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Open AccessArticle

A DNA-Based Assay for Digoxin Detection

Interdisciplinary Nanoscience Center (iNANO) and Department of Chemistry, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark
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Biosensors 2018, 8(1), 19; https://doi.org/10.3390/bios8010019
Received: 6 February 2018 / Revised: 27 February 2018 / Accepted: 1 March 2018 / Published: 6 March 2018
(This article belongs to the Special Issue FRET-Based Biosensors)
The most common method for quantifying small-molecule drugs in blood samples is by liquid chromatography in combination with mass spectrometry. Few immuno-based assays are available for the detection of small-molecule drugs in blood. Here we report on a homogeneous assay that enables detection of the concentration of digoxin spiked into in a plasma sample. The assay is based on a shift in the equilibrium of a DNA strand displacement competition reaction, and can be performed in 30 min for concentrations above 10 nM. The equilibrium shift occurs upon binding of anti-digoxigenin antibody. As a model, the assay provides a potential alternative to current small-molecule detection methods used for therapeutic drug monitoring. View Full-Text
Keywords: digoxin detection; homogeneous assay; DNA nanotechnology; DNA strand displacement competition reaction; detection of small molecules; detection in plasma digoxin detection; homogeneous assay; DNA nanotechnology; DNA strand displacement competition reaction; detection of small molecules; detection in plasma
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Kjelstrup, M.V.; Nielsen, L.D.F.; Hansen-Bruhn, M.; Gothelf, K.V. A DNA-Based Assay for Digoxin Detection. Biosensors 2018, 8, 19.

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