Impact of Microbial Protease Enzyme and Dietary Crude Protein Levels on Growth and Nutrients Digestibility in Broilers over 15–28 Days
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethical Approval
2.2. Experimental Procedures
2.3. Total Tract Digestibility Assay
2.4. Statistical Analysis
3. Results
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Acknowledgments
Conflicts of Interest
References
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Ingredients (%) | Diet 17% CP | Diet 19% CP | Diet 21% CP |
---|---|---|---|
Corn | 62.45 | 59.62 | 57.18 |
Soybean meal (44% CP) | 9.27 | 11.08 | 14.51 |
Canola meal | 7.00 | 7.00 | 8.00 |
Sunflower meal | 5.71 | 6.00 | 5.00 |
Gluten meal (60% CP) | 4.50 | 2.50 | 3.00 |
Poultry by-products | 2.00 | 3.00 | 3.00 |
Gluten meal (40% CP) | 1.21 | 2.60 | 2.81 |
Vegetable oil | 1.00 | 2.19 | 2.04 |
Bone meal | 1.00 | 1.00 | 1.00 |
Dicalcium Phosphate | 1.00 | 1.00 | 0.65 |
Limestone | 1.00 | 0.86 | 1.02 |
Salt | 0.45 | 0.45 | 0.45 |
VitaVit-minerals premix 1 | 0.12 | 0.12 | 0.12 |
L-Lys HCl | 0.55 | 0.42 | 0.23 |
DL-Met | 0.28 | 0.20 | 0.14 |
Valine | 0.99 | 0.71 | 0.46 |
Threonine | 0.72 | 0.66 | 0.34 |
Isoleucine | 0.43 | 0.36 | 0.00 |
Arginine | 0.32 | 0.22 | 0.00 |
Chemical composition | |||
ME, kcal/kg | 2950 | 2930 | 2920 |
Crude protein, % | 17.00 | 19.00 | 21.00 |
Ca, % | 1.00 | 1.00 | 1.00 |
Available P, % | 0.45 | 0.45 | 0.45 |
Met + Cys, % | 0.90 | 0.90 | 0.90 |
Methionine, % | 0.59 | 0.56 | 0.53 |
Lysine, % | 1.15 | 1.15 | 1.15 |
Valine, % | 0.96 | 0.68 | 0.99 |
Threonine, % | 0.83 | 0.72 | 0.89 |
Isoleucine, % | 0.93 | 0.85 | 0.88 |
Arginine, % | 1.19 | 1.09 | 1.21 |
Item | Body Weight Gain (g) | Feed Intake (g) | FCR (g/g) |
---|---|---|---|
CP 1 | |||
CP-17 | 938.0 b | 1895.0 | 2.02 a |
CP-19 | 1098.4 a | 1857.1 | 1.69 b |
CP-21 | 1096.0 a | 1873.2 | 1.71 b |
Pooled SEM | 76.33 | 102.45 | 0.11 |
Enzyme 2 | |||
Without enzyme | 1021.0 b | 1867.2 | 1.84 a |
CP + enzyme | 1067.0 a | 1883.1 | 1.77 b |
Pooled SEM | 45.66 | 124.00 | 0.12 |
CP × Enzyme | |||
CP-170 | 899.0 d | 1875.0 ab | 2.08 a |
CP-17E | 977.3 c | 1915.4 a | 1.96 b |
CP-190 | 1082.2 b | 1858.0 ab | 1.71 c |
CP-19E | 1115.0 a | 1856.4 b | 1.66 d |
CP-210 | 1082.4 b | 1869.0 ab | 1.72 c |
CP-21E | 1109.00 ab | 1877.4 ab | 1.69 cd |
Pooled SEM | 78.33 | 106.55 | 0.11 |
p-Value | |||
CP | <0.001 | 0.1799 | <0.001 |
Enzyme | 0.0001 | 0.3301 | 0.0001 |
CP × Enzyme | 0.0456 | 0.0383 | 0.0238 |
Item | Total Tract N Retention (%) | GE (Kcal/kg) | Abdominal Fat (%) |
---|---|---|---|
CP 1 | |||
CP-17 | 66.00 b | 2889.0 b | 0.87 a |
CP-19 | 68.00 a | 2928.0 a | 0.59 b |
CP-21 | 68.25 a | 2930.0 a | 0.55 c |
Pooled SEM | 1.74 | 103.55 | 0.01 |
Enzyme 2 | |||
Without enzyme | 66.08 b | 2904.0 | 0.72 a |
CP + enzyme | 68.29 a | 2927.1 | 0.62 b |
Pooled SEM | 2.42 | 203.45 | 0.01 |
CP × Enzyme | |||
CP-170 | 62.71 d | 2876.0 b | 0.96 a |
CP-17E | 64.00 c | 2902.0 ab | 0.77 b |
CP-190 | 67.00 b | 2919.0 ab | 0.63 c |
CP-19E | 68.19 ab | 2936.5 a | 0.55 d |
CP-210 | 68.18 ab | 2917.0 ab | 0.57 d |
CP-21E | 68.36 a | 2943.2 a | 0.53 d |
Pooled SEM | 3.33 | 107.00 | 0.01 |
p-value | |||
CP | <0.001 | <0.001 | 0.0348 |
Enzyme | 0.0007 | <0.001 | 0.0919 |
CP × Enzyme | 0.0339 | 0.0009 | 0.0052 |
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Jabbar, A.; Tahir, M.; Alhidary, I.A.; Abdelrahman, M.A.; Albadani, H.; Khan, R.U.; Selvaggi, M.; Laudadio, V.; Tufarelli, V. Impact of Microbial Protease Enzyme and Dietary Crude Protein Levels on Growth and Nutrients Digestibility in Broilers over 15–28 Days. Animals 2021, 11, 2499. https://doi.org/10.3390/ani11092499
Jabbar A, Tahir M, Alhidary IA, Abdelrahman MA, Albadani H, Khan RU, Selvaggi M, Laudadio V, Tufarelli V. Impact of Microbial Protease Enzyme and Dietary Crude Protein Levels on Growth and Nutrients Digestibility in Broilers over 15–28 Days. Animals. 2021; 11(9):2499. https://doi.org/10.3390/ani11092499
Chicago/Turabian StyleJabbar, Abdul, Muhammad Tahir, Ibrahim A. Alhidary, Mutassim A. Abdelrahman, Hani Albadani, Rifat Ullah Khan, Maria Selvaggi, Vito Laudadio, and Vincenzo Tufarelli. 2021. "Impact of Microbial Protease Enzyme and Dietary Crude Protein Levels on Growth and Nutrients Digestibility in Broilers over 15–28 Days" Animals 11, no. 9: 2499. https://doi.org/10.3390/ani11092499
APA StyleJabbar, A., Tahir, M., Alhidary, I. A., Abdelrahman, M. A., Albadani, H., Khan, R. U., Selvaggi, M., Laudadio, V., & Tufarelli, V. (2021). Impact of Microbial Protease Enzyme and Dietary Crude Protein Levels on Growth and Nutrients Digestibility in Broilers over 15–28 Days. Animals, 11(9), 2499. https://doi.org/10.3390/ani11092499