Elevated Serum 25-Hydroxyvitamin D Levels Are Associated with Improved Bone Formation and Micro-Structural Measures in Elderly Hip Fracture Patients
Abstract
:1. Introduction
2. Experimental Section
2.1. Bone and Serum Specimens
2.2. Serum Analyses
2.3. Bone Biopsy Prepareation
2.4. Bone Biopsy Micro-Computed Tomography
2.5. Bone Biopsy Histology
2.6. Rodiographic Cortical Bone Analyses
2.7. Bone mRNA Analyses
2.8. Statistical Analyses
3. Results
3.1. Biochemical Analyses
3.2. Relationship between Serum Vitamin D Levels and Bone Structure and Histomorphometric Measures
3.3. Contribution of CYP27B1 and CYP24A1 mRNA Expression in Bone to Histomorphometric Measures
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
Appendix A
25(OH)D Interval nmol/L (Number of Patients) | Median 25(OH)D Levels (nmol/L) | Test Statistics | p Value (vs. >70 nmol/L) | Adjusted p Value a (vs. >70 nmol/L) |
---|---|---|---|---|
10–30 (21) | 6.17 | −50.31 | <0.001 | <0.001 |
30–50 (18) | 7.16 | −34.77 | <0.001 | <0.001 |
50–70 (20) | 8.40 | −17.17 | 0.021 | 0.12 |
>70 (29) | 9.70 |
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Median (IQ Range) | Range | |
---|---|---|
Age, years | 86.5 (80.3–90.3) | 63.0–102.7 |
eGFR, mL/min/1.73 m2 | 70 (47–84) | 11.0–90+ |
Ionized Ca, mmol/L | 1.19 (1.15–1.23) | 1.03–1.37 |
25(OH)D, nmol/L | 52.7 (30.4–73.4) | 10–225 |
1,25(OH)2D, pmol/L | 93.8 (67.1–129.8) | 18.4–229 |
PTH, pmol/L | 4.9 (3.28–9.43) | 1.1–32.7 |
Osteocalcin, nmol/L | 2.41 (1.21–3.24) | 0.33–21.8 |
BS-ALP, µg/L | 13.3 (9.78–17.23) | 3.45–36.30 |
Dependent Variable | Independent Variables | Standardized Coefficients (beta) | p-Value |
---|---|---|---|
Serum 1,25(OH)2D | 25(OH)D | 0.332 | <0.001 |
+PTH | 0.272 | 0.006 | |
+eGFR | 0.439 | <0.001 | |
+Constant | +0.187 | 0.992 | |
Combined R2 = 0.235 | <0.001 |
Dependent Variable | Independent Variables | Standardized Coefficients (beta) | p-Value |
---|---|---|---|
MWT | 25(OH)D | 0.745 | <0.001 |
+1,25(OH)2D | +0.095 | 0.190 (NS) | |
+5.4 | |||
Adjusted R2 = 0.602 | p < 0.001 |
Dependent Variable | Independent Variables | Standardized Coefficients (beta) | p-Value |
---|---|---|---|
MWT | 25(OH)D | 0.743 | <0.001 |
+CYP27B1 | +0.043 | 0.655 (NS) | |
+CYP24A1 | +0.064 | 0.504 (NS) | |
+5.1 | |||
Adjusted R2 = 0.54 | p < 0.001 |
Dependent Variable | Independent Variables | Standardized Coefficients (beta) | p-Value |
---|---|---|---|
BS/BV | 25(OH)D | −0.294 | 0.012 |
+CYP27B1 | −0.208 | 0.115 | |
+CYP24A1 | +0.306 | 0.021 | |
+27.5 | |||
Adjusted R2 = 0.104 | 0.016 |
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Sharma, D.K.; Sawyer, R.K.; Robertson, T.S.; Stamenkov, R.; Solomon, L.B.; Atkins, G.J.; Clifton, P.M.; Morris, H.A.; Anderson, P.H. Elevated Serum 25-Hydroxyvitamin D Levels Are Associated with Improved Bone Formation and Micro-Structural Measures in Elderly Hip Fracture Patients. J. Clin. Med. 2019, 8, 1988. https://doi.org/10.3390/jcm8111988
Sharma DK, Sawyer RK, Robertson TS, Stamenkov R, Solomon LB, Atkins GJ, Clifton PM, Morris HA, Anderson PH. Elevated Serum 25-Hydroxyvitamin D Levels Are Associated with Improved Bone Formation and Micro-Structural Measures in Elderly Hip Fracture Patients. Journal of Clinical Medicine. 2019; 8(11):1988. https://doi.org/10.3390/jcm8111988
Chicago/Turabian StyleSharma, Deepti K., Rebecca K. Sawyer, Thomas S. Robertson, Roumen Stamenkov, Lucian B. Solomon, Gerald J. Atkins, Peter M. Clifton, Howard A. Morris, and Paul H. Anderson. 2019. "Elevated Serum 25-Hydroxyvitamin D Levels Are Associated with Improved Bone Formation and Micro-Structural Measures in Elderly Hip Fracture Patients" Journal of Clinical Medicine 8, no. 11: 1988. https://doi.org/10.3390/jcm8111988