Next Article in Journal
Fabrication of Highly Conductive Porous Cellulose/PEDOT:PSS Nanocomposite Paper via Post-Treatment
Next Article in Special Issue
A Suite of Therapeutically-Inspired Nucleic Acid Logic Systems for Conditional Generation of Single-Stranded and Double-Stranded Oligonucleotides
Previous Article in Journal / Special Issue
Smart-Responsive Nucleic Acid Nanoparticles (NANPs) with the Potential to Modulate Immune Behavior
Article Menu
Issue 4 (April) cover image

Export Article

Open AccessFeature PaperArticle

First Step Towards Larger DNA-Based Assemblies of Fluorescent Silver Nanoclusters: Template Design and Detailed Characterization of Optical Properties

1
Department of Physics, University of Nebraska Omaha, Omaha, NE 68182, USA
2
Nanoimaging Core Facility at the University of Nebraska Medical Center, Omaha, NE 68198, USA
3
Nanoscale Science Program, Department of Chemistry, University of North Carolina at Charlotte, Charlotte, NC 28223, USA
*
Author to whom correspondence should be addressed.
Nanomaterials 2019, 9(4), 613; https://doi.org/10.3390/nano9040613
Received: 19 March 2019 / Revised: 7 April 2019 / Accepted: 12 April 2019 / Published: 13 April 2019
  |  
PDF [2278 KB, uploaded 13 April 2019]
  |  

Abstract

Besides being a passive carrier of genetic information, DNA can also serve as an architecture template for the synthesis of novel fluorescent nanomaterials that are arranged in a highly organized network of functional entities such as fluorescent silver nanoclusters (AgNCs). Only a few atoms in size, the properties of AgNCs can be tuned using a variety of templating DNA sequences, overhangs, and neighboring duplex regions. In this study, we explore the properties of AgNCs manufactured on a short DNA sequence—an individual element designed for a construction of a larger DNA-based functional assembly. The effects of close proximity of the double-stranded DNA, the directionality of templating single-stranded sequence, and conformational heterogeneity of the template are presented. We observe differences between designs containing the same AgNC templating sequence—twelve consecutive cytosines, (dC)12. AgNCs synthesized on a single “basic” templating element, (dC)12, emit in “red”. The addition of double-stranded DNA core, required for the larger assemblies, changes optical properties of the silver nanoclusters by adding a new population of clusters emitting in “green”. A new population of “blue” emitting clusters forms only when ssDNA templating sequence is placed on the 5′ end of the double-stranded core. We also compare properties of silver nanoclusters, which were incorporated into a dimeric structure—a first step towards a larger assembly. View Full-Text
Keywords: silver nanoclusters; fluorescence; i-motif DNA; cytosine rich sequences silver nanoclusters; fluorescence; i-motif DNA; cytosine rich sequences
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Yourston, L.E.; Lushnikov, A.Y.; Shevchenko, O.A.; Afonin, K.A.; Krasnoslobodtsev, A.V. First Step Towards Larger DNA-Based Assemblies of Fluorescent Silver Nanoclusters: Template Design and Detailed Characterization of Optical Properties. Nanomaterials 2019, 9, 613.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Nanomaterials EISSN 2079-4991 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top