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Open AccessArticle

Amperometric Self-Referencing Ceramic Based Microelectrode Arrays for D-Serine Detection

1
Institute of Experimental and Clinical Pharmacology and Toxicology, University of Lübeck, Ratzeburger Allee 160, 23562 Lübeck, Germany
2
The Department of Pharmaceutical Biosciences, Uppsala University, 75124 Uppsala, Sweden
3
The Department of Physiology and Pharmacology, Karolinska Institutet, 17177 Stockholm, Sweden
4
CenMeT, University of Kentucky, Lexington, 40506 KY, USA
5
Quanteon LLC, Nicholasville, 40356 KY, USA
*
Author to whom correspondence should be addressed.
Biosensors 2018, 8(1), 20; https://doi.org/10.3390/bios8010020
Received: 1 February 2018 / Revised: 15 February 2018 / Accepted: 2 March 2018 / Published: 6 March 2018
(This article belongs to the Special Issue Implantable Biosensors for in vivo Detection and Measurement)
D-serine is the major D-amino acid in the mammalian central nervous system. As the dominant co-agonist of the endogenous synaptic NMDA receptor, D-serine plays a role in synaptic plasticity, learning, and memory. Alterations in D-serine are linked to neuropsychiatric disorders including schizophrenia. Thus, it is of increasing interest to monitor the concentration of D-serine in vivo as a relevant player in dynamic neuron-glia network activity. Here we present a procedure for amperometric detection of D-serine with self-referencing ceramic-based microelectrode arrays (MEAs) coated with D-amino acid oxidase from the yeast Rhodotorula gracilis (RgDAAO). We demonstrate in vitro D-serine recordings with a mean sensitivity of 8.61 ± 0.83 pA/µM to D-serine, a limit of detection (LOD) of 0.17 ± 0.01 µM, and a selectivity ratio of 80:1 or greater for D-serine over ascorbic acid (mean ± SEM; n = 12) that can be used for freely moving studies. View Full-Text
Keywords: D-serine; biosensor; microelectrode array; amperometry; self-referencing D-serine; biosensor; microelectrode array; amperometry; self-referencing
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MDPI and ACS Style

Campos-Beltrán, D.; Konradsson-Geuken, Å.; Quintero, J.E.; Marshall, L. Amperometric Self-Referencing Ceramic Based Microelectrode Arrays for D-Serine Detection. Biosensors 2018, 8, 20. https://doi.org/10.3390/bios8010020

AMA Style

Campos-Beltrán D, Konradsson-Geuken Å, Quintero JE, Marshall L. Amperometric Self-Referencing Ceramic Based Microelectrode Arrays for D-Serine Detection. Biosensors. 2018; 8(1):20. https://doi.org/10.3390/bios8010020

Chicago/Turabian Style

Campos-Beltrán, Diana; Konradsson-Geuken, Åsa; Quintero, Jorge E.; Marshall, Lisa. 2018. "Amperometric Self-Referencing Ceramic Based Microelectrode Arrays for D-Serine Detection" Biosensors 8, no. 1: 20. https://doi.org/10.3390/bios8010020

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