Targeting PARK7 Improves Acetaminophen-Induced Acute Liver Injury by Orchestrating Mitochondrial Quality Control and Metabolic Reprogramming
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Cell Lines and Experimental Animals
2.3. Mice Infected with AAV Adeno-Associated Virus
2.4. Serum Analysis and Histological Examination
2.5. Frozen Section ROS and TUNNEL Experiments
2.6. Detection of the Cell Damage Index
2.7. Western Blotting and qPCR
2.8. Cell Transfection Experiment
2.9. Immunofluorescence
2.10. Electron Microscopy
2.11. Autophagy Flux Detection
2.12. Seahorse Experiment
2.13. Statistical Analysis
3. Results
3.1. Establishment of the APAP Acute Liver Injury Model
3.2. PARK7 Expression Increased in the APAP Acute Liver Injury Model
3.3. Silencing PARK7 Alleviated APAP-Induced Acute Liver Injury
3.4. Silencing PARK7 Promoted Mitochondrial Autophagy
3.5. The Protective Effect of PARK7 Silencing Was Not Due to Antioxidant Activity
3.6. The Protective Effect of Silencing PARK7 Was Related to Mitochondrial Synthesis and Metabolic Reprogramming
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cai, J.; Kong, D.; Long, Z.; Liu, J.; Liu, R.; Hai, C. Targeting PARK7 Improves Acetaminophen-Induced Acute Liver Injury by Orchestrating Mitochondrial Quality Control and Metabolic Reprogramming. Antioxidants 2022, 11, 2128. https://doi.org/10.3390/antiox11112128
Cai J, Kong D, Long Z, Liu J, Liu R, Hai C. Targeting PARK7 Improves Acetaminophen-Induced Acute Liver Injury by Orchestrating Mitochondrial Quality Control and Metabolic Reprogramming. Antioxidants. 2022; 11(11):2128. https://doi.org/10.3390/antiox11112128
Chicago/Turabian StyleCai, Jiao, Deqin Kong, Zi Long, Jiangzheng Liu, Rui Liu, and Chunxu Hai. 2022. "Targeting PARK7 Improves Acetaminophen-Induced Acute Liver Injury by Orchestrating Mitochondrial Quality Control and Metabolic Reprogramming" Antioxidants 11, no. 11: 2128. https://doi.org/10.3390/antiox11112128