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Article

In Vitro Assessment of Kurdish Rice Genotypes in Response to PEG-Induced Drought Stress

1
Department of Biotechnology and Crop Science, College of Agricultural Engineering Sciences, University of Sulaimani, Kurdistan Region of Iraq, Sulaymaniyah 46001, Iraq
2
Department of Plant Biology, Faculty of AgriSciences, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic
3
Department of Horticulture, College of Agricultural Engineering Sciences, University of Sulaimani, Kurdistan Region of Iraq, Sulaymaniyah 46001, Iraq
4
Department of Phytochemistry Centre of Region Haná for Biotechnological and Agricultural Research Faculty of Science, Palacky University in Olomouc Slechtitelu 27, 78371 Olomouc, Czech Republic
*
Author to whom correspondence should be addressed.
Appl. Sci. 2020, 10(13), 4471; https://doi.org/10.3390/app10134471
Received: 30 May 2020 / Revised: 23 June 2020 / Accepted: 23 June 2020 / Published: 28 June 2020
(This article belongs to the Special Issue Applied Agri-Technologies)
Rice (Oryza sativa L.) is productively affected by different environmental factors, including biotic and abiotic stress. The objectives of this research were to evaluate the genetic distinction among Kurdish rice genotypes using the simple sequence repeats (SSRs) molecular markers and to perform in vitro tests to characterize the drought tolerance of six local rice genotypes. The polymorphic information content (PIC) varied from 0.38 to 0.84 with an average of 0.56. The genetic distance ranged from 0.33 to 0.88. Drought stress had a significant impact (p ≤ 0.05) on callus growth parameters. Enzymatic antioxidant systems were predicted and exhibited a significant variation. The findings revealed that proline levels increase in proportion to polyethylene glycol (PEG) concentrations. Kalar and Gwll Swr genotypes showed the worst performances in phenotypic and biochemical traits, while Choman and Shawre exhibited the best phenotypic and biochemical performances. A positive and substantial relationship between callus fresh weight (CFW) and callus dry weight (CDW) was found under stressful and optimized conditions. Callus induction (CI) was positively and significantly associated with the catalase activity (CAT) in all stressed treatments. Based on the results for callus growth and the biochemical parameters under stress conditions, a remarkable genotype distinction, based on the tolerance reaction, was noted as follows: PEG resistant > susceptible, Choman > Shawre > White Bazyan > Red Bazyan > Gwll Swr > Kalar. The CI and CAT characteristics were considered as reliable predictors of drought tolerance in rice genotypes. View Full-Text
Keywords: rice; SSR markers; drought stress; callus growth; proline; antioxidant enzymes; inorganic elements rice; SSR markers; drought stress; callus growth; proline; antioxidant enzymes; inorganic elements
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MDPI and ACS Style

Rahim, D.; Kalousek, P.; Tahir, N.; Vyhnánek, T.; Tarkowski, P.; Trojan, V.; Abdulkhaleq, D.; Ameen, A.H.; Havel, L. In Vitro Assessment of Kurdish Rice Genotypes in Response to PEG-Induced Drought Stress. Appl. Sci. 2020, 10, 4471. https://doi.org/10.3390/app10134471

AMA Style

Rahim D, Kalousek P, Tahir N, Vyhnánek T, Tarkowski P, Trojan V, Abdulkhaleq D, Ameen AH, Havel L. In Vitro Assessment of Kurdish Rice Genotypes in Response to PEG-Induced Drought Stress. Applied Sciences. 2020; 10(13):4471. https://doi.org/10.3390/app10134471

Chicago/Turabian Style

Rahim, Didar; Kalousek, Petr; Tahir, Nawroz; Vyhnánek, Tomáš; Tarkowski, Petr; Trojan, Vaclav; Abdulkhaleq, Dana; Ameen, Ahmad H.; Havel, Ladislav. 2020. "In Vitro Assessment of Kurdish Rice Genotypes in Response to PEG-Induced Drought Stress" Appl. Sci. 10, no. 13: 4471. https://doi.org/10.3390/app10134471

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