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Antibiotics
Volume 12
Issue 2
10.3390/antibiotics12020205
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Article

Molecular Basis of Methicillin and Vancomycin Resistance in Staphylococcus aureus from Cattle, Sheep Carcasses and Slaughterhouse Workers

by
Hanan A. Zaher
1,
Shimaa El Baz
2,
Abdulaziz S. Alothaim
3,
Sulaiman A. Alsalamah
4,
Mohammed Ibrahim Alghonaim
4,
Abdullah S. Alawam
4 and
Mostafa M. Eraqi
3,5,*
1
Food Hygiene and Control Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt
2
Department of Hygiene and Zoonoses, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt
3
Department of Biology, College of Science in Zulfi, Majmaah University, Majmaah 11952, Saudi Arabia
4
Department of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University, Riyadh 11623, Saudi Arabia
5
Microbiology and Immunology Department, Veterinary Research Institute, National Research Centre, Dokki, Giza 12622, Egypt
*
Author to whom correspondence should be addressed.
Antibiotics 2023, 12(2), 205; https://doi.org/10.3390/antibiotics12020205
Submission received: 21 December 2022 / Revised: 13 January 2023 / Accepted: 14 January 2023 / Published: 18 January 2023
(This article belongs to the Special Issue Molecular Determinants of Antibiotic Resistance in Methicillin-Resistant Staphylococcus aureus (MRSA))
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Abstract

Staphylococcus aureus (S. aureus) is a serious infection-causing pathogen in humans and animal. In particular, methicillin-resistant S. aureus (MRSA) is considered one of the major life-threatening pathogens due to its rapid resistance to several antibiotics in clinical practice. MRSA strains have recently been isolated in a number of animals utilized in food production processes, and these species are thought to be the important sources of the spread of infection and disease in both humans and animals. The main objective of the current study was to assess the prevalence of drug-resistant S. aureus, particularly vancomycin-resistant S. aureus (VRSA) and MRSA, by molecular methods. To address this issue, a total of three hundred samples (200 meat samples from cattle and sheep carcasses (100 of each), 50 hand swabs, and 50 stool samples from abattoir workers) were obtained from slaughterhouses in Egypt provinces. In total, 19% S. aureus was isolated by standard culture techniques, and the antibiotic resistance was confirmed genotypically by amplification nucA gen. Characteristic resistance genes were identified by PCR with incidence of 31.5%, 19.3%, 8.7%, and 7% for the mecA, VanA, ermA, and tet L genes, respectively, while the aac6-aph gene was not found in any of the isolates. In this study, the virulence genes responsible for S. aureus’ resistance to antibiotics had the highest potential for infection or disease transmission to animal carcasses, slaughterhouse workers, and meat products.
Keywords: Staphylococcus aureus; MRSA; VRSA; cattle; sheep Staphylococcus aureus; MRSA; VRSA; cattle; sheep

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MDPI and ACS Style

Zaher, H.A.; El Baz, S.; Alothaim, A.S.; Alsalamah, S.A.; Alghonaim, M.I.; Alawam, A.S.; Eraqi, M.M. Molecular Basis of Methicillin and Vancomycin Resistance in Staphylococcus aureus from Cattle, Sheep Carcasses and Slaughterhouse Workers. Antibiotics 2023, 12, 205. https://doi.org/10.3390/antibiotics12020205

AMA Style

Zaher HA, El Baz S, Alothaim AS, Alsalamah SA, Alghonaim MI, Alawam AS, Eraqi MM. Molecular Basis of Methicillin and Vancomycin Resistance in Staphylococcus aureus from Cattle, Sheep Carcasses and Slaughterhouse Workers. Antibiotics. 2023; 12(2):205. https://doi.org/10.3390/antibiotics12020205

Chicago/Turabian Style

Zaher, Hanan A., Shimaa El Baz, Abdulaziz S. Alothaim, Sulaiman A. Alsalamah, Mohammed Ibrahim Alghonaim, Abdullah S. Alawam, and Mostafa M. Eraqi. 2023. "Molecular Basis of Methicillin and Vancomycin Resistance in Staphylococcus aureus from Cattle, Sheep Carcasses and Slaughterhouse Workers" Antibiotics 12, no. 2: 205. https://doi.org/10.3390/antibiotics12020205

APA Style

Zaher, H. A., El Baz, S., Alothaim, A. S., Alsalamah, S. A., Alghonaim, M. I., Alawam, A. S., & Eraqi, M. M. (2023). Molecular Basis of Methicillin and Vancomycin Resistance in Staphylococcus aureus from Cattle, Sheep Carcasses and Slaughterhouse Workers. Antibiotics, 12(2), 205. https://doi.org/10.3390/antibiotics12020205

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