https://doi.org/10.3390/antibiotics9080474

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