Evaluation of Solid Supports for Slide- and Well-Based Recombinant Antibody Microarrays
AbstractAntibody microarrays have emerged as an important tool within proteomics, enabling multiplexed protein expression profiling in both health and disease. The design and performance of antibody microarrays and how they are processed are dependent on several factors, of which the interplay between the antibodies and the solid surfaces plays a central role. In this study, we have taken on the first comprehensive view and evaluated the overall impact of solid surfaces on the recombinant antibody microarray design. The results clearly demonstrated the importance of the surface-antibody interaction and showed the effect of the solid supports on the printing process, the array format of planar arrays (slide- and well-based), the assay performance (spot features, reproducibility, specificity and sensitivity) and assay processing (degree of automation). In the end, two high-end recombinant antibody microarray technology platforms were designed, based on slide-based (black polymer) and well-based (clear polymer) arrays, paving the way for future large-scale protein expression profiling efforts. View Full-Text
- Supplementary File 1:
PDF-Document (PDF, 1433 KB)
Share & Cite This Article
Gerdtsson, A.S.; Dexlin-Mellby, L.; Delfani, P.; Berglund, E.; Borrebaeck, C.A.K.; Wingren, C. Evaluation of Solid Supports for Slide- and Well-Based Recombinant Antibody Microarrays. Microarrays 2016, 5, 16.
Gerdtsson AS, Dexlin-Mellby L, Delfani P, Berglund E, Borrebaeck CAK, Wingren C. Evaluation of Solid Supports for Slide- and Well-Based Recombinant Antibody Microarrays. Microarrays. 2016; 5(2):16.Chicago/Turabian Style
Gerdtsson, Anna S.; Dexlin-Mellby, Linda; Delfani, Payam; Berglund, Erica; Borrebaeck, Carl A.K.; Wingren, Christer. 2016. "Evaluation of Solid Supports for Slide- and Well-Based Recombinant Antibody Microarrays." Microarrays 5, no. 2: 16.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.