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Article

Isolation, Characterization, and Antioxidant Activity Evaluation of a Fucoidan from an Enzymatic Digest of the Edible Seaweed, Hizikia fusiforme

1
Department of Marine Life Sciences, Jeju National University, Jeju, Jeju Self-Governing Province 63243, Korea
2
Marine Science Institute, Jeju National University, Jeju, Jeju Self-Governing Province 63333, Korea
3
Research Group of Process Engineering, Korea Food Research Institute, Wanju, Jeollabuk-do 55365, Korea
*
Authors to whom correspondence should be addressed.
Antioxidants 2020, 9(5), 363; https://doi.org/10.3390/antiox9050363
Received: 10 April 2020 / Revised: 24 April 2020 / Accepted: 26 April 2020 / Published: 27 April 2020
(This article belongs to the Special Issue Feature Papers in Antioxidants in 2020)
The previous study suggested that the sulfated polysaccharides from Hizikia fusiforme (HFPS) possess strong antioxidant activity. The purpose of this study is to isolate fucoidan from HFPS and to investigate its antioxidant activity. A fucoidan (HFPS-F4) with a molecular weight of 102.67 kDa was isolated from HFPS. HFPS-F4 contains 99.01% of fucoidan (71.79 ± 0.56% of carbohydrate and 27.22 ± 0.05% of sulfate content). The fucoidan increased the viability of H2O2-treated Vero cells by 5.41, 11.17, and 16.32% at the concentration of 12.5, 25, and 50 μg/mL, respectively. Further results demonstrated that this effect act diminishing apoptosis by scavenging intracellular reactive oxygen species (ROS) via increasing the expression of the endogenous antioxidant enzymes, which was induced by elevating total nuclear factor (erythroid-derived 2)-like 2 (Nrf2) levels. In addition, the in vivo test results displayed that the pretreatment of fucoidan improved the survival rates and decreased heart-beating rate, ROS, cell death, and lipid peroxidation in H2O2-stimulated zebrafish. Taken together, these results demonstrated that fucoidan isolated from HFPS has strong in vitro and in vivo antioxidant activities and it could be utilized in pharmaceutical, nutraceutical, and cosmeceutical industries. View Full-Text
Keywords: Hizikia fusiforme; fucoidan; oxidative stress; vero cells; zebrafish Hizikia fusiforme; fucoidan; oxidative stress; vero cells; zebrafish
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MDPI and ACS Style

Wang, L.; Jayawardena, T.U.; Yang, H.-W.; Lee, H.G.; Kang, M.-C.; Sanjeewa, K.K.A.; Oh, J.Y.; Jeon, Y.-J. Isolation, Characterization, and Antioxidant Activity Evaluation of a Fucoidan from an Enzymatic Digest of the Edible Seaweed, Hizikia fusiforme. Antioxidants 2020, 9, 363. https://doi.org/10.3390/antiox9050363

AMA Style

Wang L, Jayawardena TU, Yang H-W, Lee HG, Kang M-C, Sanjeewa KKA, Oh JY, Jeon Y-J. Isolation, Characterization, and Antioxidant Activity Evaluation of a Fucoidan from an Enzymatic Digest of the Edible Seaweed, Hizikia fusiforme. Antioxidants. 2020; 9(5):363. https://doi.org/10.3390/antiox9050363

Chicago/Turabian Style

Wang, Lei; Jayawardena, Thilina U.; Yang, Hye-Won; Lee, Hyo G.; Kang, Min-Cheol; Sanjeewa, K. K.A.; Oh, Jae Y.; Jeon, You-Jin. 2020. "Isolation, Characterization, and Antioxidant Activity Evaluation of a Fucoidan from an Enzymatic Digest of the Edible Seaweed, Hizikia fusiforme" Antioxidants 9, no. 5: 363. https://doi.org/10.3390/antiox9050363

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