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Article

Antioxidative Effects of Ascorbic Acid and Astaxanthin on ARPE-19 Cells in an Oxidative Stress Model

1
Interdisciplinary Program in Stem Cell Biology, Graduate School of Medicine, Seoul National University, Seoul 03080, Korea
2
Department of Ophthalmology, Seoul National University Hospital Biomedical Research Institute, Seoul 03080, Korea
3
Department of Ophthalmology, Seoul National University College of Medicine, Seoul National University Hospital, Seoul 03080, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Antioxidants 2020, 9(9), 833; https://doi.org/10.3390/antiox9090833
Received: 31 July 2020 / Revised: 3 September 2020 / Accepted: 3 September 2020 / Published: 6 September 2020
(This article belongs to the Special Issue Oxidative Stress in Diabetic Retinopathy)
Oxidative stress has been implicated as critical pathogenic factors contributing to the etiology of diabetic retinopathy and other retinal diseases. This study investigated antioxidative effect of ascorbic acid and astaxanthin on ARPE-19 cells within an oxidative stress model induced by common biological sources of reactive oxygen species (ROS). Hydrogen peroxide (H2O2) at concentrations of 0.1–0.8 mM and 20–100 mJ/cm2 of ultraviolet B (UVB) were treated to ARPE-19 cells. Cell viability and intracellular ROS level changes were measured. With the sublethal and lethal dose of each inducers, 0–750 μM of ascorbic acid and 0–40 μM of astaxanthin were treated to examine antioxidative effect on the model. Ascorbic acid at concentrations of 500 and 750 μM increased the cell viability not only in the UVB model but also in the H2O2 model, but 20 and 40 μM of astaxanthin only did so in the UVB model. The combination of ascorbic acid and astaxanthin showed better antioxidative effect compared to each drug alone, suggesting a synergistic effect. View Full-Text
Keywords: antioxidant; ascorbic acid; astaxanthin; oxidative stress; diabetic retinopathy; retinal pigment epithelium; retinal disease antioxidant; ascorbic acid; astaxanthin; oxidative stress; diabetic retinopathy; retinal pigment epithelium; retinal disease
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MDPI and ACS Style

Oh, S.; Kim, Y.J.; Lee, E.K.; Park, S.W.; Yu, H.G. Antioxidative Effects of Ascorbic Acid and Astaxanthin on ARPE-19 Cells in an Oxidative Stress Model. Antioxidants 2020, 9, 833. https://doi.org/10.3390/antiox9090833

AMA Style

Oh S, Kim YJ, Lee EK, Park SW, Yu HG. Antioxidative Effects of Ascorbic Acid and Astaxanthin on ARPE-19 Cells in an Oxidative Stress Model. Antioxidants. 2020; 9(9):833. https://doi.org/10.3390/antiox9090833

Chicago/Turabian Style

Oh, Sanghyeon, Young J. Kim, Eun K. Lee, Sung W. Park, and Hyeong G. Yu 2020. "Antioxidative Effects of Ascorbic Acid and Astaxanthin on ARPE-19 Cells in an Oxidative Stress Model" Antioxidants 9, no. 9: 833. https://doi.org/10.3390/antiox9090833

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